FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83270
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT5750.6905247988471238No Hit
NNGGANNNNGNTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT4250.510387894800048No Hit
NNGGANNNNGNTATGNNNCGACATATCCGAGCCTCACGTTCTAGTTTCTG3570.42872583163204037No Hit
NAGGANNNNGNTATGNNNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT3030.3638765461750931No Hit
NNGGANNNNGNTATGNNNGTCTCAGGGATCACGTGGCGCAGACCTCGCTC2760.33145190344661946No Hit
NNGGANNNNGNTATGNNNTGTTTTGCCCCTGAAATGAATATTCATCGTCT2430.29182178455626273No Hit
NNGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2310.2774108322324967No Hit
NNGGANNNNGNTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG2300.27620991953884955No Hit
NNGGANNNNGNTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT2150.2581962291341419No Hit
NNGGANNNNGNTATGANNGTCTCAGGGATCACGTGGCGCAGACCTCGCTC2120.25459349105320045No Hit
NNGGANNNNGNTATGNNNAACACGGCAGTCAGAGGTGCAGGAAACAGCTA2100.25219166566590606No Hit
NNGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA2010.24138345142308157No Hit
NNGGANNNNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1990.23898162603578718No Hit
NNGGANNNNGNTATGNNNACAGTGTCCCCATCAGGCTCCGGGACAGCATC1970.23657980064849285No Hit
NNGGANNNNGNTATGNNNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1920.230575237180257No Hit
NNGGANNNNGNTATGNNNTGCTTCAGTCGCCTGCACAACAGTGAAGACAG1830.21976702293743244No Hit
NNGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG1790.21496337216284378No Hit
NAGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1770.2125615467755494No Hit
NNGGANNNNGNTATGNNNGGGACCAGTCCAATAACCCCTGAGGGGTATAT1750.21015972138825506No Hit
NNGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1740.20895880869460792No Hit
NNGGANNNNGNTATGNNNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT1740.20895880869460792No Hit
NNGGANNNNGNTATGNNNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC1740.20895880869460792No Hit
NNGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC1730.20775789600096076No Hit
NAGGANNNNGNTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT1670.2005524198390777No Hit
NNGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC1580.18974420559625316No Hit
NNGGANNNNGNTATGNNNTGACTGAAAGGCTTTTCCACGTGCACTGCTGG1570.188543292902606No Hit
NNGGANNNNGNTATGNNNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT1570.188543292902606No Hit
NNGGANNNNGNTATGNNNTGCTGGGAGAGGGGGATGAGTAGACAGGGCCA1570.188543292902606No Hit
NNGGANNNNGNTATGNNNGGGTCCCACCCATGCTGAAATGTGATCCTCAA1520.18253872943437013No Hit
NAGGANNNNGNTATGNNNGTCTCAGGGATCACGTGGCGCAGACCTCGCTC1520.18253872943437013No Hit
NNGGANNNNGNTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC1510.18133781674072294No Hit
NNGGANNNNGNTATGNNNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1470.17653416596613425No Hit
NNGGANNNNGNTATGANNAACACGGCAGTCAGAGGTGCAGGAAACAGCTA1460.1753332532724871No Hit
NNGGANNNNGNTATGNNNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC1450.17413234057883992No Hit
NNGGANNNNGNTATGNNNGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC1440.17293142788519275No Hit
NAGGANNNNGNTATGNNNCGACATATCCGAGCCTCACGTTCTAGTTTCTG1400.16812777711060406No Hit
NNGGANNNNGNTATGNNNTAGAGGACACCCAAGGCCACGGAGATCCAGCC1390.1669268644169569No Hit
NNGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG1380.16572595172330973No Hit
NNGGANNNNGNTATGANNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC1380.16572595172330973No Hit
NNGGANNNNGNTATGANNTGCTTCAGTCGCCTGCACAACAGTGAAGACAG1370.16452503902966256No Hit
NNGGANNNNGNTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1360.16332412633601537No Hit
NNGGANNNNGNTATGNNNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA1350.1621232136423682No Hit
NAGGANNNNGNTATGNNNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1340.160922300948721No Hit
NNGGANNNNGNTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA1310.1573195628677795No Hit
NNGGANNNNGNTATGANNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG1300.15611865017413235No Hit
CAGGAAANAGCTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT1300.15611865017413235No Hit
NNGGANNNNGNTATGANNTGACTGAAAGGCTTTTCCACGTGCACTGCTGG1290.15491773748048518No Hit
NNGGANNNNGNTATGANNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT1270.15251591209319082No Hit
NNGGANNNNGNTATGNNNGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA1270.15251591209319082No Hit
NNGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC1250.1501140867058965No Hit
NAGGAANNNGNTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT1180.14170769785036627No Hit
NNGGANNNNGNTATGNNNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG1180.14170769785036627No Hit
NNGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG1170.1405067851567191No Hit
NAGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA1170.1405067851567191No Hit
NNGGANNNNGNTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT1150.13810495976942477No Hit
