FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003246740

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246740
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47560
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNCGACATATCCGAGCCTCACGTTCTAGTTTCTG3390.7127838519764509No Hit
NNGGANNNNGNTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG2390.5025231286795627No Hit
NNGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGGACACTGCCCAC1550.32590412111017664No Hit
NNGGANNNNGNTATGNNNGGGGATGGAGCAGGAAGTGACAGGAAACAGCT1370.28805719091673676No Hit
NNGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG1330.2796467619848612No Hit
NNGGANNNNGNTATGNNNTGGAAACCCATGCCATGCACTCAACACTACAA1330.2796467619848612No Hit
NNGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1280.2691337258200168No Hit
NAGGANNNNGNTATGNNNCGACATATCCGAGCCTCACGTTCTAGTTTCTG1250.2628259041211102No Hit
NNGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC1220.25651808242220353No Hit
NNGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA1190.2502102607232969No Hit
NNGGANNNNGNTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC1170.2460050462573591No Hit
NNGGANNNNGNTATGNNNGTCTCAGGGATCACGTGGCGCAGACCTCGCTC1160.24390243902439024No Hit
NNGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT1100.23128679562657695No Hit
NNGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC1050.22077375946173255No Hit
NNGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGACACTGCCCACC1030.21656854499579478No Hit
NNGGANNNNGNTATGNNNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1020.2144659377628259No Hit
NNGGANNNNGNTATGNNNTCCCTTAAACTTGGGATTGCTCCATTGCACTC960.20185029436501262No Hit
NNGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC930.19554247266610597No Hit
NNGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT910.1913372582001682No Hit
NNGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC890.18713204373423045No Hit
NNGGANNNNGNTATGNNNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA890.18713204373423045No Hit
NAGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGGACACTGCCCAC840.17661900756938603No Hit
NNGGANNNNGNTATGNNNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT840.17661900756938603No Hit
NNGGANNNNGNTATGNNNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC840.17661900756938603No Hit
NNGGANNNNGNTATGNNNGGGTCCCACCCATGCTGAAATGTGATCCTCAA830.17451640033641716No Hit
NNGGANNNNGNTATGNNNGGTAGCAACGTCCCACCCAGGGCACGGGAACA820.1724137931034483No Hit
NNGGANNNNGNTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA790.1661059714045416No Hit
NNGGANNNNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT790.1661059714045416No Hit
NNGGANNNNGNTATGNNNCTTGAGCTCCTCGGACACGGCAGGAAACAGCT760.159798149705635No Hit
NNGGANNNNGNTATGANNGTCTCAGGGATCACGTGGCGCAGACCTCGCTC760.159798149705635No Hit
NNGGANNNNGNTATGANNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG760.159798149705635No Hit
NNGGANNNNGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA740.15559293523969722No Hit
NAGGANNNNGNTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG730.15349032800672835No Hit
NNGGANNNNGNTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC730.15349032800672835No Hit
NNGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC730.15349032800672835No Hit
NAGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG720.15138772077375945No Hit
NAGGANNNNGNTATGNNNGTCTCAGGGATCACGTGGCGCAGACCTCGCTC700.1471825063078217No Hit
NAGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA700.1471825063078217No Hit
NNGGANNNNGNTATGNNNGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC700.1471825063078217No Hit
NNGGANNNNGNTATGANNCACGACACCCCACCTCTTGGACACTGCCCACC690.14507989907485283No Hit
NNGGANNNNGNTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT690.14507989907485283No Hit
NNGGANNNNGNTATGANNGGGGATGGAGCAGGAAGTGACAGGAAACAGCT680.14297729184188393No Hit
NAGGANNNNGNTATGNNNTGGAAACCCATGCCATGCACTCAACACTACAA670.14087468460891506No Hit
NAGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT640.1345668629100084No Hit
NAGGANNNNGNTATGNNNGGGGATGGAGCAGGAAGTGACAGGAAACAGCT640.1345668629100084No Hit
NAGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT640.1345668629100084No Hit
NNGGANNNNGNTATGANNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT640.1345668629100084No Hit
NNGGANNNNGNTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA630.1324642556770395No Hit
NNGGANNNNGNTATGNNNTTGGCTGGGGCGACTGACCCTGTCTCTCTAGC620.13036164844407067No Hit
NNGGANNNNGNTATGNNNGGGGATGGAGCAGGAAGTGACAGGGCTGACTG610.12825904121110177No Hit
NNGGANNNNGNTATGNNNAGGCACCAACAGTACAAAGCAGATACAAAGCA600.1261564339781329No Hit
NNGGANNNNGNTATGANNTCCCTTAAACTTGGGATTGCTCCATTGCACTC590.124053826745164No Hit
NNGGANNNNGNTATGNNNTAGAGGACACCCAAGGCCACCGGGGATCCTCG590.124053826745164No Hit
NAGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT590.124053826745164No Hit
NNGGANNNNGNTATGNNNGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA590.124053826745164No Hit
NNGGANNNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC590.124053826745164No Hit
NNGGANNNNGNTATGNNNGCTAATGCTTACTCTTTGCGTGAGTACTGGTT580.12195121951219512No Hit
NNGGANNNNGNTATGNNNACCTTTACCCAAATAGCTACCACCAGTGCCTT570.11984861227922623No Hit
NNGGANNNNGNTATGNNNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA570.11984861227922623No Hit
NAGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC560.11774600504625736No Hit
NNGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC560.11774600504625736No Hit
NNGGANNNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT560.11774600504625736No Hit
NNGGANNNNGNTATGNNNCATGTGCCCCAGTAGAGAGCACAGGAAACAGC560.11774600504625736No Hit
NNGGANNNNGNTATGNNNCAGGGCTCCAAGAATGTTCCACACATCTTTCT560.11774600504625736No Hit
NAGGANNNNGNTATGNNNTGTTTTGCCCCTGAAATGAATATTCATCGTCT550.11564339781328847No Hit
NNGGANNNNGNTATGANNGGGTCCCACCCATGCTGAAATGTGATCCTCAA550.11564339781328847No Hit
NNGGANNNNGNTATGNNNTGACTGAAAGGCTTTTCCACGTGCACTGCTGG550.11564339781328847No Hit
CAGGAAANAGCTATGANNGGGGATGGAGCAGGAAGTGACAGGAAACAGCT540.11354079058031959No Hit
NNGGANNNNGNTATGNNNAGGACCACCCCTCCATCCTGCTGCCTGGCTTG540.11354079058031959No Hit
NAGGAANNNGNTATGANNGGGGATGGAGCAGGAAGTGACAGGAAACAGCT530.11143818334735073No Hit
NAGGANNNNGNTATGNNNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA520.10933557611438184No Hit
NNGGANNNNGNTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA520.10933557611438184No Hit
NNGGAAANNGNTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG510.10723296888141295No Hit
NNGGANNNNGNTATGNNNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT510.10723296888141295No Hit
NNGGANNNNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC500.10513036164844407No Hit
NAGGANNNNGNTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC500.10513036164844407No Hit
CAGGAAANAGCTATGANNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG500.10513036164844407No Hit
NNGGANNNNGNTATGANNGGTAGCAACGTCCCACCCAGGGCACGGGAACA500.10513036164844407No Hit
NAGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC500.10513036164844407No Hit
NNGGANNNNGNTATGNNNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG500.10513036164844407No Hit
NAGGANNNNGNTATGANNGTCTCAGGGATCACGTGGCGCAGACCTCGCTC490.10302775441547518No Hit
NNGGANNNNGNTATGNNNACCCAGTTAGAGCCATGGTTCCTGTAGGCTAG490.10302775441547518No Hit
NNGGANNNNGNTATGNNNCCCAAACCTGCGGAGGCACACAGCTGGGAGGC490.10302775441547518No Hit
NAGGANNNNGNTATGNNNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC480.10092514718250631No Hit
NAGGANNNNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG480.10092514718250631No Hit
NNGGANNNNGNTATGANNCCCAAACCTGCGGAGGCACACAGCTGGGAGGC480.10092514718250631No Hit
NNGGANNNNGNTATGNNNCTCAGCTCTGCAGCCTCCGCACAGACCCCACA480.10092514718250631No Hit
CAGGAAANAGCTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA480.10092514718250631No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)