FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50322
Sequences flagged as poor quality0
Sequence length300
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA9091.8063669965422677No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA8591.707006875720361No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC6681.3274512141806765No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT6481.2877071658519137No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC6131.218155081276579No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT5721.1366797822026151No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC5381.0691149000437183No Hit
CAGGAAACAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT5311.0552044831286516No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA5030.9995628154683837No Hit
CAGGAAACAGCTATGACCCATCAGGCCCCACCTCCAACCCCGTTAGCTGC4480.890266682564286No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGCACTCCTTC4360.8664202535670283No Hit
CAGGAAACAGCTATGACCCCGTAGCCACGCCCCTATTGTCTGACCACGCT4130.8207145979889512No Hit
CAGGAAANAGCTATGACCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA4110.8167401931560748No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC3650.7253288819999205No Hit
CAGGAAACAGCTATGACCCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT3590.7134056675012916No Hit
CAGGAAANAGCTATGACCCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA3590.7134056675012916No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCCCCTCTTCTAGG3530.7014824530026629No Hit
CAGGAAACAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG3360.6677000119232145No Hit
CAGGAAACAGCTATGACCAGAGGCACAGGTGGAGAGGGACGAGACCCTGA3230.6418663805095187No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA3210.6378919756766424No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACCAGGGCAGCGGT3070.6100711418465085No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT3020.6001351297643178No Hit
CAGGAAANAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC3010.5981479273478797No Hit
CAGGAAACAGCTATGACCTCCCTTAAACTTGGGATTGCTCCATTGCACTC3010.5981479273478797No Hit
CAGGAAACAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC2930.5822503080163746No Hit
CAGGAAACAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG2860.5683398911013076No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCTCCCCGCCAAGA2800.5564166766026788No Hit
CAGGAAACAGCTATGACCTGGGCCTGGACACTTTGACGGCGGGGCACGTC2770.5504550693533643No Hit
CAGGAAANAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC2750.546480664520488No Hit
CAGGAAANAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT2610.5186598306903542No Hit
CAGGAAANAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT2410.4789157823615914No Hit
CAGGAAANAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT2380.47295417511227694No Hit
CAGGAAANAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC2330.46301816303008625No Hit
NAGGAAANAGCTATGACCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA2320.4610309606136481No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCACCCCACCCTGCT2210.4391717340328286No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC2200.4371845316163904No Hit
CAGGAAACAGCTATGACCATCCACTGTTGGCCAACACACCTGGCCTTCAA2190.4351973291999523No Hit
CAGGAAACAGCTATGACCGCTCCTGTGCTGTCAAGGGACTCCTGTAGTGG2180.43321012678351417No Hit
CAGGAAACAGCTATGACCAAACCCTCGACCCTGAGGCTGGAAGCGCCCCA2150.42724851953419973No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC2110.41929970986844717No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT2070.41135090020269466No Hit
CAGGAAACAGCTATGACCGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC1970.3914788760383133No Hit
CAGGAAANAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA1930.3835300663725607No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACCCGTCCTCGGAC1840.36564524462461745No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGCTGCCTGGCTTG1830.3636580422081793No Hit
CAGGAAACAGCTATGACCTCAAGCAAGTTCAGGATGGTATAGAAATAGAG1810.35968363737530307No Hit
CAGGAAANAGCTATGACCCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT1790.35570923254242676No Hit
CAGGAAACAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT1780.35372203012598863No Hit
CAGGAAANAGCTATGACCCCGTAGCCACGCCCCTATTGTCTGACCACGCT1760.3497476252931124No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCTGGGTGCTGGTG1720.3417988156273598No Hit
CAGGAAANAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1700.3378244107944835No Hit
CAGGAAANAGCTATGACCTGGAGTTTGAAACAGCACCTTGGCACTCCTTC1650.3278883987122928No Hit
CAGGAAANAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG1650.3278883987122928No Hit
CAGGAAACAGCTATGACCGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA1610.31993958904654024No Hit
NAGGAAANAGCTATGACCCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA1600.31795238663010217No Hit
