FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113224
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT22752.0092913163286936No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC17961.586236133681905No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG17881.5791704938882216No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT17801.5721048540945384No Hit
CAGGAAACAGCTATGACCTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT17701.5632728043524342No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC16361.4449233378082385No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC16041.4166607786335053No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC15051.3292234861866743No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA14951.32039143644457No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA14721.3000777220377304No Hit
CAGGAAACAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT14691.2974281071150993No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA14121.2470854235851057No Hit
CAGGAAACAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAATT13791.217939659436162No Hit
CAGGAAACAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAACT13031.1508160813961703No Hit
CAGGAAACAGCTATGACCCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT12491.103123012788808No Hit
CAGGAAACAGCTATGACCGCCCAGCAATGGAGAGGCTACCAGCTGATCCA11361.0033208507030311No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACCAGGGCAGCGGT11260.9944888009609271No Hit
CAGGAAACAGCTATGACCCCGTAGCCACGCCCCTATTGTCTGACCACGCT10440.9220659930756729No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC10350.9141171483077793No Hit
CAGGAAACAGCTATGACCTGGGCCTGGACACTTTGACGGCGGGGCACGTC9790.864657669751996No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT9240.8160813961704233No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG9110.8045997315056879No Hit
CAGGAAACAGCTATGACCTCCCACCACTGACTCCAGGGGAAGGAGGAGGA8980.7931180668409525No Hit
CAGGAAACAGCTATGACCGTCCTAGCACCGCAGCGAATGGGACCCGCAAG8200.72422807885254No Hit
CAGGAAACAGCTATGACCGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC8200.72422807885254No Hit
CAGGAAACAGCTATGACCTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT8070.7127464141878047No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT7980.7047975694199109No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC7970.7039143644457005No Hit
CAGGAAACAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG7840.6924326997809651No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGCACTCCTTC7310.6456228361478131No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGGCTGTTT7240.6394404013283402No Hit
CAGGAAACAGCTATGACCGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA7160.632374761534657No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCTGCCCAACACCC6700.5917473327209779No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACCCTTTGTCCA6580.5811488730304528No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC6550.5784992581078217No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT6550.5784992581078217No Hit
CAGGAAACAGCTATGACCAGGGCAAGTGTTCGCGTTGTAGGCGGCGGGAC6550.5784992581078217No Hit
CAGGAAACAGCTATGACCTGGGGATGAGGACAGCAGGGCAGTCACCATCT6540.5776160531336112No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCCCCTCTTTTAGG6460.570550413339928No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCCCCTCTTCTAGG6280.5546527238041405No Hit
CAGGAAACAGCTATGACCGCAGTGACAGGGCAAGGAGGGTGGATGGATCC6080.5369886243199322No Hit
CAGGAAACAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT6040.5334558044230905No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACGGGGATGGCAGTG5960.5263901646294071No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT5910.5219741397583552No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACCCTGTCTCTCTAGC5790.5113756800678301No Hit
CAGGAAACAGCTATGACCGCTCCTGTGCTGTCAAGGGACTCCTGTAGTGG5340.47163145622836145No Hit
CAGGAAACAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC5310.4689818413057302No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCTGAGGCAGGAGA5240.46279940648625734No Hit
CAGGAAACAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC5220.46103299653783647No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT5220.46103299653783647No Hit
CAGGAAACAGCTATGACCCACGACACCCCACCTCTTGGGACACTGCCCAC5120.45220094679573236No Hit
CAGGAAACAGCTATGACCAAACTATTAATACCTGCAGCAAAGCCCCAAGC5060.4469017169504699No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT5000.44160248710520733No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT4660.4115735179820533No Hit
CAGGAAACAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC4600.4062742881367908No Hit
CAGGAAACAGCTATGACCTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC4530.40009185331731795No Hit
CAGGAAACAGCTATGACCTGGAAACCCATGCCATGCACTCAACACTACAA4500.3974422383946867No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCTGTCCAGCCCC4440.39214300854942413No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA4430.3912598035752137No Hit
CAGGAAACAGCTATGACCCCCTGCGGCTCCTTTCAAGTTAAGTCACCACT4320.38154454885889916No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGCTCCTCCTCGC4270.3771285239878471No Hit
CAGGAAACAGCTATGACCGGGTCCCACCCATGCTGAAATGTGATCCTCAA4210.3718292941425846No Hit
CAGGAAACAGCTATGACCCATCAGGCCCCACCTCCAACCCCGTTAGCTGC4140.3656468593231117No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCACCCCACCCTGCT4100.36211403942627No Hit
CAGGAAACAGCTATGACCGTGCTCCTTGGGGACGTAGTCGCCTGGGGACT3980.3515155797357451No Hit
CAGGAAACAGCTATGACCGCATCCTGCTTAGTGCTGCCCACAGTGTCATT3980.3515155797357451No Hit
CAGGAAACAGCTATGACCGGGTCCCACCCATGCTGAAAGTCCACCTTCCA3970.35063237476153464No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG3970.35063237476153464No Hit
CAGGAAACAGCTATGACCCAGGGTGACTCCGATCCTGGAGCCAACGGTGC3630.3206034056383806No Hit
CAGGAAACAGCTATGACCAGGGCAAGTGTTCGCGTTGTGACAGTTACGGT3560.3144209708189077No Hit
CAGGAAACAGCTATGACCGGGTCCCACCCATGCTGAAATGCGATCCTCAA3500.30912174097364514No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAATGAAACTTGGTT3410.30117289620575144No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGTGCACCTCCTTC3250.2870416166183848No Hit
CAGGAAACAGCTATGACCCTTGGTGGAGCCATAGGCCGCATGCACGCGCA3240.2861584116441744No Hit
CAGGAAACAGCTATGACCTGACAGCACAAGGAACACTGACCTACAGATAA3190.2817423867731223No Hit
CAGGAAACAGCTATGACCACCTGGCAGCCCCAAATCTGCACCTCCCGCAG3130.2764431569278598No Hit
CAGGAAACAGCTATGACCTCCCTTAAACTTGGGATTGCTCCATTGCACTC3110.274676746979439No Hit
CAGGAAACAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGCCCATACGGC3100.2737935420052286No Hit
CAGGAAACAGCTATGACCCAGGGTGACTCCGATCCTGGAGGACAAGGCGG3070.2711439270825973No Hit
CAGGAAACAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGCCAAGACGGC3060.2702607221083869No Hit
CAGGAAACAGCTATGACCACCTGGCAGCCCCAAATCTGCACCTCCCGCGG2970.2623118773404932No Hit
CAGGAAACAGCTATGACCCACGACACCCCACCTCTTGGACACTGCCCACC2910.2570126474952307No Hit
CAGGAAACAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAGTAGTCT2850.2517134176499682No Hit
CAGGAAACAGCTATGACCGCTAATGCTTACTCTTTGCGTGAGTACTGGTT2850.2517134176499682No Hit
CAGGAAACAGCTATGACCGGTCCCGCGGGGAAAGTAAGTCCTTCCCTTGG2790.24641418780470573No Hit
CAGGAAACAGCTATGACCAGCGTCTCATTCCATAAAGGCCACCATGAAAC2770.24464777785628491No Hit
CAGGAAACAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAATAGTCT2670.23581572811418072No Hit
CAGGAAACAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGTGGAGACGGC2650.2340493181657599No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCTGGGAAAAAGGG2630.23228290821733907No Hit
CAGGAAACAGCTATGACCCTTGGTGGAGCCATAGGCCGCGTGCACGCGCA2570.22698367837207656No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGGTCCTCCTC2550.22521726842365578No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTTGTAATCCCATC2540.22433406344944534No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGCTGAATAGTTGT2510.22168444852681413No Hit
CAGGAAACAGCTATGACCGGGCATGGTGTGGGACTAGCTAATCTAGGGAC2510.22168444852681413No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCAGAGCCACC2430.21461880873313077No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGCCCCTGCCTACC2390.21108598883628912No Hit
