FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115318
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC6190.5367765656705805No Hit
NNGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC5670.4916838654850067No Hit
NNGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT5290.45873150765708737No Hit
NNGGANNNNGNTATGNNNTGTTTTGCCCCTGAAATGAATATTCATCGTCT5200.4509270018557381No Hit
NNGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT5130.44485683067691084No Hit
NNGGANNNNGNTATGNNNTGACTGAAAGGCTTTTCCACGTGCACTGCTGG4920.42664631714042905No Hit
NNGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC4590.39802979586881493No Hit
NNGGANNNNGNTATGNNNTGACAGCACAAGGAACACTGACCCGAGGCAGG4570.3962954612462929No Hit
NNGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA4540.3936939593125097No Hit
NNGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC4230.3668117726634177No Hit
NNGGANNNNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT4210.3650774380408956No Hit
NNGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT4070.3529370956832411No Hit
NNGGANNNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT3980.3451325898818918No Hit
NNGGANNNNGNTATGNNNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA3970.34426542257063075No Hit
NNGGANNNNGNTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA3800.3295235782791932No Hit
NNGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG3770.3269220763454101No Hit
NNGGANNNNGNTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT3750.325187741722888No Hit
NNGGANNNNGNTATGANNTGACTGAAAGGCTTTTCCACGTGCACTGCTGG3530.30611006087514525No Hit
NNGGANNNNGNTATGNNNACAGTGTCCCCATCAGGCTCCGGGACAGCATC3340.28963388196118556No Hit
NNGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC3210.27836070691479214No Hit
NNGGANNNNGNTATGNNNTGGGGATGAGGACAGCAGGGCAGTCACCATCT3210.27836070691479214No Hit
NNGGANNNNGNTATGNNNAGGCACCAACAGTACAAAGCAGATACAAAGCA3200.27749353960353107No Hit
NNGGANNNNGNTATGANNTGACAGCACAAGGAACACTGACCCGAGGCAGG3100.26882186649092077No Hit
NNGGANNNNGNTATGANNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT2960.2566815241332663No Hit
NAGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC2880.24974418564317802No Hit
NAGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2770.24020534521930664No Hit
NNGGANNNNGNTATGNNNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT2650.22979933748417417No Hit
NNGGANNNNGNTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA2600.22546350092786904No Hit
NNGGANNNNGNTATGNNNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT2590.224596333616608No Hit
NNGGANNNNGNTATGNNNAGCTGGACGGGGTCCTTCTTCAGGAAACAGCT2580.22372916630534695No Hit
NNGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG2570.2228619989940859No Hit
NNGGANNNNGNTATGNNNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC2570.2228619989940859No Hit
NNGGANNNNGNTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA2570.2228619989940859No Hit
NNGGANNNNGNTATGNNNGGTAGCAACGTCCCACCCAGGGCACGGGAACA2560.22199483168282488No Hit
NAGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT2550.22112766437156384No Hit
NNGGANNNNGNTATGNNNACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA2550.22112766437156384No Hit
NNGGANNNNGNTATGNNNTGCTGGGAGAGGGGGATGAGTAGACAGGGCCA2530.21939332974904177No Hit
NAGGANNNNGNTATGNNNTGTTTTGCCCCTGAAATGAATATTCATCGTCT2510.21765899512651973No Hit
NAGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC2510.21765899512651973No Hit
NNGGANNNNGNTATGNNNCTTGAGCTCCTCGGACACGGCAGGAAACAGCT2500.2167918278152587No Hit
NNGGANNNNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2390.20725298739138728No Hit
NNGGANNNNGNTATGNNNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC2330.2020499835238211No Hit
NNGGANNNNGNTATGNNNGCTGCTGACCCAACCCTACCTCTGCAGCTAGC2250.1951126450337328No Hit
NNGGANNNNGNTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT2220.1925111430999497No Hit
NNGGANNNNGNTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC2200.19077680847742765No Hit
NAGGANNNNGNTATGNNNTGACTGAAAGGCTTTTCCACGTGCACTGCTGG2190.1899096411661666No Hit
NNGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA2160.1873081392323835No Hit
NAGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA2150.18644097192112247No Hit
NNGGANNNNGNTATGANNAGCTGGACGGGGTCCTTCTTCAGGAAACAGCT2140.18557380460986142No Hit
NNGGANNNNGNTATGANNTGCTGGGAGAGGGGGATGAGTAGACAGGGCCA2060.17863646611977316No Hit
NNGGANNNNGNTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC2020.17516779687472903No Hit
NNGGANNNNGNTATGNNNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1970.17083196031842382No Hit
NNGGANNNNGNTATGNNNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA1950.16909762569590178No Hit
NNGGANNNNGNTATGANNGGTAGCAACGTCCCACCCAGGGCACGGGAACA1940.16823045838464074No Hit
NAGGANNNNGNTATGNNNTGACAGCACAAGGAACACTGACCCGAGGCAGG1920.16649612376211867No Hit
NAGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC1910.16562895645085762No Hit
NNGGANNNNGNTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT1880.16302745451707454No Hit
NNGGANNNNGNTATGNNNTGCTGCCGAGAGAGGACAGTCCCCAACCATAG1870.1621602872058135No Hit
NNGGANNNNGNTATGNNNCAGGGCTCCAAGAATGTTCCACACATCTTTCT1870.1621602872058135No Hit
NNGGANNNNGNTATGANNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC1850.16042595258329143No Hit
NNGGANNNNGNTATGNNNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC1850.16042595258329143No Hit
NNGGANNNNGNTATGNNNTCCCTTAAACTTGGGATTGCTCCATTGCACTC1810.1569572833382473No Hit
NNGGANNNNGNTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT1810.1569572833382473No Hit
NAGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT1790.1552229487157252No Hit
NNGGANNNNGNTATGANNACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA1770.15348861409320316No Hit
NNGGANNNNGNTATGNNNGCCTCTCAGGGCCTCACAGTAGGAGGGCTGCG1750.15175427947068107No Hit
NNGGANNNNGNTATGANNCTTGAGCTCCTCGGACACGGCAGGAAACAGCT1750.15175427947068107No Hit
NNGGANNNNGNTATGNNNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA1750.15175427947068107No Hit
NAGGANNNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1730.150019944848159No Hit
NNGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACCCTTTGTCCA1690.14655127560311487No Hit
NAGGANNNNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1680.14568410829185383No Hit
NNGGANNNNGNTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA1620.14048110442428763No Hit
NNGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA1620.14048110442428763No Hit
NNGGANNNNGNTATGNNNGGAAGGCAGGTGGTGGGAACGGCCCCTCTGCC1620.14048110442428763No Hit
NAGGANNNNGNTATGNNNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1580.1370124351792435No Hit
NNGGANNNNGNTATGNNNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT1540.13354376593419934No Hit
NNGGANNNNGNTATGANNGCTGCTGACCCAACCCTACCTCTGCAGCTAGC1520.13180943131167727No Hit
NAGGANNNNGNTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA1520.13180943131167727No Hit
NNGGANNNNGNTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC1510.13094226400041625No Hit
NAGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG1500.1300750966891552No Hit
NAGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC1490.12920792937789416No Hit
NNGGANNNNGNTATGNNNGGTAGCAACGTCCCACCCAGGCTCCTCCTCGC1460.12660642744411105No Hit
NAGGANNNNGNTATGNNNACAGTGTCCCCATCAGGCTCCGGGACAGCATC1460.12660642744411105No Hit
NNGGANNNNGNTATGNNNGGGAGGTGGAGGTTGCAGTGAGCCGAGACGGC1450.12573926013285003No Hit
NNGGANNNNGNTATGANNTCCCTTAAACTTGGGATTGCTCCATTGCACTC1440.12487209282158901No Hit
NNGGANNNNGNTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1440.12487209282158901No Hit
NAGGANNNNGNTATGNNNAGGCACCAACAGTACAAAGCAGATACAAAGCA1430.12400492551032796No Hit
NNGGANNNNGNTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA1430.12400492551032796No Hit
NAGGANNNNGNTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1420.12313775819906693No Hit
NNGGANNNNGNTATGNNNAAGGACAAGGTGAGGCAGCACTGAACTGACAT1420.12313775819906693No Hit
NNGGANNNNGNTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT1410.1222705908878059No Hit
NNGGANNNNGNTATGANNGCCTCTCAGGGCCTCACAGTAGGAGGGCTGCG1380.1196690889540228No Hit
NNGGANNNNGNTATGNNNTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC1350.11706758702023969No Hit
NNGGANNNNGNTATGANNTGCTGCCGAGAGAGGACAGTCCCCAACCATAG1340.11620041970897864No Hit
NAGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1330.1153332523977176No Hit
NAGGANNNNGNTATGANNTGACTGAAAGGCTTTTCCACGTGCACTGCTGG1330.1153332523977176No Hit
NNGGANNNNGNTATGANNGGGAGGTGGAGGTTGCAGTGAGCCGAGACGGC1330.1153332523977176No Hit
NNGGANNNNGNTATGANNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA1280.11099741584141244No Hit
NNGGANNNNGNTATGNNNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG1280.11099741584141244No Hit
NAGGANNNNGNTATGNNNTGGGGATGAGGACAGCAGGGCAGTCACCATCT1250.10839591390762934No Hit
NAGGANNNNGNTATGANNTGACAGCACAAGGAACACTGACCCGAGGCAGG1250.10839591390762934No Hit
NNGGANNNNGNTATGNNNCTGGAATTTCAACAGAATGGCATTTATATGAT1250.10839591390762934No Hit
NAGGANNNNGNTATGNNNAGCTGGACGGGGTCCTTCTTCAGGAAACAGCT1240.10752874659636831No Hit
NNGGANNNNGNTATGNNNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC1230.10666157928510726No Hit
NNGGANNNNGNTATGANNGGAAGGCAGGTGGTGGGAACGGCCCCTCTGCC1230.10666157928510726No Hit
NAGGANNNNGNTATGNNNGCTGCTGACCCAACCCTACCTCTGCAGCTAGC1210.1049272446625852No Hit
NNGGANNNNGNTATGNNNTGCTTCAGTCGCCTGCACAAGGGGACATGGCC1200.10406007735132415No Hit
NAGGANNNNGNTATGNNNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC1200.10406007735132415No Hit
NAGGANNNNGNTATGANNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT1190.10319291004006315No Hit
NAGGANNNNGCTATGANNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC1160.10059140810628002No Hit
NNGGANNNNGNTATGNNNCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG1160.10059140810628002No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)