FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126489
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC5340.42217109788202933No Hit
NNGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT5300.41900876756081556No Hit
NNGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAATT5170.40873119401687097No Hit
NNGGANNNNGNTATGNNNACCACACCTGGCCTGAGGATGCTGTCCTGGGT5130.40556886369565737No Hit
NNGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT5110.40398769853505045No Hit
NNGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAACT4970.39291954241080257No Hit
NNGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA4910.388176046928982No Hit
NNGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT4480.35418099597593466No Hit
NNGGANNNNGNTATGNNNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT4260.3367881792092593No Hit
NNGGANNNNGNTATGNNNGGTAGCAACGTCCCACCCAGGCTCCTCCTCGC4250.33599759662895584No Hit
NNGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT4200.3320446837274387No Hit
NNGGANNNNGNTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA4120.32572002308501136No Hit
NNGGANNNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT4120.32572002308501136No Hit
NNGGANNNNGNTATGNNNAGGCACCAACAGTACAAAGCAGATACAAAGCA3870.30595545857742573No Hit
NNGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT3830.302793128256212No Hit
NNGGANNNNGNTATGNNNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA3820.30200254567590856No Hit
NNGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC3800.3004213805153017No Hit
NNGGANNNNGNTATGNNNTGGGGATGAGGACAGCAGGGCAGTCACCATCT3690.2917249721319641No Hit
NNGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT3680.2909343895516606No Hit
NNGGANNNNGNTATGANNACCACACCTGGCCTGAGGATGCTGTCCTGGGT3660.28935322439105376No Hit
NNGGANNNNGNTATGNNNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT3620.28619089406984005No Hit
NNGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG3530.2790756508471092No Hit
NNGGANNNNGNTATGANNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT3420.27037924246377154No Hit
NNGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC3280.2593110863395236No Hit
NNGGANNNNGNTATGANNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT3260.25772992117891674No Hit
NNGGANNNNGNTATGNNNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC3020.23875593925163455No Hit
NNGGANNNNGNTATGANNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG2870.22689720054708315No Hit
NNGGANNNNGNTATGNNNATTAGCCAGGTGTGGTGGCGTGCACCTCCTTC2840.2245254528061729No Hit
NNGGANNNNGNTATGANNGGTAGCAACGTCCCACCCAGGCTCCTCCTCGC2680.21187613152131804No Hit
NNGGANNNNGNTATGNNNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG2650.20950438378040778No Hit
NNGGANNNNGNTATGNNNGGGGAGACTGGGCCATACGATAGGCTTAATCC2550.20159855797737353No Hit
NNGGANNNNGNTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA2550.20159855797737353No Hit
NNGGANNNNGNTATGNNNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC2510.1984362276561598No Hit
NNGGANNNNGNTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA2500.19764564507585639No Hit
NNGGANNNNGNTATGANNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT2460.1944833147546427No Hit
NNGGANNNNGNTATGANNATTAGCCAGGTGTGGTGGCGTGCACCTCCTTC2430.19211156701373241No Hit
NNGGANNNNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2420.191320984433429No Hit
NAGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2420.191320984433429No Hit
NNGGANNNNGNTATGNNNCCCTGCGGCTCCTTTCAAGTGCAGAGGAAGCC2400.18973981927282213No Hit
NNGGANNNNGNTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT2390.18894923669251873No Hit
NNGGANNNNGNTATGNNNCATGGTTGGGGAAAAACTCCTCATCTTCATGT2360.18657748895160844No Hit
NAGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC2350.18578690637130502No Hit
NNGGANNNNGNTATGNNNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT2350.18578690637130502No Hit
NNGGANNNNGNTATGNNNTAGAGGACACCCAAGGCCACGGAGATCCAGCC2320.18341515863039476No Hit
NAGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA2320.18341515863039476No Hit
NAGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT2310.1826245760500913No Hit
NAGGANNNNGNTATGNNNACCACACCTGGCCTGAGGATGCTGTCCTGGGT2280.18025282830918102No Hit
NAGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAACT2270.17946224572887762No Hit
NAGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT2240.17709049798796733No Hit
NNGGANNNNGNTATGNNNCCCGTGACAGCTCCTGCAATTACAAAATGGCT2180.1723470025061468No Hit
NAGGANNNNGNTATGNNNATTAGCCAGGTGTGGTGGCGTGCACCTCCTTC2140.16918467218493308No Hit
NNGGANNNNGNTATGNNNGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA2120.16760350702432625No Hit
NNGGANNNNGNTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG2080.1644411767031125No Hit
NAGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG2080.1644411767031125No Hit
NAGGANNNNGNTATGNNNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT1970.15574476831977485No Hit
NNGGANNNNGNTATGNNNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA1950.154163603159168No Hit
NAGGANNNNGNTATGNNNGGTAGCAACGTCCCACCCAGGCTCCTCCTCGC1930.15258243799856114No Hit
NAGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAATT1920.1517918554182577No Hit
NNGGANNNNGNTATGNNNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1870.1478389425167406No Hit
NNGGANNNNGNTATGNNNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC1860.14704835993643717No Hit
NNGGANNNNGNTATGNNNTCCTGGAACCTGCCTCCGAAGGCCGCAGCTCC1860.14704835993643717No Hit
NNGGANNNNGNTATGANNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC1850.14625777735613374No Hit
NNGGANNNNGNTATGNNNTCCCTTAAACTTGGGATTGCTCCATTGCACTC1830.14467661219552688No Hit
NNGGANNNNGNTATGANNGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA1800.1423048644546166No Hit
NNGGANNNNGNTATGNNNGGTAGCAACGTCCCACCCAGACCTCTCCTCCT1800.1423048644546166No Hit
NNGGANNNNGNTATGNNNCATGGTTGGGGAAAAACTCCTCATTTTCATGT1790.14151428187431317No Hit
NNGGANNNNGNTATGNNNCTGGTCCTCATCCTTGGGGCCTGAGAGCCTGT1770.13993311671370634No Hit
NNGGANNNNGNTATGNNNGGTAGCAACGTCCCACCCAGGGCACGGGAACA1740.13756136897279606No Hit
NNGGANNNNGNTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT1740.13756136897279606No Hit
NNGGANNNNGNTATGNNNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1730.13677078639249263No Hit
NAGGANNNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1690.13360845607127894No Hit
NAGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC1680.13281787349097549No Hit
NNGGANNNNGNTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC1620.12807437800915494No Hit
NNGGANNNNGNTATGNNNTCCCACCACTGACTCCAGGGGAAGGAGGAGGA1610.12728379542885151No Hit
NAGGANNNNGNTATGNNNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1590.12570263026824466No Hit
NNGGANNNNGNTATGANNTCCTGGAACCTGCCTCCGAAGGCCGCAGCTCC1580.12491204768794126No Hit
NNGGANNNNGNTATGNNNCTGGTCCTCATCCTTGGGGCCGGAGAGCCTGT1550.12254029994703097No Hit
NNGGANNNNGNTATGANNCCCTGCGGCTCCTTTCAAGTGCAGAGGAAGCC1540.12174971736672753No Hit
NNGGANNNNGNTATGANNGGGGAGACTGGGCCATACGATAGGCTTAATCC1520.12016855220612069No Hit
NAGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1520.12016855220612069No Hit
NAGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1500.11858738704551383No Hit
NNGGANNNNGNTATGNNNGGTAGCAACGTCCCACCCAGCCTGCCCAGGCC1490.11779680446521042No Hit
NAGGANNNNGNTATGNNNAGGCACCAACAGTACAAAGCAGATACAAAGCA1490.11779680446521042No Hit
NNGGANNNNGNTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC1490.11779680446521042No Hit
NAGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT1490.11779680446521042No Hit
NNGGANNNNGNTATGANNCTGGTCCTCATCCTTGGGGCCTGAGAGCCTGT1480.11700622188490699No Hit
NNGGANNNNGNTATGNNNGTCTCAGGGATCACGTGGCGCAGACCTCGCTC1470.11621563930460357No Hit
NAGGANNNNGNTATGNNNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT1460.11542505672430013No Hit
NNGGANNNNGNTATGANNTAGAGGACACCCAAGGCCACGGAGATCCAGCC1450.1146344741439967No Hit
NNGGANNNNGNTATGANNCCCGTGACAGCTCCTGCAATTACAAAATGGCT1440.11384389156369329No Hit
NNGGANNNNGNTATGANNCATGGTTGGGGAAAAACTCCTCATCTTCATGT1420.11226272640308645No Hit
NNGGANNNNGNTATGNNNACACCCGGAGCAGTGCAAATCAACTCCCCCAG1420.11226272640308645No Hit
NNGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC1410.111472143822783No Hit
NAGGANNNNGNTATGANNGGTAGCAACGTCCCACCCAGGCTCCTCCTCGC1400.11068156124247959No Hit
NAGGANNNNGNTATGNNNTGGGGATGAGGACAGCAGGGCAGTCACCATCT1380.10910039608187275No Hit
NNGGANNNNGNTATGNNNCTTGGTGGAGCCATAGGCCGCGTGCACGCGCA1360.10751923092126588No Hit
NNGGANNNNGNTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1360.10751923092126588No Hit
NAGGANNNNGNTATGANNACCACACCTGGCCTGAGGATGCTGTCCTGGGT1360.10751923092126588No Hit
NNGGANNNNGNTATGNNNTGCCAAAATTAAGGATGCACACCTGTTATCCC1360.10751923092126588No Hit
NNGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT1360.10751923092126588No Hit
NNGGANNNNGNTATGNNNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA1340.10593806576065902No Hit
NAGGANNNNGNTATGANNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT1340.10593806576065902No Hit
NNGGANNNNGNTATGNNNTGCCAAAATTAAGGATGCACACCTGGAGTCAC1330.1051474831803556No Hit
NNGGANNNNGNTATGNNNGCTACGGGCAATCACTCTCCTAGTGGCATGAA1310.10356631801974876No Hit
NNGGANNNNGNTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1300.10277573543944532No Hit
NAGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT1300.10277573543944532No Hit
NAGGANNNNGNTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA1300.10277573543944532No Hit
NNGGANNNNGNTATGANNTGCCAAAATTAAGGATGCACACCTGTTATCCC1290.1019851528591419No Hit
NAGGANNNNGNTATGNNNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC1290.1019851528591419No Hit
NNGGANNNNGNTATGNNNGGGTCCCACCCATGCTGAAATGTGATCCTCAA1280.10119457027883848No Hit
NNGGANNNNGNTATGNNNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA1280.10119457027883848No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)