FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84595
Sequences flagged as poor quality0
Sequence length300
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG13821.6336662923340624No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT12881.5225486139842779No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGCACTCCTTC12191.4409835096636916No Hit
CAGGAAACAGCTATGACCCATCAGGCCCCACCTCCAACCCCGTTAGCTGC11491.3582363023819375No Hit
CAGGAAACAGCTATGACCGTCTCAGGGATCACGTGGCGCAGACCTCGCTC10971.2967669484012057No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC9611.136000945682369No Hit
CAGGAAACAGCTATGACCAGGCAGTAACTTGGGCATTTCTTCTGATTCTC9141.0804421065074767No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA9101.075713694662805No Hit
CAGGAAACAGCTATGACCAGAGGCACAGGTGGAGAGGGACGAGACCCTGA8120.9598676044683492No Hit
CAGGAAANAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG7790.920858206749808No Hit
CAGGAAACAGCTATGACCATCCACTGTTGGCCAACACACCTGGCCTTCAA7490.8853951179147704No Hit
CAGGAAACAGCTATGACCTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC6590.7790058514096577No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT6310.745906968496956No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGCTGCCTGGCTTG6010.7104438796619186No Hit
CAGGAAACAGCTATGACCTGGGGATGAGGACAGCAGGGCAGTCACCATCT5790.6844376145162243No Hit
CAGGAAACAGCTATGACCTCCCTTAAACTTGGGATTGCTCCATTGCACTC5730.6773449967492169No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT5690.6726165849045452No Hit
CAGGAAANAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT5550.6560671434481943No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCTCCCCGCCAAGA5430.6418819079141793No Hit
NAGGAAANAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG5420.6406998049530114No Hit
CAGGAAACAGCTATGACCGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA5420.6406998049530114No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT4990.5898693776227909No Hit
CAGGAAANAGCTATGACCTGGAGTTTGAAACAGCACCTTGGCACTCCTTC4890.5780483480111118No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGTGCACCTCCTTC4870.5756841420887759No Hit
CAGGAAANAGCTATGACCGTCTCAGGGATCACGTGGCGCAGACCTCGCTC4760.5626810095159289No Hit
CAGGAAANAGCTATGACCCATCAGGCCCCACCTCCAACCCCGTTAGCTGC4760.5626810095159289No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA4470.5284000236420592No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTAGGAGGGCTGCG4390.5189431999527159No Hit
CAGGAAACAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT4230.5000295525740291No Hit
CAGGAAANAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA4230.5000295525740291No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCACCCCACCCTGCT4220.49884744961286126No Hit
CAGGAAANAGCTATGACCAGGCAGTAACTTGGGCATTTCTTCTGATTCTC4180.4941190377681896No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC4130.48820852296235No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCTGGGTGCTGGTG4090.48348011111767836No Hit
CAGGAAANAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC3960.46811277262249545No Hit
CAGGAAACAGCTATGACCCCTGGACGCAATCACCCAGGCTGCAGTGCGGT3950.46693066966132746No Hit
CAGGAAACAGCTATGACCCCGTAGCCACGCCCCTATTGTCTGACCACGCT3720.4397423015544654No Hit
CAGGAAACAGCTATGACCGATGCTCCTTCCTTGGCCCCAGGCCCTGGCGT3690.43619599267096165No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGGAGTCAC3340.3948223890300845No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGTCTGGGTCCT3290.38891187422424495No Hit
CAGGAAANAGCTATGACCAGAGGCACAGGTGGAGAGGGACGAGACCCTGA3210.3794550505349016No Hit
CAGGAAANAGCTATGACCTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC3190.37709084461256576No Hit
CAGGAAANAGCTATGACCATCCACTGTTGGCCAACACACCTGGCCTTCAA3180.3759087416513978No Hit
CAGGAAANAGCTATGACCTGGGGATGAGGACAGCAGGGCAGTCACCATCT3140.37118032980672616No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACGGAGATCCAGCC3130.3699982268455583No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACCCTTTGTCCA3080.3640877120397186No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGGCACGGGAACA3070.36290560907855074No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGATACTACTA3040.35935930019504697No Hit
CAGGAAACAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC3010.35581299131154326No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCAAGCCTGACTG2870.3392635498551924No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGACACACCACT2790.32980672616584905No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT2770.3274425202435132No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGGTCCTCCTC2750.3250783143211774No Hit
CAGGAAANAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT2730.3227141083988415No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTCCCCAACCATAG2680.316803593593002No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA2640.3120751817483303No Hit
NAGGAAANAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT2630.3108930787871624No Hit
CAGGAAACAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT2620.30971097582599444No Hit
CAGGAAANAGCTATGACCGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA2590.30616466694249067No Hit
CAGGAAANAGCTATGACCGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT2540.30025415213665113No Hit
NAGGAAANAGCTATGACCTGGAGTTTGAAACAGCACCTTGGCACTCCTTC2490.29434363733081154No Hit
CAGGAAACAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG2490.29434363733081154No Hit
CAGGAAANAGCTATGACCTCCCTTAAACTTGGGATTGCTCCATTGCACTC2450.2896152254861398No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCCTCTCCCAACT2420.2860689166026361No Hit
NAGGAAANAGCTATGACCGTCTCAGGGATCACGTGGCGCAGACCTCGCTC2390.28252260771913235No Hit
CAGGAAANAGCTATGACCACCTCTCTGGGCCTGTCTCCTCCCCGCCAAGA2370.2801584017967965No Hit
CAGGAAANAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT2340.27661209291329275No Hit
NAGGAAANAGCTATGACCCATCAGGCCCCACCTCCAACCCCGTTAGCTGC2240.2647910633016136No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGTCTGCAGCCTGG2200.2600626514569419No Hit
CAGGAAANAGCTATGACCAGGACCACCCCTCCATCCTGCTGCCTGGCTTG2190.25888054849577397No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCGTGTGCGCTGGA2160.25533423961227025No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC2160.25533423961227025No Hit
CAGGAAANAGCTATGACCGCCTCTCAGGGCCTCACAGTAGGAGGGCTGCG2150.2541521366511023No Hit
CAGGAAACAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG2070.24469531296175898No Hit
CAGGAAACAGCTATGACCAGAGGCACAGGTGGAGAGGGGGTGATGGCCAT2030.2399669011170873No Hit
NAGGAAANAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2020.2387847981559194No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACCGGGGATCCTCG2000.23642059223358355No Hit
CAGGAAANAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT1920.22696376854424022No Hit
CAGGAAACAGCTATGACCCATCAGGCCCCACCTCCAACCAGGAAACAGCT1920.22696376854424022No Hit
CAGGAAANAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA1900.2245995626219044No Hit
CAGGAAACAGCTATGACCGGGCCTTCTCGAAGCTCTCCCAGGAAGCGCCC1870.2210532537384006No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGACACTGCAGCCTGGG1870.2210532537384006No Hit
NAGGAAANAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA1870.2210532537384006No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGCCTGGCTTGTC1860.2198711507772327No Hit
CAGGAAANAGCTATGACCAAGGACAAGGTGAGGCAGCACCCCACCCTGCT1840.21750694485489688No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC1840.21750694485489688No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGGGGGCCTGGCT1800.2127785330102252No Hit
CAGGAAACAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA1800.2127785330102252No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGTACC1770.20923222412672143No Hit
CAGGAAANAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC1760.20805012116555355No Hit
CAGGAAANAGCTATGACCATTAGCCAGGTGTGGTGGCGTGCACCTCCTTC1740.2056859152432177No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGAACAAAAAGCCTCAC1740.2056859152432177No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGCTCCTCCTCGC1730.20450381228204975No Hit
CAGGAAANAGCTATGACCCCTGGACGCAATCACCCAGGCTGCAGTGCGGT1730.20450381228204975No Hit
NAGGAAANAGCTATGACCAGAGGCACAGGTGGAGAGGGACGAGACCCTGA1710.20213960635971392No Hit
CAGGAAANAGCTATGACCGATGCTCCTTCCTTGGCCCCAGGCCCTGGCGT1670.19741119451504224No Hit
CAGGAAANAGCTATGACCCCGTAGCCACGCCCCTATTGTCTGACCACGCT1660.19622909155387436No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT1660.19622909155387436No Hit
CAGGAAACAGCTATGACCCCAGGAGAGAGCAGCCTTCCTCACACCCGTGA1640.1938648856315385No Hit
CAGGAAANAGCTATGACCACAGGAGACAGGATTGGGGCTGGGTGCTGGTG1630.1926827826703706No Hit
CAGGAAACAGCTATGACCTCAAGCAAGTTCAGGATGGTATAGAAATAGAG1630.1926827826703706No Hit
CAGGAAACAGCTATGACCGGGCCTTCTCGAAGCTCTCCATGAGGTCACTG1600.18913647378686682No Hit
NAGGAAANAGCTATGACCAGGCAGTAACTTGGGCATTTCTTCTGATTCTC1580.186772267864531No Hit
NAGGAAANAGNTATGANCCGACATATCCGAGCCTCACGTTCTAGTTTCTG1570.18559016490336308No Hit
CAGGAAANAGCTATGACCGCCTCTCAGGGCCTCACAGTGGTCTGGGTCCT1510.17849754713635557No Hit
NAGGAAANAGCTATGACCATCCACTGTTGGCCAACACACCTGGCCTTCAA1490.17613334121401975No Hit
CAGGAAANAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC1480.1749512382528518No Hit
CAGGAAANAGCTATGACCTAGAGGACACCCAAGGCCACGGAGATCCAGCC1440.17022282640818015No Hit
CAGGAAACAGCTATGACCCCTGGACGCAATCACCCAGGCTGGAGGGCAGT1440.17022282640818015No Hit
NAGGAAANAGCTATGACCTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC1420.1678586204858443No Hit