CAGGAAACAGCTATGACCACACATACTCCCTTGCTCCAGGGCTGCAGAAC1600.31795238663010217No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGGCACGGGAACA1540.3060291721314733No Hit
CAGGAAANAGCTATGACCAGTGGGAGGAGCTCTCGGTCCCCTCTTCTAGG1480.2941059576328445No Hit
CAGGAAANAGCTATGACCCATCAGGCCCCACCTCCAACCCCGTTAGCTGC1450.2881443503835301No Hit
CAGGAAACAGCTATGACCTGGGGATGAGGACAGCAGGGCAGTCACCATCT1430.2841699455506538No Hit
CAGGAAANAGCTATGACCTAGAGGACACCCAAGGCCACCAGGGCAGCGGT1430.2841699455506538No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACCCTTTGTCCA1390.2762211358849012No Hit
CAGGAAACAGCTATGACCCATCAGGCCCCACCTCCAACCAGGAAACAGCT1390.2762211358849012No Hit
CAGGAAACAGCTATGACCTTTTTGTGCCCTTCTGCCCTGCCAGGAGCAGG1360.27025952863558683No Hit
CAGGAAACAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA1350.2682723262191487No Hit
CAGGAAANAGCTATGACCACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA1330.2642979213862724No Hit
NAGGAAANAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC1320.26231071896983427No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTAGGAGGGCTGCG1320.26231071896983427No Hit
CAGGAAANAGCTATGACCAGAGGCACAGGTGGAGAGGGACGAGACCCTGA1300.258336314136958No Hit
CAGGAAACAGCTATGACCCCTGGACGCAATCACCCAGGCTGCAGTGCGGT1280.2543619093040817No Hit
CAGGAAANAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG1270.2523747068876436No Hit
CAGGAAANAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT1260.25038750447120545No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAAGGCCGCAGCTCC1240.24641309963832916No Hit
CAGGAAANAGCTATGACCTGGGCCTGGACACTTTGACGGCGGGGCACGTC1240.24641309963832916No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGATTCTTTT1230.24442589722189104No Hit
CAGGAAANAGCTATGACCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC1230.24442589722189104No Hit
CAGGAAACAGCTATGACCTTTTTGTGCCCTTCTGCCCATGCTGGGTGGGA1220.24243869480545288No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGATACTACTA1220.24243869480545288No Hit
NAGGAAANAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC1180.23448988513970034No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCGTGTGCGCTGGA1180.23448988513970034No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACGGAGATCCAGCC1160.23051548030682406No Hit
CAGGAAANAGCTATGACCTCCCTTAAACTTGGGATTGCTCCATTGCACTC1140.22654107547394778No Hit
CAGGAAANAGCTATGACCGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT1140.22654107547394778No Hit
NAGGAAANAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1130.22455387305750965No Hit
NAGGAAANAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT1130.22455387305750965No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGCTGAATAGTTGT1120.2225666706410715No Hit
NAGGAAANAGCTATGACCCCGTAGCCACGCCCCTATTGTCTGACCACGCT1120.2225666706410715No Hit
CAGGAAANAGCTATGACCACCTCTCTGGGCCTGTCTCCTCCCCGCCAAGA1120.2225666706410715No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGGTCCTCCTC1110.22057946822463337No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCTGGGTGTCTGC1080.21461786097531896No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGACACACCACT1080.21461786097531896No Hit
CAGGAAANAGCTATGACCGCTCCTGTGCTGTCAAGGGACTCCTGTAGTGG1080.21461786097531896No Hit
NAGGAAANAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC1060.21064345614244268No Hit
CAGGAAANAGCTATGACCAAGGACAAGGTGAGGCAGCACCCCACCCTGCT1050.20865625372600452No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCCGTGTGCGCTGG1050.20865625372600452No Hit
CAGGAAANAGCTATGACCGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC1050.20865625372600452No Hit
NAGGAAANAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA1040.2066690513095664No Hit
CAGGAAACAGCTATGACCTGGGGATGAGGACAGCAGGGCCCCGGGAGCTG1020.2026946464766901No Hit
CAGGAAANAGCTATGACCATCCACTGTTGGCCAACACACCTGGCCTTCAA1000.19872024164381383No Hit
CAGGAAACAGCTATGACCGGGTCCCACCCATGCTGAAAGTCCACCTTCCA980.19474583681093757No Hit
CAGGAAANAGCTATGACCTAGAGGACACCCAAGGCCACCCGTCCTCGGAC970.19275863439449942No Hit
CAGGAAANAGCTATGACCTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC960.1907714319780613No Hit
NAGGAAANAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC940.186797027145185No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT930.18480982472874688No Hit
CAGGAAACAGCTATGACCGTGCTCCTTGGGGACGTAGTCGCCTGGGGACT910.1808354198958706No Hit
NAGGAAANAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT890.17686101506299431No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTCACACCTGTAA880.1748738126465562No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCAAGCCTGACTG880.1748738126465562No Hit
CAGGAAANAGCTATGACCAAACCCTCGACCCTGAGGCTGGAAGCGCCCCA870.17288661023011803No Hit
CAGGAAACAGCTATGACCTTTTTGTGCCCTTCTGCCCAGCCAGGAGCAGG860.1708994078136799No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCAAGTGCTGATGTA850.16891220539724175No Hit
CAGGAAANAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC840.16692500298080362No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTGATCCCCCAAAA840.16692500298080362No Hit
CAGGAAANAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA840.16692500298080362No Hit
CAGGAAACAGCTATGACCTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC840.16692500298080362No Hit
CAGGAAANAGCTATGACCAGGACCACCCCTCCATCCTGCTGCCTGGCTTG830.16493780056436547No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGTCTGGGTCCT820.16295059814792737No Hit
CAGGAAANAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT800.15897619331505108No Hit
CAGGAAANAGCTATGACCGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA790.15698899089861293No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTCCCCAACCATAG790.15698899089861293No Hit
CAGGAAANAGCTATGACCACAGGAGACAGGATTGGGGCTGGGTGCTGGTG780.15500178848217477No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGTAGTCTC780.15500178848217477No Hit
NAGGAAANAGCTATGACCAGTGGGAGGAGCTCTCGGTCCCCTCTTCTAGG780.15500178848217477No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGTCTGCAGCCTGG760.15102738364929852No Hit
CAGGAAACAGCTATGACCTCTACGCCCCTCAGCTGTGTCAGGAAACAGCT730.1450657763999841No Hit
CAGGAAANAGCTATGACCTCAAGCAAGTTCAGGATGGTATAGAAATAGAG730.1450657763999841No Hit
CAGGAAANAGCTATGACCCGTTAGCTGCCTCTCCTGCCACCCTTTGTCCA720.14307857398354595No Hit
NAGGAAANAGCTATGACCCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT720.14307857398354595No Hit
NAGGAAANAGCTATGACCCATCAGGCCCCACCTCCAACCCCGTTAGCTGC710.14109137156710783No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGTGACACA690.13711696673423154No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTCCCTTTGGGCT690.13711696673423154No Hit
NAGGAAANAGCTATGACCTGGAGTTTGAAACAGCACCTTGGCACTCCTTC670.13314256190135526No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTCACGCCTGTAA660.13115535948491713No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCAGGCAAACTTGG660.13115535948491713No Hit
CAGGAAANAGCTATGACCCATCAGGCCCCACCTCCAACCAGGAAACAGCT650.129168157068479No Hit
CAGGAAANAGCTATGACCGGTAGCAACGTCCCACCCAGGGCACGGGAACA650.129168157068479No Hit
CAGGAAACAGCTATGACCGGGGAATGGGAAGGGAGATGAAAGAGTAGGTG650.129168157068479No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGCCTGGCTTGTC650.129168157068479No Hit
CAGGAAANAGCTATGACCGGTAGCAACGTCCCACCCAGCTGAATAGTTGT650.129168157068479No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCAGCACAGGGAGT640.12718095465204085No Hit
CAGGAAANAGCTATGACCTGGGGATGAGGACAGCAGGGCAGTCACCATCT640.12718095465204085No Hit
NAGGAAANAGCTATGACCTCCCTTAAACTTGGGATTGCTCCATTGCACTC620.12320654981916458No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTTCCCTTTGGGC620.12320654981916458No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC610.12121934740272644No Hit
CAGGAAANAGCTATGACCTTTTTGTGCCCTTCTGCCCTGCCAGGAGCAGG610.12121934740272644No Hit
CAGGAAACAGCTATGACCTGGGGATGAGGACAGCAGGGGGAGGGAGCTGC600.1192321449862883No Hit
NAGGAAANAGCTATGACCACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA600.1192321449862883No Hit
NAGGAAANAGCTATGACCTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC600.1192321449862883No Hit
CAGGAAANAGCTATGACCACACATACTCCCTTGCTCCAGGGCTGCAGAAC600.1192321449862883No Hit
CAGNAAANAGCTATGACCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA600.1192321449862883No Hit
CAGGAAACAGCTATGACCTCCAGCCAGCCAGTCCTTGTAACTCAGCATGA580.11525774015341203No Hit
NAGGAAANAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG570.11327053773697389No Hit
NAGGAAANAGCTATGACCTAGAGGACACCCAAGGCCACCAGGGCAGCGGT570.11327053773697389No Hit
CAGGAAANAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGATTCTTTT570.11327053773697389No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGGGGGCCTGGCT560.11128333532053575No Hit
CAGGAAACAGCTATGACCCCTGGACGCAATCACCCAGGCCAGCTAAGTGA560.11128333532053575No Hit
NAGGAAANAGCTATGACCATCCACTGTTGGCCAACACACCTGGCCTTCAA560.11128333532053575No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGGAGTCAC550.1092961329040976No Hit
NAGGAAANAGCTATGACCACCTCTCTGGGCCTGTCTCCTCCCCGCCAAGA540.10730893048765948No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCTATTTTCCAAAG540.10730893048765948No Hit
NAGGAAANAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG540.10730893048765948No Hit
CAGGAAANAGCTATGACCGCCTCTCAGGGCCTCACAGTAGGAGGGCTGCG540.10730893048765948No Hit
NAGGAAANAGCTATGACCGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT540.10730893048765948No Hit
CAGGAAANAGCTATGACCCCTGGACGCAATCACCCAGGCCAGCTAAGTGA530.10532172807122134No Hit
NAGGAAANAGCTATGACCTGGGCCTGGACACTTTGACGGCGGGGCACGTC530.10532172807122134No Hit
CAGGAAACAGCTATGACCTGGGGATGAGGACAGCAGGGGGCTGAAGAAGA530.10532172807122134No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCTCCGTGTGCGCT520.1033345256547832No Hit
CAGGAAACAGCTATGACCAGAGGCACAGGTGGAGAGGGGGTGATGGCCAT520.1033345256547832No Hit
NAGGAAANAGNTATGANCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA510.10134732323834506No Hit
CAGGAAACAGCTATGACCGGCGGGCCCTGATATAGAGCCACTGGCCGTCG510.10134732323834506No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)