FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128926
Sequences flagged as poor quality0
Sequence length300
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTAGCACGAAAAGTAGGTGGCCTTACTGGTAGCA33282.581325721731846No Hit
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG19401.504739152692242No Hit
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA17601.3651241797620342No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTGGTGGTCC17441.3527139599460156No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGGTTGGAGGTG16751.2991948869894359No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGTAAAACGAC16211.2573103951103735No Hit
TGTAAAACGACGGCCAGTACTAAACTGTAGTGAACAGAGACATGCCCAGG15471.1999131284612878No Hit
TGTAAAACGACGGCCAGTACCTCATGTCACGTGCGTCCACAGTGACCTGT14531.1270030870421792No Hit
TGTAAAACGACGGCCAGTAGGTCATCCTCCTGCCTCGGGTCAGTGTTCCT14111.0944262600251307No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCTCCTTCCCAT12890.9997983339279898No Hit
TGTAAAACGACGGCCAGTTGTAGCCCCCAGGAAGAGGGCGCGGAGGCAGC12530.9718753393419481No Hit
TGTAAAANGACGGCCAGTAGCACGAAAAGTAGGTGGCCTTACTGGTAGCA12030.9330934024168904No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG12020.9323177636783891No Hit
TGTAAAACGACGGCCAGTGGGGAGTGAGAGGCCATAGCTGTCTGGCATGG11840.9183562663853683No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG10830.8400167537967516No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGTGTAAAACGACG10820.8392411150582505No Hit
TGTAAAACGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC10720.8314847276732389No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG10450.8105424817337077No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG10220.7927027907481812No Hit
TGTAAAACGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT10170.7888245970556753No Hit
TGTAAAACGACGGCCAGTCCCAGCACAACTTCCCCCATGGCGTGGTGTCC9560.7415106340071049No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC9490.7360811628375967No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG9280.7197927493290724No Hit
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA9220.7151389168980655No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT9200.7135876394210632No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCAGCAGGATGGAG9020.6996261421280425No Hit
TGTAAAACGACGGCCAGTCAGCCAGTAGCAACAGCAGCTACTGATGTAAG8940.6934210322200332No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGTCAT8730.677132618711509No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC7760.6018956610768968No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCCAAGAGGTGGGG7630.5918123574763818No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCATGACAAGGCT7180.5569086142438298No Hit
TGTAAAANGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA6900.5351907295657974No Hit
TGTAAAANGACGGCCAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG6560.5088190124567581No Hit
TGTAAAACGACGGCCAGTGGCTCCCCAGCTGTCTCTCTTGTAAAACGACG6260.48554985030172343No Hit
TGTAAAANGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTGGTGGTCC5930.4599537719311853No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA5860.45452430076167727No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC5760.4467679133766657No Hit
TGTAAAACGACGGCCAGTCCATCCCACAGCCATCCGTGACTGTGACGACA5700.44211408094565874No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCACATGCCTCCC5670.4397871647301553No Hit
TGTAAAACGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT5580.4328064160836449No Hit
TGTAAAANGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGGTTGGAGGTG5520.428152583652638No Hit
TGTAAAANGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGTAAAACGAC5500.42660130617563563No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC5240.4064346989746056No Hit
TGTAAAANGACGGCCAGTACTAAACTGTAGTGAACAGAGACATGCCCAGG5110.3963513953740906No Hit
TGTAAAACGACGGCCAGTTGAGAAGCTGAGGCTTAAAGAGGGATCCCTTG5040.39092192420458244No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC5020.38937064672758015No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC4880.37851170438856396No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGTCTTGGCTCACT4780.37075531700355246No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCTCCTGCTCAGCCTT4660.36144765214153857No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGTCTCGGCTCACT4560.353691264756527No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG4450.3451592386330143No Hit
TGTAAAACGACGGCCAGTCCCTGTCCTGAGCACCGTTGCTAACCCCCAGT4300.333524657555497No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT4280.33197338007849464No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGTCATAGCTGTT4280.33197338007849464No Hit
TGTAAAANGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCTCCTTCCCAT4280.33197338007849464No Hit
TGTAAAANGACGGCCAGTTGTAGCCCCCAGGAAGAGGGCGCGGAGGCAGC4230.32809518638598884No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCTGCAGACAGGAT4150.3218900764779796No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCAAGAGGTGGGGT4140.32111443773947845No Hit
TGTAAAANGACGGCCAGTAGGTCATCCTCCTGCCTCGGGTCAGTGTTCCT4110.318787521523975No Hit
TGTAAAANGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG4040.3133580503544669No Hit
TGTAAAANGACGGCCAGTACCTCATGTCACGTGCGTCCACAGTGACCTGT3970.3079285791849588No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTTCCTATCG3880.3009478305384484No Hit
TGTAAAANGACGGCCAGTCAGCCCTGTCACGAATCCCGTGTAAAACGACG3820.2962939981074415No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGCCTGGGTGGGA3780.29319144315343687No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGTCTCCACTCACT3760.2916401656764345No Hit
TGTAAAANGACGGCCAGTGGGGAGTGAGAGGCCATAGCTGTCTGGCATGG3690.2862106945069265No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG3660.283883778291423No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC3610.2800055845989172No Hit
TGTAAAANGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT3600.27922994586041605No Hit
TGTAAAANGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG3580.27767866838341376No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCAGGCCCCAAGGA3580.27767866838341376No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC3570.2769030296449126No Hit
TGTAAAACGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC3470.26914664225990104No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACTGTCCTCTCTCG3470.26914664225990104No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACACTGCCGAGTG3430.2660440873058964No Hit
TGTAAAANGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC3320.2575120611823837No Hit
TGTAAAANGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT3250.25208259001287564No Hit
TGTAAAANGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA3220.24975567379737212No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCCACGCTGGGAAT3200.24820439632036984No Hit
TGTAAAANGACGGCCAGTCCCAGCACAACTTCCCCCATGGCGTGGTGTCC3200.24820439632036984No Hit
TGTAAAANGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC3200.24820439632036984No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCCAGGATGGAGGG3170.24587748010486635No Hit
TGTAAAACGACGGCCAGTGGGGAGTGAGAGGCCATAGCTGTTTCCTGTCA3040.23579417650435136No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCCCTCGGAGCTG2990.23191598281184556No Hit
TGTAAAANGACGGCCAGTCAGCCAGTAGCAACAGCAGCTACTGATGTAAG2920.22648651164233743No Hit
TGTAAAACGACGGCCAGTAGTGACAACAGCCCCAAAGGAATGATCCCGCT2890.224159595426834No Hit
TGTAAAANGACGGCCAGTCCCTGAAGGACAAGCCAGGCAGCAGGATGGAG2830.21950576299582708No Hit
TGTAAAACGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT2720.21097373687231435No Hit
TGTAAAANGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGTCAT2720.21097373687231435No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACTTCCTGCTCCATCCCCGGTC2700.209422459395312No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTGGGCCTGG2640.2047686269643051No Hit
TGTAAAANGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC2630.20399298822580395No Hit
TGTAAAACGACGGCCAGTGGAGTGGCTGCCAGCAAGAGACAGTGTTCAGA2620.2032173494873028No Hit
TGTAAAACGACGGCCAGTTTGACCGTCCCACCTGCCTTGGTTTTCCCTTT2600.2016660720103005No Hit
TGTAAAACGACGGCCAGTACCGGGCCAGGTCAGTTACTGCCAGTACCGGG2530.19623660084079236No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCCGGCCCCAAGGA2470.19158276840978547No Hit
TGTAAAANGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG2470.19158276840978547No Hit
TGTAAAANGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG2450.19003149093278315No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCCATCTGCCGCCC2450.19003149093278315No Hit
TGTAAAACGACGGCCAGTAAGGGTGGGTCATGGGGTGAGGGTGGGTCGCG2420.1877045747172797No Hit
TGTAAAANGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG2400.18615329724027738No Hit
TGTAAAACGACGGCCAGTACCGGGCCAGGTCAGTTACTCCCGACGCCAGG2400.18615329724027738No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG2350.1822751035477716No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGACCCACGAGGGCT2320.1799481873322681No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCATGGGTC2290.17762127111676465No Hit
TGTAAAACGACGGCCAGTCCTGTGCCCCAGCCTCCTTAATGTGTGCACTC2280.1768456323782635No Hit
TGTAAAANGACGGCCAGTGCATGAGGCGAATTCTCAGCCATGACAAGGCT2260.1752943549012612No Hit
TGTAAAANGACGGCCAGTGGAACTGGTGGGCAGTGTCCCAAGAGGTGGGG2240.17374307742425887No Hit
TGTAAAANGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA2230.17296743868575773No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA2160.16753796751624964No Hit
