FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85318
Sequences flagged as poor quality0
Sequence length300
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG38404.500808739070301No Hit
CAGGAAANAGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC15501.816732694155981No Hit
CAGGAAANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG13101.5354321479640873No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC10311.2084202630160106No Hit
CAGGAAANAGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG9741.1416113832954358No Hit
CAGGAAANAGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA8370.9810356548442298No Hit
CAGGAAANAGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT8250.9669706275346351No Hit
CAGGAAANAGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT7890.9247755456058511No Hit
CAGGAAANAGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC7090.8310086968752197No Hit
CAGGAAANNGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC6220.7290372488806582No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT6100.7149722215710635No Hit
CAGGAAANAGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG5870.6880142525610071No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA5670.6645725403783492No Hit
CAGGAAANAGCTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT4570.5356431233737312No Hit
CAGGAAANAGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG4510.5286106097189338No Hit
CAGGAAANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG4450.5215780960641365No Hit
CAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC4400.5157176680184721No Hit
CAGGAAANNGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT4250.4981363838814787No Hit
CAGGAAANAGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC4170.48875969900841554No Hit
CAGGAAANAGCTATGANNCAGGGTGACTCCGATCCTGGAGGACAAGGCGG4040.473522586089688No Hit
NAGGAAANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG3860.4524250451252959No Hit
CAGGAAANAGCTATGANNCCCAAACCTGCGGAGGCACGGGGATGGCAGTG3850.45125295951616307No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA3810.44656461707963147No Hit
NAGGANANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG3750.4395321034248342No Hit
CAGGAAANAGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA3750.4395321034248342No Hit
CAGGAAANAGCTATGANNGGGGAGACTGGGCCATACGATAGGCTTAATCC3730.4371879322065684No Hit
CAGGAAANAGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA3650.42781124733350523No Hit
CAGGAAANAGCTATGANNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG3530.41374622002391054No Hit
CAGGAAANNGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA3500.41022996319651184No Hit
CAGGANANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG3480.4078857919782461No Hit
CAGGAAANAGCTATGANNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT3430.4020253639325817No Hit
CAGGAAANNGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT3430.4020253639325817No Hit
CAGGAAANNGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC3290.38561616540472116No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA3240.3797557373590567No Hit
CAGGAAANAGCTATGANNTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG3180.37272322370425937No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA2950.3457652546942029No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC2690.3152910288567477No Hit
CAGGAAANAGCTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA2680.3141189432476148No Hit
CAGGAAANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA2680.3141189432476148No Hit
CAGGAAANNGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT2640.30943060081108326No Hit
CAGGAAANNGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG2330.27309594692796363No Hit
CAGGAAANAGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC2320.27192386131883073No Hit
CAGGAAANNGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG2270.2660634332731663No Hit
NAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG2190.25668674840010314No Hit
CAGGAAACNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG2160.25317049157270444No Hit
CAGGAAACAGCNATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG2150.2519984059635716No Hit
CAGGAAANAGCTATGANNCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC2140.2508263203544387No Hit
CAGGAAANAGCTATGANNCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT2100.24613797791790712No Hit
CAGGAAACAGCTATGANCCGACATATCCGAGCCTCACGTTCTAGTTTCTG2060.24144963548137555No Hit
CAGGAAANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC1980.23207295060831243No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC1970.23090086499917956No Hit
CAGGAANNNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG1880.2203520945169835No Hit
NAGGAAANAGCTATGANCCGACATATCCGAGCCTCACGTTCTAGTTTCTG1850.21683583768958484No Hit
CAGGAAANNGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1840.21566375208045196No Hit
CAGGAAANAGCTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA1820.2133195808621862No Hit
CAGGAAANAGCTATGANNACCTGGCAGCCCCAAATCTGCACCTCCCGCGG1790.2098033240347875No Hit
CAGGAAANNGCTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT1780.20863123842565462No Hit
CAGGAAANNGCTATGANNCAGGGTGACTCCGATCCTGGAGGACAAGGCGG1770.20745915281652172No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATTGC1700.1992545535525915No Hit
CAGGAAANAGCTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA1680.1969103823343257No Hit
CAGGAAANNGCTATGANNGGGGAGACTGGGCCATACGATAGGCTTAATCC1670.1957382967251928No Hit
CAGGAAANAGCTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC1670.1957382967251928No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC1650.19339412550692703No Hit
CAGGAAANAGCTATGANNTTGGACAGCACCTTGAATGCTTTCAGGGAAGC1600.18753369746126256No Hit
CAGGAAANNGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA1590.18636161185212968No Hit
CAGGAAANAGCTATGANNTGACAGCACAAGGAACACTGACCGTCGTTTTA1570.18401744063386388No Hit
CAGGAAANNGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1570.18401744063386388No Hit
CAGGAAANNGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA1550.1816732694155981No Hit
CAGGAAANNGCTATGANNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT1480.17346867015166786No Hit
CAGGAAANNGCTATGANNCCCAAACCTGCGGAGGCACGGGGATGGCAGTG1430.16760824210600342No Hit
CAGGAAANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA1400.16409198527860475No Hit
NAGGAAANNGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC1370.16057572845120607No Hit
CAGGANANNGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC1360.1594036428420732No Hit
CAGGAAANAGCTATGANNTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC1360.1594036428420732No Hit
NAGGANANNGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC1350.1582315572329403No Hit
CAGGAAANNGCTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA1340.1570594716238074No Hit
CAGGAAANNGCTATGANNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG1340.1570594716238074No Hit
CAGGAAANAGCTATGANNCCTGACCCCTTCTTCTCCAGGATGTAAAACTT1300.15237112918727586No Hit
CAGGAAANAGCTATGANNAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT1300.15237112918727586No Hit
CAGGAAANAGCTATGANNACCTGGCAGCCCCAAATCTGCACCTCCCGCAG1300.15237112918727586No Hit
CAGGAAANNGCTATGANNTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG1290.15119904357814296No Hit
CAGGAAANAGCTATGANNTGCCCAGTTGTTTCCAGAACCTTGTTTTTGAC1270.14885487235987718No Hit
CAGGAAACAGCTATGANCCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC1250.14651070114161138No Hit
CAGGAAANAGCTATGANNGCAAAACACCTTAACACTCGGCTTCTATTTGC1230.1441665299233456No Hit
CAGGAAANAGCTATGANNTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC1220.1429944443142127No Hit
CAGGAAANAGCTATGANNTTCATACAGAGATACAAAGGCAACTATGTGCA1200.1406502730959469No Hit
CAGGAAACAGCTATGANCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT1190.13947818748681404No Hit
CAGGAAANAGCTATGANNTGCTTCAGTCGCCTGCACAACAGGAAACAGCA1180.13830610187768114No Hit
CAGGAAANNGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC1170.13713401626854824No Hit
CAGGAAANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC1160.13596193065941536No Hit
CAGGAAACNGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC1140.13361775944114956No Hit
CAGGAANNNGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC1090.12775733139548512No Hit
CAGGAAANAGCTATGANNAATGCAAGGGACCACCAGGCACGGTGGCTCAT1080.12658524578635222No Hit
CAGGAAANAGCTATGANNCATGTGCCCCAGTAGAGAGCAATTAAATTCCT1070.12541316017721935No Hit
CAGGAAANAGCTATGANNTGAAGTGGCTCCATCCTTCCATTTGTTTATAT1070.12541316017721935No Hit
CAGGAAACAGCTATGANCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1010.118380646522422No Hit
CAGGAAANAGCTATGANNCACGACACCCCACCTCTTGGACACTGCCCACC1000.1172085609132891No Hit
CAGGAAANNGCTATGANNCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC990.11603647530415621No Hit
CAGGAAACNGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT970.11369230408589043No Hit
CAGGAAACAGCTATGANCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG950.11134813286762466No Hit
CAGGAAANAGCTATGANNGCTTCTCGGCCATGTGGGTTAAACTCGGGGAT950.11134813286762466No Hit
CAGGAAANAGCTATGANNTTTGTCGCCCTGGCTTGTCCTGTGTAAGGCGC930.10900396164935887No Hit
CAGGAAACNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC880.10314353360369442No Hit
CAGGAAANNGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC870.10197144799456152No Hit
NAGGAAANAGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC870.10197144799456152No Hit
CAGGAAACAGCTATGANCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC870.10197144799456152No Hit
CAGGAAACNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG860.10079936238542864No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)