CAGGAAANAGCTATGACCGGTAGCAACGTCCCACCCAGGGCACGGGAACA1420.1678586204858443No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCAGGCAAACTTGG1370.16194810568000473No Hit
NAGGAAANAGCTATGACCGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT1370.16194810568000473No Hit
CAGGAAACAGCTATGACCGGCGGGCCCTGATATAGAGCCACTGGCCGTCG1360.16076600271883681No Hit
NAGGAAANAGCTATGACCAGGACCACCCCTCCATCCTGCTGCCTGGCTTG1360.16076600271883681No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGCCTGCCCAGGCC1350.1595838997576689No Hit
CAGGAAANAGCTATGACCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA1340.158401796796501No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCAGCACAGGGAGT1330.15721969383533307No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCCCTCAGCGGG1320.15603759087416516No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC1310.15485548791299722No Hit
CAGGAAANAGCTATGACCTGCCAAAATTAAGGATGCACACCTGGAGTCAC1280.15130917902949348No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCTCCGTGTGCGCT1270.15012707606832557No Hit
NAGGAAANAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT1260.14894497310715765No Hit
CAGGAAACAGCTATGACCTGGGGATGAGGACAGCAGGGCCCCGGGAGCTG1260.14894497310715765No Hit
NAGGAAANAGCTATGACCACCTCTCTGGGCCTGTCTCCTCCCCGCCAAGA1250.14776287014598974No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGGTGATTCAGTAG1250.14776287014598974No Hit
NAGGAAANAGCTATGACCTGGGGATGAGGACAGCAGGGCAGTCACCATCT1240.1465807671848218No Hit
CAGGAAANAGCTATGACCTGGAGTTTGAAACAGCACCTTGGATACTACTA1210.14303445830131806No Hit
NAGGAAANAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT1210.14303445830131806No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTGTAATCCCAGC1190.14067025237898223No Hit
CAGGAAANAGCTATGACCCGTTAGCTGCCTCTCCTGCCACCCTTTGTCCA1190.14067025237898223No Hit
CAGGAAACAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG1180.1394881494178143No Hit
CAGGAAANAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG1170.13830604645664638No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC1160.13712394349547846No Hit
CAGGAAANAGCTATGACCGCCTCTCAGGGCCTCACAGTGGACACACCACT1160.13712394349547846No Hit
CAGGAAANAGCTATGACCACAGGGGCCTCTTAAAACTGCAAGCCTGACTG1130.13357763461197472No Hit
CAGGAAANAGCTATGACCTGGAGTTTGAAACAGCACCTTGGGTCCTCCTC1130.13357763461197472No Hit
CAGGAAANAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT1120.1323955316508068No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTCTGCAGGTCTC1120.1323955316508068No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA1120.1323955316508068No Hit
NAGGAAANAGCTATGACCTCCCTTAAACTTGGGATTGCTCCATTGCACTC1110.13121342868963887No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGTAGTCTC1100.13003132572847095No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC1100.13003132572847095No Hit
CAGGAAANAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG1080.12766711980613513No Hit
CAGGAAACAGCTATGACCCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT1070.1264850168449672No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGTAGTTCC1060.1253029138837993No Hit
CAGGAAACAGCTATGACCTGGGGATGAGGACAGCAGGGAGCTGCGAGCAT1060.1253029138837993No Hit
NAGGAAANAGCTATGACCGCCTCTCAGGGCCTCACAGTAGGAGGGCTGCG1040.12293870796146344No Hit
CAGGAAACAGCTATGACCGTCTCAGGGATCACGTGGCGGAGAAGCTGCTA1010.1193923990779597No Hit
NAGGAAANAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA1010.1193923990779597No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA1000.11821029611679178No Hit
NAGGAAANAGCTATGACCGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA1000.11821029611679178No Hit
CAGGAAANAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT990.11702819315562386No Hit
CAGGAAANAGCTATGACCTGCTGCCGAGAGAGGACAGTCCCCAACCATAG970.11466398723328802No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCCCGTGTGCGCTG960.11348188427212011No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGTTATCCC960.11348188427212011No Hit
CAGGAAACAGCTATGACCACACATACTCCCTTGCTCCATAGCTGCAGAAC940.11111767834978427No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCCTGCCCCCTGCA940.11111767834978427No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTCACACCTGTAA920.10875347242744844No Hit
CAGGAAANAGCTATGACCAGGACCACCCCTCCATCCTGTCTGCAGCCTGG890.10520716354394469No Hit
NAGGAAANAGCTATGANCCGACATATCCGAGCCTCACGTTCTAGTTTCTG890.10520716354394469No Hit
CAGGAAANAGCTATGACCGGAAGGGGAATGGGAAGGAGCCTCTCCCAACT870.10284295762160885No Hit
CAGGAAANAGCTATGACCACCTCTCTGGGCCTGTCTCCGTGTGCGCTGGA870.10284295762160885No Hit
NAGGAAANAGCTATGACCGATGCTCCTTCCTTGGCCCCAGGCCCTGGCGT870.10284295762160885No Hit
CAGGAAANAGCTATGACCCCAGGAGAGAGCAGCCTTCCTCACACCCGTGA850.10047875169927302No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)