FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128314
Sequences flagged as poor quality0
Sequence length300
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTAGCACGAAAAGTAGGTGGCCTTACTGGTAGCA35152.7393737238337206No Hit
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA29052.263977430366133No Hit
TGTAAAACGACGGCCAGTTCCCAAAGGCTGGTGGAGGAGATGCTGAAGAT26072.0317346509344265No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGTGGGAGCCCC26042.029396636376389No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTGGTGGTCC25251.9678289196814067No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT24501.9093785557304737No Hit
TGTAAAACGACGGCCAGTCAGCCAGTAGCAACAGCAGCTACTGATGTAAG22611.7620836385741228No Hit
TGTAAAACGACGGCCAGTGGGGAGTGAGAGGCCATAGCTGTCTGGCATGG22311.7387034929937497No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG21321.6615490125785184No Hit
TGTAAAACGACGGCCAGTCCTGGAACTGCATCATCAGGATCTTCACGATT20691.6124507068597347No Hit
TGTAAAACGACGGCCAGTAGTCAGCCACGTTGGAACCAGTACTCACGCAA20251.5781598266751875No Hit
TGTAAAACGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGTTGCTGAT19511.520488800910267No Hit
TGTAAAACGACGGCCAGTCAAGCAGACAGAGAGCTGAAGGAGTGCCAAGG19471.5173714481662173No Hit
TGTAAAACGACGGCCAGTCCCAGCACAACTTCCCCCATGGCGTGGTGTCC18491.4409963059369983No Hit
TGTAAAACGACGGCCAGTTGGAACCATTTTTGATAACTTTCTGATCACAG18311.4269682185887744No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCATGACAAGGCT15711.2243402902255405No Hit
TGTAAAACGACGGCCAGTTGTAGCCCCCAGGAAGAGGGCGCGGAGGCAGC15281.1908287482270055No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGCCTGGGTGGGA15051.1729039699487196No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC13251.0326230964664806No Hit
TGTAAAACGACGGCCAGTGGCTCCCCAGCTGTCTCTCTTGTAAAACGACG13181.0271677291643937No Hit
TGTAAAACGACGGCCAGTTGAGAAGCTGAGGCTTAAAGAGGGATCCCTTG13091.0201536854902815No Hit
TGTAAAACGACGGCCAGTACTAAACTGTAGTGAACAGAGACATGCCCAGG13011.0139189800021822No Hit
TGTAAAACGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT11340.883769502938105No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCCAAGAGGTGGGG11050.8611686955437443No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCAGCAGGATGGAG9820.7653100986642143No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG9580.7466059821999158No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTCAGTTCACT9390.7317985566656795No Hit
TGTAAAACGACGGCCAGTGCCAATCCTGAGGCCATCAACACAAGGACTGG9280.7232258366195427No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG9170.7146531165734059No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCAGCCTGGGTGAT8850.6897142946210079No Hit
TGTAAAACGACGGCCAGTGTTCTACCGTCAGTCCTGGCATTATTTCTCTC8740.681141574574871No Hit
TGTAAAACGACGGCCAGTTGCCTTGAAGATTTTCTGTAGGATAATTTTGG8500.6624374581105725No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA8370.6523060616924108No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT8220.6406159889022242No Hit
TGTAAAACGACGGCCAGTCAGTTCCCAGGGACTCTGCTCTATGAGAGAGA7340.5720342285331297No Hit
TGTAAAACGACGGCCAGTTCATAGCTGTGAAGAGGGATACCAAGGAAAGT7290.5681375376030675No Hit
TGTAAAACGACGGCCAGTAAGGGTGGGTCATGGGGTGACACCGTAACTGT7230.5634615084869928No Hit
TGTAAAACGACGGCCAGTCCCTGTCCTGAGCACCGTTGCTAACCCCCAGT7060.5502127593247813No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGACCAACAATGGCCTG6980.5439780538366819No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGGTTGGAGGTG6870.535405333790545No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC6830.5322879810464953No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGACGGCACTGGTG6650.5182598936982714No Hit
TGTAAAACGACGGCCAGTCCTGCCGCCTGGTCTCACCTCGCCATGGTTCG6440.5018937917920102No Hit
TGTAAAACGACGGCCAGTCAAGCAGACAGAGAGCTGAAGGAGGAGGACCC6370.49643842448992315No Hit
TGTAAAACGACGGCCAGTTACAGGCCCCACACTGCTGACTCTGCTCCCCT6350.49487974811789825No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT6210.4839690135137241No Hit
TGTAAAACGACGGCCAGTAAGGGTGGGTCATGGGGTGAGGGTGGGTCGCG6160.4800723225836619No Hit
TGTAAAACGACGGCCAGTTTGATGCAAGAAAAAGAAACTGGACTTAATGT6150.4792929843976495No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCACTTCCTGC5930.46214754430537586No Hit
TGTAAAACGACGGCCAGTTCCATTACTCTACACAAGCAGGGTGGGGTGCT5900.4598095297473385No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC5820.45357482425923906No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC5630.43876739872500276No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGCGCAGCCCTCCTAC5550.4325326932369032No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC5520.43019467867886596No Hit
