FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003246716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33163
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA1690.5096040768326147No Hit
NNGGANNNNGNTATGNNNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA1330.40104936224105175No Hit
NNGGANNNNGNTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA1160.34978741368392485No Hit
NNGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1080.32566414377468866No Hit
NNGGANNNNGNTATGNNNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA1020.3075716913427615No Hit
NNGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC960.2894792389108344No Hit
NNGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC940.28344842143352533No Hit
NNGGANNNNGNTATGNNNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC880.2653559690015982No Hit
NNGGANNNNGNTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA880.2653559690015982No Hit
NNGGANNNNGNTATGNNNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT870.26234056026294367No Hit
NAGGANNNNGNTATGNNNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA860.25932515152428914No Hit
NNGGANNNNGNTATGNNNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT850.2563097427856346No Hit
NNGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC800.24123269909236197No Hit
NNGGANNNNGNTATGNNNCTGGAATTTCAACAGAATGGCATTTATATGAT790.23821729035370742No Hit
NAGGANNNNGNTATGNNNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA790.23821729035370742No Hit
NNGGANNNNGNTATGNNNCAGGGCTCCAAGAATGTTCCACACATCTTTCT790.23821729035370742No Hit
NNGGANNNNGNTATGNNNAGGCACCAACAGTACAAAGCAGATACAAAGCA720.21710942918312576No Hit
NNGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGGACACTGCCCAC710.21409402044447126No Hit
NNGGANNNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC700.21107861170581674No Hit
NNGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG670.20203238548985314No Hit
NNGGANNNNGNTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA660.19901697675119862No Hit
NNGGANNNNGNTATGNNNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT650.1960015680125441No Hit
NNGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT650.1960015680125441No Hit
NNGGANNNNGNTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT640.19298615927388957No Hit
NNGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT620.18695534179658052No Hit
NNGGANNNNGNTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC620.18695534179658052No Hit
NAGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC600.18092452431927147No Hit
NNGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC590.17790911558061695No Hit
NNGGANNNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC590.17790911558061695No Hit
NNGGANNNNGNTATGNNNACACCCGGAGCAGTGCAAATCAACTCCCCCAG590.17790911558061695No Hit
NNGGANNNNGNTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT590.17790911558061695No Hit
NAGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT580.17489370684196243No Hit
NNGGANNNNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT580.17489370684196243No Hit
NNGGANNNNGNTATGNNNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG580.17489370684196243No Hit
NNGGANNNNGNTATGNNNTAGAGGACACCCAAGGCCACCAGGGCAGCGGT570.1718782981033079No Hit
NAGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG570.1718782981033079No Hit
NAGGAANNNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT570.1718782981033079No Hit
NAGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC570.1718782981033079No Hit
NNGGANNNNGNTATGNNNGCTTCTCGGCCATGTGGGTTAAACTCGGGGAT560.16886288936465338No Hit
NAGGAANNNGNTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA560.16886288936465338No Hit
NAGGANNNNGNTATGNNNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT550.16584748062599886No Hit
NNGGANNNNGNTATGNNNTGGAAACCCATGCCATGCACTCAACACTACAA530.1598166631486898No Hit
NNGGANNNNGNTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT520.15680125441003528No Hit
CAGGAAANAGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA510.15378584567138076No Hit
NAGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT510.15378584567138076No Hit
NAGGAAANAGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA510.15378584567138076No Hit
NAGGANNNNGNTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA510.15378584567138076No Hit
NAGGANNNNGNTATGNNNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT500.15077043693272624No Hit
NNGGANNNNGNTATGNNNTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC500.15077043693272624No Hit
NNGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC500.15077043693272624No Hit
NNGGANNNNGNTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC490.1477550281940717No Hit
NAGGANNNNGNTATGNNNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA480.1447396194554172No Hit
NNGGANNNNGNTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT480.1447396194554172No Hit
NNGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG470.14172421071676267No Hit
NAGGAANNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC470.14172421071676267No Hit
NNGGAAANNGNTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA470.14172421071676267No Hit
NNGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT470.14172421071676267No Hit
NNGGANNNNGNTATGNNNTCCCTTAAACTTGGGATTGCTCCATTGCACTC460.13870880197810814No Hit
NNGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC460.13870880197810814No Hit
NAGGAANNNGNTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA450.1356933932394536No Hit
NNGGANNNNGNTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT450.1356933932394536No Hit
NNGGANNNNGNTATGNNNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG450.1356933932394536No Hit
NAGGANNNNGNTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA440.1326779845007991No Hit
NAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT430.12966257576214457No Hit
NNGGANNNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT430.12966257576214457No Hit
NAGGAANNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC430.12966257576214457No Hit
NAGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGGACACTGCCCAC420.12664716702349002No Hit
NAGGANNNNGNTATGNNNCTGGAATTTCAACAGAATGGCATTTATATGAT420.12664716702349002No Hit
NAGGAAANAGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC420.12664716702349002No Hit
NNGGAAANNGNTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA410.12363175828483551No Hit
NNGGANNNNGNTATGNNNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT410.12363175828483551No Hit
CAGGAAANAGCTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA410.12363175828483551No Hit
NNGGANNNNGNTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG410.12363175828483551No Hit
NNGGANNNNGNTATGNNNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT400.12061634954618099No Hit
NNGGANNNNGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA400.12061634954618099No Hit
CAGGAAANAGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC400.12061634954618099No Hit
NNGGANNNNGNTATGNNNCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG400.12061634954618099No Hit
NAGGAAANAGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC400.12061634954618099No Hit
NNGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA390.11760094080752645No Hit
NAGGAAANAGCTATGANNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC390.11760094080752645No Hit
NNGGANNNNGNTATGNNNGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC390.11760094080752645No Hit
NNGGANNNNGNTATGNNNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA380.11458553206887194No Hit
NNGGANNNNGNTATGANNACACCCGGAGCAGTGCAAATCAACTCCCCCAG380.11458553206887194No Hit
NAGGAAANAGCTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA370.11157012333021742No Hit
NAGGAAANAGNTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA370.11157012333021742No Hit
NAGGAAANAGCTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT370.11157012333021742No Hit
NNGGAAANNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT370.11157012333021742No Hit
NNGGANNNNGNTATGNNNTGTTTTGCCCCTGAAATGAATATCTAAACCAG370.11157012333021742No Hit
NNGGANNNNGNTATGNNNGCTCCTGTGCTGTCAAGGGACTCCTGTAGTGG370.11157012333021742No Hit
NNGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGACACTGCCCACC360.10855471459156288No Hit
NNGGANNNNGNTATGNNNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT360.10855471459156288No Hit
NAGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT360.10855471459156288No Hit
NAGGAANNNGNTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT360.10855471459156288No Hit
CAGGAAANAGCTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT360.10855471459156288No Hit
NAGGANNNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC360.10855471459156288No Hit
NAGGANNNNGNTATGNNNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC360.10855471459156288No Hit
NNGGANNNNGNTATGANNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT360.10855471459156288No Hit
NAGGAAANAGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT360.10855471459156288No Hit
NAGGAAANAGCTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT350.10553930585290837No Hit
NAGGAANNNGNTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA350.10553930585290837No Hit
NNGGANNNNGNTATGNNNGAGGCGGAAGTGGTGCTCATCCACGTACTGTG350.10553930585290837No Hit
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT350.10553930585290837No Hit
NAGGANNNNGNTATGNNNCAGGGCTCCAAGAATGTTCCACACATCTTTCT350.10553930585290837No Hit
NNGGAAANNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC350.10553930585290837No Hit
NAGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC350.10553930585290837No Hit
NAGGANNNNGNTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT340.10252389711425383No Hit
NNGGANNNNGNTATGANNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG340.10252389711425383No Hit
NNGGANNNNGNTATGNNNCATGTGCCCCAGTAGAGAGCAATTAAATTCCT340.10252389711425383No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)