FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003246692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181101
Sequences flagged as poor quality0
Sequence length300
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT51742.856969315464851No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT46432.563762762215559No Hit
CAGGAAACAGCTATGACCAGGCAGTAACTTGGGCATTTCTTCTGATTCTC40162.217547114593514No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT34361.8972838360914628No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA32261.7813264421510648No Hit
CAGGAAACAGCTATGACCGGGACCAGTCCAATAACCCCTGAGGGGTATAT31541.7415696213714997No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGGCTGTTT31091.7167216083842716No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT30421.679725677936621No Hit
CAGGAAACAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT29681.6388645010242904No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGCACTCCTTC27551.5212505728847439No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC26831.481493752105179No Hit
CAGGAAACAGCTATGACCTGGAAACCCATGCCATGCACTCAACACTACAA23511.2981706340660737No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT23141.2777400456099084No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA22261.2291483757682178No Hit
CAGGAAACAGCTATGACCAGCAACATACCGTTTGTAAACAACTGGCCGTC19841.0955212837035688No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTCCCCAACCATAG19811.09386474950442No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG19161.0579731751895352No Hit
CAGGAAACAGCTATGACCATCGCACCACTGCACTCCAGCGCTCCATACAC18951.0463774357954954No Hit
CAGGAAACAGCTATGACCAGCGTCTCATTCCATAAAGGCCACCATGAAAC18481.0204250666755015No Hit
CAGGAAACAGCTATGACCTCCTGCCCCACCCTGATACAGGTCACTGTGGA18431.0176641763435872No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCCATGCTCACCT18291.0099336834142274No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC18251.007724971148696No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT16870.931524397987863No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGTGCACCTCCTTC16740.9243460831248862No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACCCTGTCTCTCTAGC16060.8867979746108525No Hit
CAGGAAACAGCTATGACCCCTGGACGCAATCACCCAGGCTGCAGTGCGGT15260.8426237293002247No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA15190.8387584828355449No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG14600.8061799769189568No Hit
CAGGAAACAGCTATGACCTTGGACAGCACCTTGAATGCTATCAGGGAAGC14380.7940320594585342No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG13280.733292472156421No Hit
CAGGAAACAGCTATGACCCTGGAATTTCAACAGAATGGCATTTATATGAT13210.7294272256917411No Hit
CAGGAAACAGCTATGACCCAGGGCTCCAAGAATGTTCCACACATCTTTCT13040.7200401985632326No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG12520.6913269391113247No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCCGGAGAGCCTGT12230.675313775186222No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTTGCAGTGAGCCG11730.6477048718670797No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAACTGGCCAGCCTG11520.6361091324730399No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG11090.6123654756185775No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGGTCCTCCTC10850.5991132020253891No Hit
CAGGAAACAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC10230.5648781619096526No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA9790.5405823269888074No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT9790.5405823269888074No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCTGTCCAGCCCC9720.5367170805241274No Hit
CAGGAAACAGCTATGACCGGCAGACAGCCTCTCCTCCTTTCATCACAAAA9550.527330053395619No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTCCCTTTGGGCT9530.5262256972628533No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCAGGAAACAGCT9110.5030342184747737No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACACACGGCTCCTCA8990.49640808167817957No Hit
CAGGAAACAGCTATGACCGCTAATGCTTACTCTTTGCGTGAGTACTGGTT8900.49143847908073396No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTGCAGTGAACTGA8810.48646887648328835No Hit
CAGGAAACAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG8750.48315580808499126No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCCTGAGAGCCTGT8660.47818620548754565No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATGAGTTAGCCAGC8560.4726644248237172No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTGGCATGAAGGA8320.4594121512305288No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA7940.43842938470798065No Hit
CAGGAAACAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG7650.42241622078287805No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAACTGGCCGTCGTT7550.4168944401190496No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCAAGCCTGACTG7430.41026830332245545No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTATTGCAGCCTGT6870.379346331605016No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGGCGAGAGCACCC6850.3782419754722503No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGCGGAAGAGGAGCC6750.37272019480842183No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGATTCTCAGATTCC6740.372168016742039No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGACACACCACT6660.3677505922109762No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTCCCTGTGCCCAT6600.36443752381267913No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGACACTGCAGCCTGGG6580.3633331676799134No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTTCCCTTTGGGC6430.3550504966841707No Hit
CAGGAAACAGCTATGACCTCCAGCCAGCCAGTCCTTGTGTTGATGGCCTC6400.3533939624850222No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA6370.3517374282858736No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT6270.34621564762204515No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC6250.34511129148927944No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCATAGGGGCATCC5960.3290981275641769No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTAGGAGGGCTGCG5960.3290981275641769No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGATACTACTA5860.3235763469003484No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCATGGAGGCCCTC5840.3224719907675827No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGGGCGCAGCCACC5700.31474149783822286No Hit