TGTAAAACGACGGCCAGTCCCAGCACAACTTCCCCCATGTAAAACGACGG2120.164435412562245No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCAGTGGGAGTCTGGCTAC2090.16210849634674154No Hit
TGTAAAANGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT2070.16055721886973923No Hit
TGTAAAANGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC2070.16055721886973923No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGTGGGGTCACCCC2020.15667902517723345No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC2010.1559033864387323No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGGCAGCACCTCT1990.15435210896173No Hit
TGTAAAANGACGGCCAGTCCATCCCACAGCCATCCGTGACTGTGACGACA1980.15357647022322884No Hit
TGTAAAANGACGGCCAGTCATAGCCCAGGTCCAGCAGCCACATGCCTCCC1920.1489226377922219No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT1920.1489226377922219No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGAGGCTCCTTCC1870.14504444409971612No Hit
TGTAAAANGACGGCCAGTGGCTCCCCAGCTGTCTCTCTTGTAAAACGACG1830.1419418891457115No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCAGTG1800.13961497293020803No Hit
TGTAAAACGACGGCCAGTAGTACGAAAAGTAGGTGGCCTTACTGGTAGCA1800.13961497293020803No Hit
TGTAAAANGACGGCCAGTCCCTGTCCTGAGCACCGTTGCTAACCCCCAGT1770.13728805671470456No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCGTGGGTC1760.1365124179762034No Hit
TGTAAAACGACGGCCAGTGTTCTACCGTCAGTCCTGGCATTATTTCTCTC1760.1365124179762034No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGTATGGGCTCACT1740.1349611404992011No Hit
TGTAAAANGACGGCCAGTTGAGAAGCTGAGGCTTAAAGAGGGATCCCTTG1710.13263422428369764No Hit
TGTAAAACGACGGCCAGTTTGCTGCTGGGCTTCCTCTGGGCTGCTCTGTC1710.13263422428369764No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG1700.13185858554519647No Hit
TGTAAAANGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC1690.13108294680669533No Hit
TGTAAAACGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCATAAACACAAA1690.13108294680669533No Hit
TGTAAAACGACGGCCAGTGCCACTTCTGAAGGAGGTTCCCCCAGCAGTGC1630.12642911437568838No Hit
TGTAAAACGACGGCCAGTTCATAGCTGTGAAGAGGGATACCAAGGAAAGT1630.12642911437568838No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCAGGATGGAGGGG1610.12487783689868606No Hit
TGTAAAANGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTTCCTATCG1590.12332655942168375No Hit
TGTAAAACGACGGCCAGTTTGCTGCTGGGCTTCCTCTGCCCTGAGGGCTT1590.12332655942168375No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC1570.12177528194468146No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA1550.12022400446767914No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCAGGGGAGGGCTACAGTC1550.12022400446767914No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCACCGAGGCAG1510.11712144951367451No Hit
TGTAAAACGACGGCCAGTCAGTTCCCAGGGACTCTGCTCTATGAGAGAGA1490.1155701720366722No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCCACGCTGGGGAT1480.11479453329817103No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG1460.11324325582116872No Hit
TGTAAAANGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC1450.11246761708266757No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGTGTAAAACGACG1450.11246761708266757No Hit
TGTAAAACGACGGCCAGTGCCCTGAACCGGTGCCAAATGGAGTGAAGAAA1440.11169197834416643No Hit
TGTAAAANGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG1440.11169197834416643No Hit
TGTAAAACGACGGCCAGTCCGCCCTTTCCAAGCTCACTTTAGCCTGGGCA1410.10936506212866295No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCGGTG1410.10936506212866295No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCTGGGGGCTGGCT1400.1085894233901618No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCCGCCCCAAGGAT1390.10781378465166064No Hit
TGTAAAANGACGGCCAGTAAACCTGGCCCCACTGCCTCCTGCTCAGCCTT1390.10781378465166064No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCATAGCTGTT1380.1070381459131595No Hit
TGTAAAANGACGGCCAGTTGATGGACTGGATGCTGCCGTCTCGGCTCACT1370.10626250717465832No Hit
TGTAAAACGACGGCCAGTGGTGTTACCGTGGTCACCCGCAGGGACACGGA1370.10626250717465832No Hit
TGTAAAANGACGGCCAGTGGAACTGGTGGGCAGTGTCCAAGAGGTGGGGT1340.10393559095915486No Hit
TGTAAAANGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC1310.1016086747436514No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCTCAGCCTTCTGGGTC1310.1016086747436514No Hit
TGTAAAACGACGGCCAGTTGTAGCCCCCAGGAAGAGGGTCATAGCTGTTT1300.10083303600515026No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGGGCTCACCATTTGGTG1300.10083303600515026No Hit
TGTAAAACGACGGCCAGTCCACCTGCTTCGCACCATCTCGGCCCAGCCCA1290.10005739726664908No Hit
TGTAAAANGACGGCCAGTTGATGGACTGGATGCTGCCGTCTTGGCTCACT1290.10005739726664908No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)