FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003246700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38145
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNCGACATATCCGAGCCTCACGTTCTAGTTTCTG4911.2871936033556166No Hit
NNGGANNNNGNTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG3510.9201730239874164No Hit
NAGGANNNNGNTATGNNNCGACATATCCGAGCCTCACGTTCTAGTTTCTG1990.5216935378162275No Hit
NNGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1620.424695241840346No Hit
NAGGANNNNGNTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG1290.3381832481321274No Hit
NNGGANNNNGNTATGNNNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1210.3172106435968017No Hit
NNGGANNNNGNTATGNNNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC1110.2909948879276445No Hit
NNGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC1080.2831301612268974No Hit
NNGGANNNNGNTATGNNNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1050.2752654345261502No Hit
NNGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1040.2726438589592345No Hit
NNGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA940.24642810329007733No Hit
NNGGANNNNGNTATGNNNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT930.24380652772316164No Hit
NNGGANNNNGNTATGNNNGTCTCAGGGATCACGTGGCGCAGACCTCGCTC830.21759077205400446No Hit
NNGGANNNNGNTATGNNNACAGTGTCCCCATCAGGCTCCGGGACAGCATC830.21759077205400446No Hit
NNGGANNNNGNTATGNNNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT810.212347620920173No Hit
NAGGANNNNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG800.2097260453532573No Hit
NAGGANNNAGNTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG770.20186131865251017No Hit
NNGGANNNNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC760.19923974308559445No Hit
NNGGANNNNGNTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT740.193996591951763No Hit
NAGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT730.19137501638484727No Hit
NNGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG690.18088871411718443No Hit
NNGGANNNNGNTATGNNNCCGTAGCCACGCCCCTATTGTCTGACCACGCT690.18088871411718443No Hit
NNGGANNNNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT680.1782671385502687No Hit
NNGGANNNNGNTATGNNNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG680.1782671385502687No Hit
CAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG670.17564556298335302No Hit
NNGGANNNNGNTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT660.1730239874164373No Hit
NNGGANNNNGNTATGNNNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC650.17040241184952154No Hit
NNGGANNNNGNTATGNNNGGGACCAGTCCAATAACCCCTGAGGGGTATAT640.16778083628260584No Hit
NAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG630.16515926071569012No Hit
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT610.1599161095818587No Hit
NNGGANNNNGNTATGNNNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG600.15729453401494298No Hit
NNGGANNNNGNTATGANNCCGTAGCCACGCCCCTATTGTCTGACCACGCT600.15729453401494298No Hit
NAGGAANNNGNTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG600.15729453401494298No Hit
NNGGANNNNGNTATGNNNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA600.15729453401494298No Hit
NAGGAAANAGNTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG590.15467295844802728No Hit
NNGGANNNNGNTATGNNNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA580.15205138288111156No Hit
NNGGANNNNGNNATGNNNCGACATATCCGAGCCTCACGTTCTAGTTTCTG570.14942980731419583No Hit
NNGGANNNNGNTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA560.1468082317472801No Hit
NNGGANNNNGNTATGNNNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG550.1441866561803644No Hit
NAGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC550.1441866561803644No Hit
NNGGANNNNGNTATGNNNGGGGAGACTGGGCCATACGATAGGCTTAATCC540.1415650806134487No Hit
NNGGANNNNGNTATGNNNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT530.13894350504653297No Hit
NNGGANNNNGNTATGNNNAACACGGCAGTCAGAGGTGCAGGAAACAGCTA520.13632192947961724No Hit
NNGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC520.13632192947961724No Hit
NNGGANNNNGNTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT520.13632192947961724No Hit
NAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT510.13370035391270155No Hit
NNGNNNNNNGNTATGNNNCGACATATCCGAGCCTCACGTTCTAGTTTCTG500.13107877834578582No Hit
NNGGAAANNGNTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG490.1284572027788701No Hit
NNGGANNNNGNTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG490.1284572027788701No Hit
NNGGANNNNGNTATGNNNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA490.1284572027788701No Hit
NAGGAANNNGNTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT480.12583562721195438No Hit
NAGGANNNNGNTATGNNNTGTTTTGCCCCTGAAATGAATATTCATCGTCT470.12321405164503867No Hit
NAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT470.12321405164503867No Hit
NAGGAANNNGNTATGANNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC470.12321405164503867No Hit
NNGGANNNNGNTATGNNNGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC470.12321405164503867No Hit
NNGGAAANNGNTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT460.12059247607812296No Hit
NNGGANNNNGNTATGNNNGCTGCTGACCCAACCCTACCTCTGCAGCTAGC460.12059247607812296No Hit
NNGGANANAGNTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG460.12059247607812296No Hit
NNGGANNNNGNTATGANNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC460.12059247607812296No Hit
NNGGANNNNGNTATGNNNAGGACCACCCCTCCATCCTGCTGCCTGGCTTG450.11797090051120723No Hit
NNGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG440.11534932494429151No Hit
NNGGAAANNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT440.11534932494429151No Hit
NNGGANNNNGNTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC440.11534932494429151No Hit
NNGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG430.11272774937737581No Hit
NAGGANNNNGNTATGNNNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT430.11272774937737581No Hit
NNGGANNNNGNTATGNNNTCCTGGAACCTGCCTCCGAAGGCCCCATCCGG430.11272774937737581No Hit
NNGGANNNNGNTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA430.11272774937737581No Hit
NNGGANNNNGNTATGANNGTCTCAGGGATCACGTGGCGCAGACCTCGCTC420.11010617381046009No Hit
NNGGAAANNGNTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA420.11010617381046009No Hit
NNGGANNNNGNTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA420.11010617381046009No Hit
NAGGANNNNGNTATGNNNGTCTCAGGGATCACGTGGCGCAGACCTCGCTC420.11010617381046009No Hit
NAGGAANNNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT420.11010617381046009No Hit
NAGGAANNNGNTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA410.10748459824354437No Hit
CAGGAAANAGCTATGANNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC400.10486302267662866No Hit
NNGGANNNNGNTATGNNNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC400.10486302267662866No Hit
NNGGAAANNGNTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT400.10486302267662866No Hit
NAGGAAANAGCTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT400.10486302267662866No Hit
NNGGANNNNGNTATGNNNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA390.10224144710971293No Hit
CAGGAAANAGCTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA390.10224144710971293No Hit
NNGGANNNNGNTATGANNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT390.10224144710971293No Hit
NAGGAAANAGCTATGANNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC390.10224144710971293No Hit
NNGGANNNNGNTATGNNNTCCTGGAACCTGCCTCCGAAGGCCGCAGCTCC390.10224144710971293No Hit
NAGGAANNNGNTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA390.10224144710971293No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)