FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003246710

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183654
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA34491.877987955612184No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA33441.8208152286364576No Hit
CAGGAAACAGCTATGACCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT32991.7963126313611466No Hit
CAGGAAACAGCTATGACCCAGGGCTCCAAGAATGTTCCACACATCTTTCT30251.6471190390625852No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT28941.5757892558833457No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT28521.5529201650930553No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC28441.5485641477996668No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC27991.5240615505243555No Hit
CAGGAAACAGCTATGACCCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT26641.450553758698422No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA26581.4472867457283805No Hit
CAGGAAACAGCTATGACCGCTTCTATATGAGCGCATCTCACCTGAATTTG25951.4129831095429448No Hit
CAGGAAACAGCTATGACCGTCCTAGCACCGCAGCGAATGGGACCCGCAAG23751.2931926339747568No Hit
CAGGAAACAGCTATGACCAACCAACACTGCTGCTAATTGTGTTTGTGTCC23011.2528994740109118No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG22511.2256743659272327No Hit
CAGGAAACAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCATA21881.191370729741797No Hit
CAGGAAACAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA20721.1282084789876614No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT20721.1282084789876614No Hit
CAGGAAACAGCTATGACCGCCCAGCAATGGAGAGGCTACCAGCTGATCCA19591.0666797347185468No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGCACTCCTTC18881.0280200812397227No Hit
CAGGAAACAGCTATGACCGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC18050.9828264018208152No Hit
CAGGAAACAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG16340.8897165321746328No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT15440.8407113376240104No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGGCTGTTT14830.8074967057619219No Hit
CAGGAAACAGCTATGACCGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA14830.8074967057619219No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACCAGGGCAGCGGT14790.8053186971152275No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCTGCCCAACACCC14630.7966066625284502No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACCCTTTGTCCA14070.7661145414747297No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT13780.7503239787861958No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC13720.7470569658161543No Hit
CAGGAAACAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT13660.7437899528461128No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT13150.7160203426007601No Hit
CAGGAAACAGCTATGACCTGGCCCCAGTGTTGAGAGGTTCTTTTATTCCG12600.6860727237087132No Hit
CAGGAAACAGCTATGACCGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC12580.684983719385366No Hit
CAGGAAACAGCTATGACCTCCTCTTCATTTTCACGGTGCCATTCTGATTC12330.6713711653435264No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT12190.6637481350800962No Hit
CAGGAAACAGCTATGACCGCAGTGACAGGGCAAGGAGGGTGGATGGATCC12010.6539470961699717No Hit
CAGGAAACAGCTATGACCGGCGGGCCCTGATATAGAGCCACTGGCCGTCG11740.6392455378047851No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGCGGCCTTGCTGA11730.6387010356431115No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC11550.628899996732987No Hit
CAGGAAACAGCTATGACCTCTGACTGTCCGTGATCCCACACAATCACCCT10570.575538784888976No Hit
CAGGAAACAGCTATGACCGCCCCTACTCCAAAAGGCGGATGAATGGGGAA10350.5635597373321573No Hit
CAGGAAACAGCTATGACCGGGTCCCACCCATGCTGAAATGTGATCCTCAA10150.5526696940986855No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA10100.5499471832903177No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTCCCCAACCATAG10080.5488581789669705No Hit
CAGGAAACAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC9970.5428686551885611No Hit
CAGGAAACAGCTATGACCAGATCCTGTGAAGTGGGAGGGTTTTCAAAGTC9680.5270780925000272No Hit
CAGGAAACAGCTATGACCGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG9540.519455062236597No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCTGTCCAGCCCC9500.5172770535899027No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACCCTGTCTCTCTAGC9450.5145545427815349No Hit
CAGGAAACAGCTATGACCAAAACCAAGTCACCTGTTGATCTGTGCAGGAA9430.5134655384581877No Hit
CAGGAAACAGCTATGACCGGGTCCCACCCATGCTGAAAGTCCACCTTCCA9330.5080205168414519No Hit
CAGGAAACAGCTATGACCGTGCTCCTTGGGGACGTAGTCGCCTGGGGACT9270.5047535038714104No Hit
CAGGAAACAGCTATGACCTCCCTTAAACTTGGGATTGCTCCATTGCACTC9170.4993084822546745No Hit
CAGGAAACAGCTATGACCGCTCCTGTGCTGTCAAGGGACTCCTGTAGTGG9150.4982194779313274No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAATGAAACTTGGTT9030.49168545199124436No Hit
CAGGAAACAGCTATGACCTGCTGGGAGAGGGGGATGAGCGGCGCCGCCAT8450.46010432661417666No Hit
CAGGAAACAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC8040.43777973798555975No Hit
CAGGAAACAGCTATGACCTCCAGCTGCTCCATCTTGTCTACCTCCTGGAA8000.4356017293388655No Hit
CAGGAAACAGCTATGACCCCTGGACGCAATCACCCAGGCCAGCTAAGTGA7810.42525618826706746No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGATTCTTTT7500.4083766212551864No Hit
CAGGAAACAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC7490.4078321190935128No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC7420.40402060396179773No Hit
CAGGAAACAGCTATGACCAAAACCAAGTCACCTGTTGATCTGTCAGGAAA7340.3996645866684091No Hit
CAGGAAACAGCTATGACCGCTAATGCTTACTCTTTGCGTGAGTACTGGTT7280.3963975736983676No Hit
CAGGAAACAGCTATGACCTGACAGCACAAGGAACACTGACCTACAGATAA6610.3599159288662376No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT6600.359371426704564No Hit
CAGGAAACAGCTATGACCACCGTTAAGCCACACCAAATGGTGAGCCCTGC6530.35555991157284894No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGCTGAATAGTTGT6510.3544709072495018No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCTGGGAAAAAGGG6250.34031385104598866No Hit
CAGGAAACAGCTATGACCAGCAAAGGTCTTGGCAGGGGTTCTCCTTCCCT6160.3354133315909264No Hit
CAGGAAACAGCTATGACCTGACTGCAAACATAGAAGTGAGCAGAGCAAGA6110.3326908207825585No Hit
CAGGAAACAGCTATGACCCATCAGGCCCCACCTCCAACCCCGTTAGCTGC6090.33160181645921133No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCACCCCACCCTGCT5800.3158112537706775No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGCTCCTCCTCGC5770.3141777472856567No Hit
CAGGAAACAGCTATGACCCACCGCTGTCCCTTCCCAAGGGGCTGAAGGTG5610.3054657126988794No Hit
CAGGAAACAGCTATGACCACTCCCTTGAGCCTTTGGGGAAAGGGAGACAG5590.3043767083755322No Hit
CAGGAAACAGCTATGACCAGCGTCTCATTCCATAAAGGCCACCATGAAAC5550.30219869972883795No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAGGCACTACGT5190.282596621908589No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTTGCAGTGAGCCG5150.28041861326189466No Hit
CAGGAAACAGCTATGACCGGGTCCCACCCATGCTGAAATGCGATCCTCAA4880.26571705489670794No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCAGAGCCACC4780.2602720332799721No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCGTGTTCTCACTC4600.25047099436984765No Hit
CAGGAAACAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAGTAGTCT4480.24393696842976464No Hit
CAGGAAACAGCTATGACCTCCAGTAAAACTGCCTCTGTTTCAACTCCCTT4480.24393696842976464No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAGGGGACATGGCC4460.2428479641064175No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGCTGCCTGGCTTG4450.24230346194474392No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACCCGTCCTCGGAC4260.23195792087294587No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCAAGCCTGACTG4260.23195792087294587No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG4070.2216123798011478No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCAGGTCCCGGGA4020.21888986899277987No Hit
CAGGAAACAGCTATGACCATCGCACCACTGCACTCCAGCGCTCCATACAC3960.2156228560227384No Hit
CAGGAAACAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAATAGTCT3940.21453385169939126No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTTGTAATCCCATC3860.2101778344060026No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGTGACACA3810.2074553235976347No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAACGCAGCTCCCCA3680.20037679549587814No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCAAGTGCTGATGTA3590.19547627604081588No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAATTCCATTCT3530.19220926307077438No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA3510.19112025874742722No Hit
CAGGAAACAGCTATGACCCTGGGCCCCAAAGCAAAGGACTCTTCCCAGTG3380.18404173064567067No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAATTTCATTCT3240.17641870038224053No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAGGCCCCTGTACC3100.16879567011881036No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGGCTGCTT3070.16716216363378963No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCGGGTCTTACA3030.1649841549870953No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGCCAGAGGGGTC3010.16389515066374813No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACGGCCCCTCTGCC2950.16062813769370665No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT2940.16008363553203306No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTGCAGTGAACTGA2880.15681662256199158No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTACTCCCAACCA2860.1557276182386444No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAACTGGCCAGCCTG2790.15191610310692935No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGGCACGGGAACA2740.1491935922985614No Hit
CAGGAAACAGCTATGACCTCCAGCCAGCCAGTCCTTGTAACTCAGCATGA2720.14810458797521428No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAACAGCAGACACC2720.14810458797521428No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCAGGAAACAGCT2680.14592657932851993No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTATTCCCAACCA2660.14483757500517278No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCGCCCAACACCC2640.14374857068182562No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGGTCCTCCTC2620.14265956635847846No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTGATCCCCCAAAA2580.1404815577117841No Hit
CAGGAAACAGCTATGACCAGACAGGGTCTTGCTATGTTGCCCAGGCTAGT2510.13667004258006904No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAACACGCAGCTCCC2510.13667004258006904No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCTCTGCAGCTAGC2450.13340302961002756No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGCTCAAGG2430.13231402528668038No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAACAGTGAAGACAG2410.13122502096333322No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGTAGTCTC2410.13122502096333322No Hit
CAGGAAACAGCTATGACCTGCTGGGAGAGGGGGATGAGTAGACAGGGCCA2350.12795800799329174No Hit
CAGGAAACAGCTATGACCAGACAGGGTCTTGCTATGTTGCCCAGGCTGGT2290.12469099502325025No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTGTGGGGGAGCC2270.12360199069990309No Hit
CAGGAAACAGCTATGACCACTCCCTTGAGCCTTTGGGGGCCACTGCAGCT2260.1230574885382295No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCAGGAAACAGCT2240.12196848421488232No Hit
CAGGAAACAGCTATGACCGCAGCAAGAGACCAATACCGCATTGTAGTCAA2140.11652346259814653No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTTGTAGTCCCAGC2110.11488995611312576No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTCCCTTTGGGCT2110.11488995611312576No Hit
CAGGAAACAGCTATGACCACCGTTAAGCCACACCAAATGGAGCCCTGCAG2080.11325644962810504No Hit
CAGGAAACAGCTATGACCCCTGACCCCTTCTTCTCCAGGAAACTCTCTTC2050.11162294314308427No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA2020.10998943665806353No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCCTCTCCCAACT2000.10890043233471637No Hit
CAGGAAACAGCTATGACCCCTGACCCCTTCTTCTCCAGGAAACTCTGTTC1950.10617792152634847No Hit
CAGGAAACAGCTATGACCATCGCACCACTGCACTCCAGCCACTGCGCTCC1930.1050889172030013No Hit
CAGGAAACAGCTATGACCTTGGACAGCACCTTGAATGCCTGGGTTCTAAC1920.1045444150413277No Hit
CAGGAAACAGCTATGACCGCTCCTGTGCTGTCAAGGGACTCCGCCATTCA1870.10182190423295981No Hit
CAGGAAACAGCTATGACCACCCAGTTAGAGCCATGGTTCCACTAGACTCT1850.10073289990961264No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)