CAGGAAACAGCTATGACCCACCGCTGTCCCTTCCCAAGGGGCTGAAGGTG5620.3103240733071601No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTGACCCTGGCAG5400.29817615584673746No Hit
CAGGAAACAGCTATGACCCCTGGACGCAATCACCCAGGCTGGAGTGCAGT5400.29817615584673746No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGGCTGCTT5400.29817615584673746No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGCTTCCCATCTCA5390.29762397778035465No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT5380.29707179971397174No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT5240.2893413067846119No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATCCAAACCAG5200.2871325945190805No Hit
CAGGAAACAGCTATGACCACCTTTACCCAAATAGCTACCACCAGTGCCTT5160.2849238822535491No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGACGTGTCCGCT4800.26504547186376665No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTGCTTGAGCCCAG4700.2595236911999382No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGGCTCTTTT4640.2562106228016411No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGATGCTGCCTCCT4570.25234537633696114No Hit
CAGGAAACAGCTATGACCTGCTGCTTTTCCTGCCAGAAGACAGAAGCCCA4480.24737577373951553No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCGGAGAGCCTGTG4450.24571923954036698No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTTGGAGTCCCAC4430.24461488340760126No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCTGTCACACACCC4390.2424061711420699No Hit
CAGGAAACAGCTATGACCATCGCACCACTGCACTCCAGCACTGGCCGTCG4290.23688439047824142No Hit
CAGGAAACAGCTATGACCTGCTTTGGGGCCATGGAGTTGGGGGAATGGGA4140.22860171948249874No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCCAGAGAGCACCCG4120.227497363349733No Hit
CAGGAAACAGCTATGACCCCTGGACGCAATCACCCAGGCCAGCTAAGTGA4070.2247364730178188No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTACTGGCATGAA4000.22087122655313884No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA3920.2164538020220761No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCAAGCCTGACAG3920.2164538020220761No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACTCTTGGCCTGCT3840.2120363774910133No Hit
CAGGAAACAGCTATGACCTCCAGTAAAACTGCCTCTGTTTCAACTCCCTT3800.2098276652254819No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGCACCCCCCCATC3800.2098276652254819No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAACAGCCTGGCCAA3780.2087233090927162No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGACTGGCCGTCGTT3550.19602321356591074No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTTGGCATGAAGG3500.1932623232339965No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATTCTCCATCTCCA3480.19215796710123081No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGACCAGTGAGCGTA3440.1899492548356994No Hit
CAGGAAACAGCTATGACCGGGCAGGCCGAGGACATTAGAAATTTGGGACG3250.1794578715744253No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAATTTCATTCT3210.17724915930889393No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG3200.1766969812425111No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGAACAAAAAGCCTCAC3100.1711752005786826No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTGCTCATAGGCAG3050.16841431024676837No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAATGAAACTTGGTT3000.16565341991485416No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGATTCTTTT2980.16454906378208844No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTTGTAATCCCATC2960.16344470764932276No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGCTCAAGG2930.1617881734501742No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGGTGATGG2890.15957946118464283No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATTGCCTCATGTCC2740.15129679018890013No Hit
CAGGAAACAGCTATGACCTTCCTGATGGGATTCAGCCATCAACAGTAGGA2680.14798372179060304No Hit
CAGGAAACAGCTATGACCTGCTTTGGGGCCATGGAGTTGAGCTGTTGAGA2670.14743154372422018No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTAGGAGGGCTGTG2670.14743154372422018No Hit
CAGGAAACAGCTATGACCGGGACCAGTCCAATAACCCCTGACAGGAAACA2650.1463271875914545No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGACAAGTGCATGG2650.1463271875914545No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTTATATGCCTCCT2630.1452228314586888No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTACAGTGAGCCGA2550.14080540692762603No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTAGTGGCATGAA2520.13914887272847748No Hit
CAGGAAACAGCTATGACCGCAGCCACCCTGAACACACTTGGCTATCACCC2520.13914887272847748No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAATTCCATTCT2480.1369401604629461No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCGTGCTAGGCCA2470.13638798239656325No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACTAGAAGTAATTA2470.13638798239656325No Hit
CAGGAAACAGCTATGACCGTCCTCAGATAGCCAGGCTCTTGGTGTCAAGT2460.13583580433018042No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATCTAAACCAG2400.13252273593188332No Hit
CAGGAAACAGCTATGACCACCCAGTTAGAGCCATGGTTCCTGTAGGCTAG2280.12589659913528914No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTCCCCACTGGCAG2250.1242400649361406No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAACCAGCCTGGCCA2180.12037481847146068No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG2180.12037481847146068No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGAGCCCAGGAAGTTGA2020.11153996940933511No Hit
CAGGAAACAGCTATGACCATCGCACCACTGCACTCCAGCAGGAAACAGCT1940.10712254487827236No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCCGGCAAACTTGG1910.1054660106791238No Hit
CAGGAAACAGCTATGACCGCAGCCACCCTGAACACACTCCACCAGGAAGA1880.10380947647997527No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTTGCGGTGAGCCG1870.10325729841359241No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGTCAGGTCCAAGT1830.10104858614806103No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)