FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91707
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT4400.47978889288712967No Hit
NNGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3920.4274482863903519No Hit
NNGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT3640.39691626593389817No Hit
NNGGANNNNGNTATGNNNTGTTTTGCCCCTGAAATGAATATTCATCGTCT3490.3805598264036551No Hit
NNGGANNNNGNTATGNNNGTCTCAGGGATCACGTGGCGCAGACCTCGCTC3140.342394800833088No Hit
NNGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC3090.33694265432300696No Hit
NNGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC3060.3336713664169584No Hit
NNGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2990.3260383613028449No Hit
NNGGANNNNGNTATGNNNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA2920.3184053561887315No Hit
NNGGANNNNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2830.30859149247058565No Hit
NNGGANNNNGNTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT2830.30859149247058565No Hit
NNGGANNNNGNTATGNNNAGGCACCAACAGTACAAAGCAGATACAAAGCA2650.288963765034294No Hit
NNGGANNNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT2610.2846020478262292No Hit
NNGGANNNNGNTATGNNNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC2590.2824211892221968No Hit
NNGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT2570.28024033061816433No Hit
NNGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC2440.2660647496919537No Hit
NNGGANNNNGNTATGNNNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT2430.2649743203899375No Hit
NNGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2330.25407002736977546No Hit
NNGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA2300.2507987394637269No Hit
NNGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC2250.24534659295364583No Hit
NNGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG2240.24425616365162964No Hit
NNGGANNNNGNTATGANNGTCTCAGGGATCACGTGGCGCAGACCTCGCTC2230.24316573434961347No Hit
NNGGANNNNGNTATGNNNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT2180.23771358783953242No Hit
NNGGANNNNGNTATGNNNACAGTGTCCCCATCAGGCTCCGGGACAGCATC2180.23771358783953242No Hit
NNGGANNNNGNTATGNNNGGGACCAGTCCAATAACCCCTGAGGGGTATAT2150.2344422999334838No Hit
NAGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2080.2268092948193704No Hit
NNGGANNNNGNTATGNNNTCCTGCCCCACCCTGATACAGGTCACTGTGGA2060.22462843621533796No Hit
NNGGANNNNGNTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA2050.22353800691332176No Hit
NAGGANNNNGNTATGNNNTGTTTTGCCCCTGAAATGAATATTCATCGTCT2000.21808586040324074No Hit
NNGGANNNNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2000.21808586040324074No Hit
NNGGANNNNGNTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1950.21263371389315974No Hit
NNGGANNNNGNTATGNNNATCCACTGTTGGCCAACACACCTGGCCTTCAA1930.21045285528912733No Hit
NNGGANNNNGNTATGANNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT1900.2071815673830787No Hit
NAGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1900.2071815673830787No Hit
NNGGANNNNGNTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC1860.2028198501750139No Hit
NNGGANNNNGNTATGANNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT1840.20063899157098147No Hit
NNGGANNNNGNTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA1840.20063899157098147No Hit
NNGGANNNNGNTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC1820.19845813296694909No Hit
NNGGANNNNGNTATGNNNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT1810.1973677036649329No Hit
NNGGANNNNGNTATGNNNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1810.1973677036649329No Hit
NAGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1800.19627727436291667No Hit
NNGGANNNNGNTATGNNNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC1780.19409641575888426No Hit
NNGGANNNNGNTATGANNTCCTGCCCCACCCTGATACAGGTCACTGTGGA1650.17992083483267363No Hit
NNGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG1640.1788304055306574No Hit
NNGGANNNNGNTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT1620.17664954692662502No Hit
NNGGANNNNGNTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC1600.1744686883225926No Hit
NNGGANNNNGNTATGNNNAGAGGCACAGGTGGAGAGGGACGAGACCCTGA1580.17228782971856021No Hit
NNGGANNNNGNTATGNNNCAGGGCTCCAAGAATGTTCCACACATCTTTCT1560.17010697111452777No Hit
NNGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC1560.17010697111452777No Hit
NNGGANNNNGNTATGNNNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC1530.1668356832084792No Hit
NNGGANNNNGNTATGNNNCCTGGACGCAATCACCCAGGCTGCAGTGCGGT1530.1668356832084792No Hit
NAGGANNNNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1510.16465482460444678No Hit
NNGGANNNNGNTATGNNNACCTCTCTGGGCCTGTCTCCTCCCCGCCAAGA1500.16356439530243058No Hit
