FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68987
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT3380.4899473813906968No Hit
NNGGANNNNGNTATGNNNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT2850.4131213127110905No Hit
NNGGANNNNGNTATGNNNTGGAAACCCATGCCATGCACTCAACACTACAA2540.36818531027584905No Hit
NNGGANNNNGNTATGNNNTGGAGTTTGAAACAGCACCTTGGCACTCCTTC2490.36093756794758436No Hit
NNGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAACT2400.3478916317567078No Hit
NNGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC2370.34354298635974895No Hit
NNGGANNNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT2280.3304970501688724No Hit
NNGGANNNNGNTATGANNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT2040.2957078869932016No Hit
NNGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAATT1970.28556104773363095No Hit
NAGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1970.28556104773363095No Hit
NNGGANNNNGNTATGNNNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA1930.2797628538710192No Hit
NNGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA1880.27251511154275443No Hit
NNGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC1850.2681664661457956No Hit
NNGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT1840.26671691768014266No Hit
NNGGANNNNGNTATGNNNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1710.2478727876266543No Hit
NNGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT1700.24642323916100134No Hit
NNGGANNNNGNTATGNNNTGCTGGGAGAGGGGGATGAGTAGACAGGGCCA1690.2449736906953484No Hit
NNGGANNNNGNTATGNNNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT1660.24062504529838957No Hit
NNGGANNNNGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA1660.24062504529838957No Hit
NNGGANNNNGNTATGNNNTCCCTTAAACTTGGGATTGCTCCATTGCACTC1640.23772594836708366No Hit
NNGGANNNNGNTATGNNNGGTAGCAACGTCCCACCCAGGGCACGGGAACA1640.23772594836708366No Hit
NNGGANNNNGNTATGNNNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC1590.2304782060388189No Hit
NAGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC1570.22757910910751303No Hit
NNGGANNNNGNTATGANNTGGAGTTTGAAACAGCACCTTGGCACTCCTTC1540.22323046371055416No Hit
NNGGANNNNGNTATGNNNAGGCACCAACAGTACAAAGCAGATACAAAGCA1490.21598272138228944No Hit
NAGGANNNNGNTATGNNNTGGAGTTTGAAACAGCACCTTGGCACTCCTTC1480.21453317291663648No Hit
NAGGANNNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1440.20873497905402466No Hit
NNGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT1430.2072854305883717No Hit
NNGGANNNNGNTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1430.2072854305883717No Hit
NAGGANNNNGNTATGNNNTGGAAACCCATGCCATGCACTCAACACTACAA1380.20003768826010698No Hit
NNGGANNNNGNTATGNNNGGGACCAGTCCAATAACCCCTGAGGGGTATAT1380.20003768826010698No Hit
NNGGANNNNGNTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA1340.1942394943974952No Hit
NAGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAACT1300.1884413005348834No Hit
NNGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1280.18554220360357748No Hit
NNGGANNNNGNTATGNNNCAGGGCTCCAAGAATGTTCCACACATCTTTCT1260.18264310667227157No Hit
NNGGANNNNGNTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA1260.18264310667227157No Hit
NNGGANNNNGNTATGNNNCATGGTTGGGGAAAAACTCCTCATCTTCATGT1240.1797440097409657No Hit
NAGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAATT1210.17539536434400685No Hit
NAGGANNNNGNTATGNNNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT1200.1739458158783539No Hit
NNGGANNNNGNTATGANNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT1200.1739458158783539No Hit
NNGGANNNNGNTATGNNNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT1200.1739458158783539No Hit
NNGGANNNNGNTATGNNNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1180.17104671894704798No Hit
NNGGANNNNGNTATGNNNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1170.16959717048139503No Hit
NNGGANNNNGNTATGNNNCATGGTTGGGGAAAAACTCCTCATTTTCATGT1150.16669807355008914No Hit
NNGGANNNNGNTATGNNNGGGACCAGTCCAATAACCCCTGACAGGAAACA1150.16669807355008914No Hit
NNGGANNNNGNTATGANNTCCCTTAAACTTGGGATTGCTCCATTGCACTC1130.16379897661878323No Hit
NNGGANNNNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1120.16234942815313028No Hit
NNGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA1100.1594503312218244No Hit
NAGGAANNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1060.1536521373592126No Hit
NAGGANNNNGNTATGNNNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT1050.15220258889355964No Hit
NAGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT1020.1478539434966008No Hit
NNGGANNNNGNTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT1020.1478539434966008No Hit
NAGGANNNNGNTATGNNNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA1020.1478539434966008No Hit
NNGGANNNNGNTATGANNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC1020.1478539434966008No Hit
NAGGAAANAGCTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1010.14640439503094788No Hit
NNGGANNNNGNTATGANNGGTAGCAACGTCCCACCCAGGGCACGGGAACA1000.14495484656529492No Hit
NNGGANNNNGNTATGNNNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG1000.14495484656529492No Hit
NNGGANNNNGNTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG990.14350529809964196No Hit
NNGGANNNNGNTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA990.14350529809964196No Hit
CAGGAAANAGCTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT980.142055749633989No Hit
NNGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA970.14060620116833608No Hit
NNGGANNNNGNTATGANNTGCTGGGAGAGGGGGATGAGTAGACAGGGCCA970.14060620116833608No Hit
NAGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC960.13915665270268313No Hit
NAGGANNNNGNTATGNNNAGGCACCAACAGTACAAAGCAGATACAAAGCA960.13915665270268313No Hit
NNGGANNNNGNTATGANNCATGGTTGGGGAAAAACTCCTCATCTTCATGT960.13915665270268313No Hit
NAGGANNNNGNTATGNNNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA960.13915665270268313No Hit
NNGGAAANNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC940.13625755577137721No Hit
NAGGANNNNGNTATGANNTGGAGTTTGAAACAGCACCTTGGCACTCCTTC940.13625755577137721No Hit
NAGGANNNNGNTATGNNNTCCCTTAAACTTGGGATTGCTCCATTGCACTC930.1348080073057243No Hit
NNGGAAANNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT930.1348080073057243No Hit
NNGGANNNNGNTATGNNNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA920.13335845884007133No Hit
NNGGANNNNGNTATGNNNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA900.13045936190876542No Hit
NNGGANNNNGNTATGNNNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA900.13045936190876542No Hit
NNGGANNNNGNTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT900.13045936190876542No Hit
NAGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT900.13045936190876542No Hit
NAGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA900.13045936190876542No Hit
NNGGANNNNGNTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA890.1290098134431125No Hit
NNGGANNNNGNTATGNNNTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC890.1290098134431125No Hit
NAGGANNNNGNTATGNNNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC890.1290098134431125No Hit
NAGGAAANAGCTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA890.1290098134431125No Hit
NAGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC880.12756026497745954No Hit
NNGGANNNNGNTATGANNCATGGTTGGGGAAAAACTCCTCATTTTCATGT870.12611071651180658No Hit
NAGGANNNNGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA860.12466116804615363No Hit
NAGGAANNNGNTATGANNTGGAGTTTGAAACAGCACCTTGGCACTCCTTC860.12466116804615363No Hit
NAGGANNNNGNTATGNNNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG860.12466116804615363No Hit
NAGGAAANAGCTATGANNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT850.12321161958050067No Hit
NAGGAAANAGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT840.12176207111484773No Hit
NNGGANNNNGNTATGNNNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT830.12031252264919479No Hit
NNGGANNNNGNTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT830.12031252264919479No Hit
NAGGAAANAGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT820.11886297418354183No Hit
NAGGANNNNGNTATGANNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT820.11886297418354183No Hit
NNGGANNNNGNTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG810.11741342571788888No Hit
NAGGANNNNGNTATGNNNCAGGGCTCCAAGAATGTTCCACACATCTTTCT800.11596387725223592No Hit
NAGGANNNNGNTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA800.11596387725223592No Hit
CAGGAAANAGCTATGANNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT790.11451432878658299No Hit
NAGGANNNNGNTATGNNNTGCTGGGAGAGGGGGATGAGTAGACAGGGCCA780.11306478032093004No Hit
NAGGANNNNGNTATGNNNGGTAGCAACGTCCCACCCAGGGCACGGGAACA780.11306478032093004No Hit
NAGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA770.11161523185527708No Hit
NNGGANNNNGNTATGNNNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA770.11161523185527708No Hit
NAGGAAANAGCTATGANNTGGAGTTTGAAACAGCACCTTGGCACTCCTTC760.11016568338962412No Hit
NAGGANNNNGNTATGNNNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA760.11016568338962412No Hit
NNGGAAANNGNTATGANNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT760.11016568338962412No Hit
NNGGAAANNGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA760.11016568338962412No Hit
CAGGAAANAGCTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC760.11016568338962412No Hit
NAGGAANNNGNTATGANNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT750.1087161349239712No Hit
NNGGANNNNGNTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT740.10726658645831824No Hit
NNGGAAANNGNTATGANNTGGAGTTTGAAACAGCACCTTGGCACTCCTTC740.10726658645831824No Hit
NAGGAANNNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC730.10581703799266529No Hit
NNGGANNNNGNTATGANNGGGACCAGTCCAATAACCCCTGACAGGAAACA730.10581703799266529No Hit
NNGGANNNNGNTATGNNNCATGTGCCCCAGTAGAGAGCAACCATGTGCCC730.10581703799266529No Hit
NNGGANNNNGNTATGNNNAAGGACAAGGTGAGGCAGCACCCCACCCTGCT720.10436748952701233No Hit
NNGGANNNNGNTATGNNNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT720.10436748952701233No Hit
CAGGAAANAGCTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA710.10291794106135937No Hit
NNGGANNNNGNTATGNNNAACACGGCAGTCAGAGGTGCAGGAAACAGCTA710.10291794106135937No Hit
NAGGANNNNGNTATGNNNGGGACCAGTCCAATAACCCCTGAGGGGTATAT710.10291794106135937No Hit
NAGGAAANAGCTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC700.10146839259570645No Hit
NAGGAANNNGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA690.10001884413005349No Hit
NNGGANNNNGNTATGNNNCTTGAGCTCCTCGGACACGGCAGGAAACAGCT690.10001884413005349No Hit
NAGGANNNNGNTATGNNNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA690.10001884413005349No Hit
NAGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT690.10001884413005349No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)