FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106148
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT22982.164901835173531No Hit
CAGGAAACAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT22412.1112032256848927No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT22042.076346233560689No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA21602.0348946753589328No Hit
CAGGAAACAGCTATGACCTGACCATCTCTCCTAGACGCCAGTGCAGCTGC21312.0075743301805025No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT21121.9896747936842898No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC20601.9406865885367601No Hit
CAGGAAACAGCTATGACCCGTAAAGGGCAAGGTGGGGTAATCTTGTCCAT20101.8935825451256736No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC19711.8568413912650263No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA19101.7993744583035007No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG18981.78806948788484No Hit
CAGGAAACAGCTATGACCCAGGGCTCCAAGAATGTTCCACACATCTTTCT18741.7654595470475185No Hit
CAGGAAACAGCTATGACCACCCCACCTGCTCAAAAGACACACTCCTAAAA18061.7013980480084412No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACCAGGGCAGCGGT17751.6721935410935675No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC17681.6655989750160152No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC17261.626031578550703No Hit
CAGGAAACAGCTATGACCCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT16491.5534913516976299No Hit
CAGGAAACAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCATA16261.53182349172853No Hit
CAGGAAACAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA15611.4705882352941175No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA15431.4536307796661265No Hit
CAGGAAACAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG15301.4413837283792441No Hit
CAGGAAACAGCTATGACCGCTCCTGTGCTGTCAAGGGACTCCTGTAGTGG13071.2312996947657986No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC12991.223763047820025No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT12771.2030372687191468No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT12181.1474544974940648No Hit
CAGGAAACAGCTATGACCTGGCCCCAGTGTTGAGAGGTTCTTTTATTCCG12181.1474544974940648No Hit
CAGGAAACAGCTATGACCGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC11391.0730301089045484No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT11051.0409993593850098No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGCGGCCTTGCTGA10921.0287523080981271No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC10791.0165052568112447No Hit
CAGGAAACAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC10260.966574970795493No Hit
CAGGAAACAGCTATGACCAGATCCTGTGAAGTGGGAGGGTTTTCAAAGTC10260.966574970795493No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT10240.9646908090590496No Hit
CAGGAAACAGCTATGACCAGGACTTGCCCACTCTCCCAGTTACTTGGAAT9990.9411387873535064No Hit
CAGGAAACAGCTATGACCGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG9980.9401967064852847No Hit
CAGGAAACAGCTATGACCTGCTGGGAGAGGGGGATGAGCGGCGCCGCCAT9910.9336021404077325No Hit
CAGGAAACAGCTATGACCACAAACGCAAATGGCTTTTAGCATTTCACAGG9670.9109921995704111No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCTGTCCAGCCCC9540.8987451482835286No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT8170.7696800693371519No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT8050.7583750989184911No Hit
CAGGAAACAGCTATGACCGCCCCTACTCCAAAAGGCGGATGAATGGGGAA7970.7508384519727174No Hit
CAGGAAACAGCTATGACCGGAGAAGCACAGCTTCGGCTCTGGCCCTGGGG7810.7357651580811697No Hit
CAGGAAACAGCTATGACCGGGTCCCACCCATGCTGAAAGTCCACCTTCCA7800.734823077212948No Hit
CAGGAAACAGCTATGACCGTGCTCCTTGGGGACGTAGTCGCCTGGGGACT7370.6943135998794137No Hit
CAGGAAACAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC7190.6773561442514225No Hit
CAGGAAACAGCTATGACCCTGGGTGCCGGGTAGAGTGTATACAGGGCTCA7160.6745299016467574No Hit
CAGGAAACAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGTC6990.658514526886988No Hit
CAGGAAACAGCTATGACCCACCGCTGTCCCTTCCCAAGGGGCTGAAGGTG5650.5322756905452763No Hit
CAGGAAACAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA5530.5209707201266156No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAATGAAACTTGGTT5520.5200286392583939No Hit
CAGGAAACAGCTATGACCTCCAGTAAAACTGCCTCTGTTTCAACTCCCTT4920.4635037871650902No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGTGACACA4870.4587933828239816No Hit
CAGGAAACAGCTATGACCAAAACCAAGTCACCTGTTGATCTGTCAGGAAA4810.45314089761465126No Hit
CAGGAAACAGCTATGACCTCCCTGCTTGATAATGACCCCTCTGAATTCTT4630.43618344198666015No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCTATTTTCCAAAG3790.35704864905603495No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCATGTGTTCAGT3660.3448015977691525No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAATTCCATTCT3440.3240758186682745No Hit
CAGGAAACAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGTGGAGACGGC3350.31559709085427895No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAATTTCATTCT3250.30617628217206166No Hit
CAGGAAACAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGCCAAGACGGC3170.29863963522628784No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTATTCCCAACCA3160.2976975543580661No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA3160.2976975543580661No Hit
CAGGAAACAGCTATGACCTCCAGCCAGCCAGTCCTTGTAACTCAGCATGA3130.29487131175340087No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTACTCCCAACCA3120.2939292308851792No Hit
CAGGAAACAGCTATGACCTTGCCAGTGCTGCCTTCAGCCATTTGTGAAAT2960.27885593699363154No Hit
CAGGAAACAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGCCCATACGGC2930.27602969438896635No Hit
CAGGAAACAGCTATGACCGATGCTCCTTCCTTGGCCCCCACTCCTCCCCC2690.25341975355164487No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCGGGTCTTACA2610.24588310660587104No Hit
CAGGAAACAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGCCGAGACGGC2510.23646229792365375No Hit
CAGGAAACAGCTATGACCGATGGCAGAATGGAAACATGGCAAAAGAAGAG2390.225157327504993No Hit
CAGGAAACAGCTATGACCGATGGCAGAATGGAAACATGGCAAAAAAGAGG2380.2242152466367713No Hit
CAGGAAACAGCTATGACCACCCAGTTAGAGCCATGGTTCCACTAGACTCT2050.19312657798545427No Hit
CAGGAAACAGCTATGACCGCCATGCTTGAATCTTCGCAGTTTAGCTGTAA1650.15544334325658515No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGAAGCTCCAGCA1520.14319629196970268No Hit
CAGGAAACAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGCCCAGACGGC1350.1271809172099333No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA1310.12341259373704638No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTAGGAGGGCTGCG1270.11964427026415947No Hit
CAGGAAACAGCTATGACCCAGTTGCTTGGGGTTTGTGTCTTATGATGTTT1240.1168180276594943No Hit
CAGGAAACAGCTATGACCACCCCTTGCATTGGTAGGGCTTCTCTCCTGTG1230.11587594679127255No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)