NNGGANNNNGNTATGANNATCCACTGTTGGCCAACACACCTGGCCTTCAA1450.15811224879234956No Hit
NAGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC1450.15811224879234956No Hit
NNGGANNNNGNTATGNNNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA1440.15702181949033334No Hit
NNGGANNNNGNTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT1390.15156967298025234No Hit
NNGGANNNNGNTATGNNNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT1380.15047924367823612No Hit
NAGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT1350.1472079557721875No Hit
NNGGANNNNGNTATGNNNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT1350.1472079557721875No Hit
NAGGANNNNGNTATGNNNGTCTCAGGGATCACGTGGCGCAGACCTCGCTC1350.1472079557721875No Hit
NAGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1340.14611752647017132No Hit
NAGGANNNNGNTATGNNNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1320.1439366678661389No Hit
NAGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC1310.1428462385641227No Hit
NNGGANNNNGNTATGNNNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG1290.1406653799600903No Hit
NNGGANNNNGNTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT1280.13957495065807407No Hit
NAGGANNNNGNTATGNNNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC1280.13957495065807407No Hit
NAGGANNNNGNTATGNNNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT1270.13848452135605788No Hit
NAGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA1270.13848452135605788No Hit
NNGGANNNNGNTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT1230.13412280414799307No Hit
NAGGANNNNGNTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1210.13194194554396066No Hit
NAGGANNNNGNTATGNNNTCCTGCCCCACCCTGATACAGGTCACTGTGGA1190.12976108693992824No Hit
NNGGANNNNGNTATGNNNGGTAGCAACGTCCCACCCAGGGCACGGGAACA1180.12867065763791205No Hit
NNGGANNNNGNTATGNNNGGGACCAGTCCAATAACCCCTGACAGGAAACA1160.12648979903387964No Hit
NNGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC1160.12648979903387964No Hit
NNGGANNNNGNTATGANNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA1150.12539936973186344No Hit
NAGGANNNNGNTATGNNNAGGCACCAACAGTACAAAGCAGATACAAAGCA1150.12539936973186344No Hit
NNGGANNNNGNTATGNNNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA1140.12430894042984722No Hit
NNGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACCCTTTGTCCA1130.12321851112783104No Hit
NNGGANNNNGNTATGANNAGAGGCACAGGTGGAGAGGGACGAGACCCTGA1110.12103765252379861No Hit
NNGGANNNNGNTATGNNNTCCCTTAAACTTGGGATTGCTCCATTGCACTC1110.12103765252379861No Hit
NAGGANNNNGNTATGNNNGGGACCAGTCCAATAACCCCTGAGGGGTATAT1090.11885679391976621No Hit
NAGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1080.11776636461775No Hit
NNGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCAGGAAACAGCT1080.11776636461775No Hit
NAGGANNNNGNTATGNNNACAGTGTCCCCATCAGGCTCCGGGACAGCATC1080.11776636461775No Hit
NNGGANNNNGNTATGNNNAGAGACCAGATGCATAAGCACAGCAGCATTCC1060.1155855060137176No Hit
NNGGANNNNGNTATGANNCCTGGACGCAATCACCCAGGCTGCAGTGCGGT1050.11449507671170141No Hit
NNGGANNNNGNTATGNNNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT1030.11231421810766898No Hit
NNGGANNNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC1020.11122378880565278No Hit
NNGGANNNNGNTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC1010.11013335950363658No Hit
NNGGANNNNGNTATGANNTCCCTTAAACTTGGGATTGCTCCATTGCACTC1010.11013335950363658No Hit
NAGGANNNNGNTATGNNNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1010.11013335950363658No Hit
NNGGANNNNGNTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1010.11013335950363658No Hit
NAGGANNNNGNTATGANNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT1000.10904293020162037No Hit
NNGGANNNNGNTATGNNNTCCTGGAACCTGCCTCCGAAGGCCCCATCCGG990.10795250089960418No Hit
NAGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC950.10359078369153935No Hit
NNGGANNNNGNTATGANNGGGACCAGTCCAATAACCCCTGACAGGAAACA950.10359078369153935No Hit
NNGGANNNNGNTATGNNNGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA950.10359078369153935No Hit
NAGGANNNNGNTATGNNNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC940.10250035438952317No Hit
NAGGANNNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT940.10250035438952317No Hit
NNGGANNNNGNTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT930.10140992508750694No Hit
NAGGANNNNGNTATGANNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT930.10140992508750694No Hit
NNGGANNNNGNTATGNNNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG920.10031949578549074No Hit
NAGGANNNNGNTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA920.10031949578549074No Hit
NNGGANNNNGNTATGNNNTGGCATCCAGGGCCTATTTGGTTCCTATTGAG920.10031949578549074No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)