CAGGAAACAGCTATGACCTCCAGTAAAACTGCCTCTGTTTCAACTCCCTT2290.202253939094185No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAGGCCCCTGTACC2290.202253939094185No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC2260.19960432417155372No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCCTCTCCCAACT2260.19960432417155372No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAGGGGACATGGCC2220.19607150427471207No Hit
CAGGAAACAGCTATGACCTCCACAAGGCCCTGGGACAATCTCCCAGGCCC2140.18900586448102877No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGGCTGCTT2110.18635624955839752No Hit
CAGGAAACAGCTATGACCACCTTTACCCAAATAGCTACCACCAGTGCCTT2100.1854730445841871No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC2070.18282342966155585No Hit
CAGGAAACAGCTATGACCTGACTTTTGCAAGTCACAGAGAACTGTTGTAT2060.18194022468734544No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCAGGAAACAGCT2020.1784074047905038No Hit
CAGGAAACAGCTATGACCGGGGAATGGGAAGGGAGATGAAAGAGTAGGTG2000.17664099484208295No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATTGC1990.17575778986787255No Hit
CAGGAAACAGCTATGACCCTCACCAAGAGTGCCCCACGACACCCAAGACA1950.17222496997103087No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCGTGTTCTCACTC1950.17222496997103087No Hit
CAGGAAACAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGCCGAGACGGC1940.17134176499682047No Hit
CAGGAAACAGCTATGACCAGACAGGGTCTTGCTATGTTGCCCAGGCTGGT1870.16515933017734755No Hit
CAGGAAACAGCTATGACCATCGCACCACTGCACTCCAGCGCTCCATACAC1830.1616265102805059No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCAAGTGCTGATGTA1830.1616265102805059No Hit
CAGGAAACAGCTATGACCACCTGGCAGCCCCAAATCTGCGGGTGACCACG1820.16074330530629546No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT1800.15897689535787465No Hit
CAGGAAACAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGCCCAGACGGC1780.15721048540945384No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGTGACACA1760.155444075461033No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCGCCCAACACCC1760.155444075461033No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC1710.15102805058998092No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1700.1501448456157705No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAATTTCATTCT1700.1501448456157705No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACCACCGTGATCTCTC1680.14837843566734968No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAATTCCATTCT1670.14749523069313927No Hit
CAGGAAACAGCTATGACCCCCTGCGGCTCCTTTCAAGTGCAGAGGAAGCC1560.1377799759768247No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTGCAGTGAACTGA1560.1377799759768247No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGCTCAAGG1560.1377799759768247No Hit
CAGGAAACAGCTATGACCCTCACCAAGAGTGCCCCACGTGGTGGGCCACA1540.13601356602840387No Hit
CAGGAAACAGCTATGACCCTCACCAAGAGTGCCCCACGCCCAAGACACTG1450.12806472126051016No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCCGCTCAGCTCC1390.12276549141524765No Hit
CAGGAAACAGCTATGACCTCCCTGCTTGATAATGACCCCTCTGAATTCTT1360.1201158764926164No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGATACTACTA1350.11923267151840598No Hit
CAGGAAACAGCTATGACCAGACAGGGTCTTGCTATGTTGCCCAGGCTAGT1340.11834946654419559No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTACTCCCAACCA1340.11834946654419559No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCTGGGTGCTGGTG1320.11658305659577475No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTTGTAGTCCCAGC1300.11481664664735391No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCACGGTGGCTCAT1280.11305023669893309No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGCCCCACGTTCCA1270.11216703172472267No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTATTCCCAACCA1250.11040062177630183No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG1240.10951741680209143No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAACTGGCCAGCCTG1230.10863421182788101No Hit
CAGGAAACAGCTATGACCCATCAGGCCCCACCTCCAACCAGGAAACAGCT1170.10333498198261852No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCAGAGAGTGATAT1160.10245177700840812No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGCACCCCCCCATC1150.1015685720341977No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)