NNGGANNNNGNTATGNNNCTGGTCCTCATCCTTGGGGCCTGAGAGCCTGT1140.13690404707577758No Hit
NNGGAAANNGNTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT1120.13450222168848325No Hit
NAGGAAANAGCTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT1120.13450222168848325No Hit
NNGGANNNNGNTATGNNNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1080.12969857091389456No Hit
NNGGANNNNGNTATGANNGGGGAGACTGGGCCATACGATAGGCTTAATCC1080.12969857091389456No Hit
NNGGANNNNGNTATGNNNCTGGGCCCCAAAGCAAAGGAGTAGGTGACGAG1080.12969857091389456No Hit
NNGGANNNNGNTATGNNNCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG1070.1284976582202474No Hit
NNGGANNNNGNTATGANNGGGTCCCACCCATGCTGAAATGTGATCCTCAA1070.1284976582202474No Hit
NAGGANNNNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1070.1284976582202474No Hit
NAGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC1070.1284976582202474No Hit
NNGGANNNNGNTATGNNNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1060.12729674552660022No Hit
NNGGANNNNGNTATGANNGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC1030.12369400744565869No Hit
NAGGANNNNGNTATGNNNAACACGGCAGTCAGAGGTGCAGGAAACAGCTA1030.12369400744565869No Hit
NAGGANNNNGNTATGANNGTCTCAGGGATCACGTGGCGCAGACCTCGCTC1030.12369400744565869No Hit
NNGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCAGGATGTAGTCAG1020.12249309475201153No Hit
NNGGANNNNGNTATGNNNAGCAAAGGTCTTGGCAGGGGCTGGGGACTGGA1020.12249309475201153No Hit
NNGGANNNNGNTATGNNNTCCTGGAACCTGCCTCCGAAGGCCGCAGCTCC1020.12249309475201153No Hit
NNGGANNNNGNTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT1020.12249309475201153No Hit
NAGGANNNNGNTATGNNNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC1020.12249309475201153No Hit
NNGGANNNNGNTATGNNNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1010.12129218205836437No Hit
NAGGANNNNGNTATGNNNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1010.12129218205836437No Hit
NNGGANNNNGNTATGNNNGGGGAGACTGGGCCATACGATAGGCTTAATCC1000.12009126936471717No Hit
NNGGANNNNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1000.12009126936471717No Hit
NAGGANNNNGNTATGNNNTGCTTCAGTCGCCTGCACAACAGTGAAGACAG990.11889035667107001No Hit
NNGGANNNNGNTATGANNTCCTGCCCCACCCTGATACAGGTCACTGTGGA990.11889035667107001No Hit
NAGGANNNNGNTATGNNNACAGTGTCCCCATCAGGCTCCGGGACAGCATC990.11889035667107001No Hit
NNGGANNNNGNTATGNNNGGGAGGTGGAGGTTGCAGTGAGCCGAGACGGC980.11768944397742284No Hit
NNGGANNNNGNTATGANNTGCTGGGAGAGGGGGATGAGTAGACAGGGCCA980.11768944397742284No Hit
NAGGANNNNGNTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG950.11408670589648133No Hit
NNGGANNNNGNTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC940.11288579320283415No Hit
NNGGANNNNGNTATGNNNTCCTGCCCCACCCTGATACAGGTCACTGTGGA940.11288579320283415No Hit
NNGGANNNNGNTATGNNNAGGACCACCCCTCCATCCTGCTGCCTGGCTTG940.11288579320283415No Hit
NNGGANNNNGNTATGNNNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA940.11288579320283415No Hit
NAGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG940.11288579320283415No Hit
NNGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA930.11168488050918698No Hit
NNGGANNNNGNTATGANNCTGGTCCTCATCCTTGGGGCCTGAGAGCCTGT930.11168488050918698No Hit
NNGGANNNNGNTATGNNNTAGAGGACACCCAAGGCCACCCGTCCTCGGAC930.11168488050918698No Hit
NNGGANNNNGNTATGANNAGCAAAGGTCTTGGCAGGGGCTGGGGACTGGA930.11168488050918698No Hit
NNGGANNNNGNTATGNNNTAGAGGACACCCAAGGCCACCGGGGATCCTCG920.11048396781553982No Hit
NNGGANNNNGNTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG920.11048396781553982No Hit
NAGGANNNNGNTATGNNNTGACTGAAAGGCTTTTCCACGTGCACTGCTGG910.10928305512189264No Hit
NNGGANNNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC910.10928305512189264No Hit
NNGGANNNNGNTATGANNCTGGGCCCCAAAGCAAAGGAGTAGGTGACGAG890.10688122973459831No Hit
NNGGANNNNGNTATGANNAGGACCACCCCTCCATCCTGCTGCCTGGCTTG890.10688122973459831No Hit
NAGGANNNNGNTATGNNNGGGTCCCACCCATGCTGAAATGTGATCCTCAA890.10688122973459831No Hit
NAGGAAANAGNTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT880.10568031704095111No Hit
NNGGANANAGNTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT880.10568031704095111No Hit
NNGGANNNNGNTATGANNTCCTGGAACCTGCCTCCGAAGGCCGCAGCTCC880.10568031704095111No Hit
NAGGANNNNGNTATGNNNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC870.10447940434730396No Hit
NNGGANNNNGNTATGNNNGGGAGGTGGAGGTTGCAGTGAGTGGAGACGGC870.10447940434730396No Hit
NNGGANNNNGNTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA860.10327849165365677No Hit
NNGGANNNNGNTATGANNGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA850.1020775789600096No Hit
NAGGANNNNGNTATGANNTGCTTCAGTCGCCTGCACAACAGTGAAGACAG850.1020775789600096No Hit
NAGGANNNNGNTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT850.1020775789600096No Hit
NAGGANNNNGNTATGNNNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT840.10087666626636244No Hit
NNGGANNNNGNTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA840.10087666626636244No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)