TGTAAAACGACGGCCAGTTTGCTGCTGGGCTTCCTCTGCACTTGAAAGGA5480.4270773259348161No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG5430.423180635004754No Hit
TGTAAAACGACGGCCAGTGGAGTGGCTGCCAGCAAGAGACAGTGTTCAGA5270.410711224028555No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG5040.3927864457502689No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA4830.3764203438440077No Hit
TGTAAAACGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT4820.37564100565799524No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCATAGCTGTTTC4740.36940630016989573No Hit
TGTAAAACGACGGCCAGTCCTGCCGCCTGGTCTCACCTCGCTATGGTTCG4730.3686269619838833No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTCGGCTCACT4680.3647302710538211No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGCGTGGGGCACTC4570.35615755100768426No Hit
TGTAAAACGACGGCCAGTAGGTGAAGAAGAAAAGACAAAGACCTACAGTG4540.353819536449647No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCACATGCCTCCC4480.3491435073335723No Hit
TGTAAAACGACGGCCAGTGCCCTGAACCGGTGCCAAATGGAGTGAAGAAA4450.346805492775535No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGACCAGACG4280.33355674361332355No Hit
TGTAAAACGACGGCCAGTACATGCGTGGCCCTGTCTACTGGGCTCCAGTA4260.3319980672412987No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAAGCCCCAGACCC4230.3296600526832614No Hit
TGTAAAACGACGGCCAGTTTGCTGCTGGGCTTCCTCTGCCCTGAGGGCTT4010.31251461259098773No Hit
TGTAAAACGACGGCCAGTCCACCTGCTTCGCACCATCTCCATCTCCATTT3970.309397259846938No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCAAGAGGTGGGGT3960.30861792166092555No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCTCCTTCCCAT3940.30705924528890066No Hit
TGTAAAACGACGGCCAGTTGGAGTGCCTGGGTTAGAACCCTGCTGGTACC3860.30082453980080115No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGTCAT3850.3000452016147887No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC3800.29614851068472653No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCTGCAGACAGGAT3740.2914724815686519No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGACATTCCTGCAA3630.2828997615225151No Hit
TGTAAAACGACGGCCAGTTCCCACCACCATACCCCAACTGTAAAACGACG3530.27510637966239065No Hit
TGTAAAACGACGGCCAGTTCTTTGGCTCTACCTAAAAGTTGGTCATAGCT3490.2719890269183409No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGAAGCCCAGTCAGG3410.26575432143024147No Hit
TGTAAAACGACGGCCAGTACCGGGCCAGGTCAGTTACTCCCGACGCCAGG3270.2548435868260673No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGTGTAAAACGACG3130.24393285222189318No Hit
TGTAAAACGACGGCCAGTTCTTTGGCTCTACCTAAAAGTTGGTCAGCAAA3100.24159483766385587No Hit
TGTAAAACGACGGCCAGTGGTCCCACTCTGGCTTTCCCTGAGCGAGATAA3090.24081549947784342No Hit
TGTAAAACGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCAAGGAATTCAG3010.2345807939897439No Hit
TGTAAAACGACGGCCAGTACCGGGCCAGGTCAGTTACTGCCAGTACCGGG2770.21587667752544537No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG2760.21509733933943298No Hit
TGTAAAACGACGGCCAGTCCCTGTCCTGAGCACCGTTGGCTCCAGGATCG2710.21120064840937075No Hit
TGTAAAACGACGGCCAGTTCTTCACCTCCCAAAATGCTGGGATTACAGGC2690.2096419720373459No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGTGACCCTTCCAGGA2690.2096419720373459No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA2680.20886263385133347No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG2670.20808329566532102No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTCCCTTCTCTTTG2620.20418660473525882No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGCTGTGGAGATGG2620.20418660473525882No Hit
TGTAAAACGACGGCCAGTGTCCCAGGTGTATGGAGCGCAGTGGTGCGATG2610.20340726654924637No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCGCCCCATCTGA2570.20028991380519665No Hit
TGTAAAACGACGGCCAGTACATGCGTGGCCCTGTCTACTCATCCCCCTCT2460.19171719375905982No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTCAGCTCACT2440.19015851738703493No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGACAGCCTGGGTGG2400.18704116464298517No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAGAGCAAAGTCC2390.18626182645697273No Hit
TGTAAAACGACGGCCAGTTCCATCCACTGCTGAGAAATCTCCAGGTCATA2390.18626182645697273No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAGTAGGAGAG2380.1854824882709603No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCCAGGATGGAGGG2360.18392381189893542No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCACTAGGAGAG2360.18392381189893542No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCTGGTGCCTCCG2350.18314447371292297No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCACTCCAGCCTGG2260.17613043003881104No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGACGTGCTGGTGAA2260.17613043003881104No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCAGTGGTGCGATGGTCATAGCTG2260.17613043003881104No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTAGGGTTGGGTCA2230.17379241548077373No Hit
TGTAAAACGACGGCCAGTGGGAGGCCATGTTGAACTTATTCAGAATGTAC2210.17223373910874887No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGCTAACTCATAGG2190.17067506273672398No Hit
TGTAAAACGACGGCCAGTCCCAGCACAACTTCCCCCATGTAAAACGACGG2110.16444035724862446No Hit
TGTAAAACGACGGCCAGTGGTGTTACCGTGGTCACCCGCAGGGACACGGA2060.16054366631856226No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACACTGCCGAGTG2060.16054366631856226No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG2050.15976432813254984No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCTCCCTGCCCCGC2030.15820565176052495No Hit
TGTAAAACGACGGCCAGTCCCCCAGAAAATTGTGCCAAAGAGTTTAGAAA2000.15586763720248767No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC1970.15352962264445033No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCCCTCCAGCCTGG1970.15352962264445033No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTGGGGTTAGGCAT1970.15352962264445033No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGGACTGTGAGGCCCT1950.15197094627242544No Hit
TGTAAAACGACGGCCAGTTTGCTGCTGGGCTTCCTCTGGGCTGCTCTGTC1920.14963293171438816No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCTGGAAGCCCGCC1900.14807425534236326No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCATGGCCTGGTGGT1900.14807425534236326No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGCGCTCCCCACCTCC1880.1465155789703384No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTGGGTGGGGTTAG1870.14573624078432595No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCATCAGGTTCCC1860.1449569025983135No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGTGTCGTGGGGCA1840.14339822622628864No Hit
TGTAAAACGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCATAAACGCAAG1800.1402808734822389No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAGGAGAGTGA1800.1402808734822389No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCATCATGTTCC1780.138722197110214No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT1720.13404616799413938No Hit
TGTAAAACGACGGCCAGTGGCTCTATATCAGGGCCCGCCGGTCATAGCTG1650.1285908006920523No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCCCCCCAGGATGG1580.12313543338996523No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGCCTAGCACGACG1560.12157675701794037No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCAGGGCTTCAC1560.12157675701794037No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTTCCTATCG1540.12001808064591547No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAGAGCAAAGTC1530.11923874245990304No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGGACACCAGTGA1520.11845940427389061No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCAGGATGGAGGGG1510.11768006608787818No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGCACGGCACTGGT1500.11690072790186573No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGATATCTCCTGCAC1460.11378337515781597No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGAGCAAAGT1440.11222469878579111No Hit
TGTAAAACGACGGCCAGTTCCATTACTCTACACAAGCAGGGTACACTTAG1420.11066602241376625No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGAGGCTCCTTCC1390.10832800785572892No Hit
TGTAAAACGACGGCCAGTACCGGGCCAGGTCAGTTACTGTACCGGGGCCA1380.10754866966971649No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGGGACGTGGGGCA1360.1059899932976916No Hit
TGTAAAACGACGGCCAGTCCACCTGCTTCGCACCATCTCGGCCCAGCCCA1360.1059899932976916No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG1350.10521065511167917No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGACAGCAGCTCTGG1330.10365197873965427No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGTGAGGAGTTACTGT1330.10365197873965427No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAAGGAGAGTG1300.10131396418161698No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)