FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70775
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG39365.5612857647474385No Hit
CAGGAAANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG14182.0035323207347226No Hit
CAGGAAANAGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC9921.4016248675379726No Hit
CAGGAAANAGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT8951.264570823030731No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC7901.1162133521723774No Hit
CAGGAAANAGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG6970.9848110208406924No Hit
CAGGAAANAGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT6110.8632991875662309No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT5590.7898269162839986No Hit
CAGGAAANAGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC4680.6612504415400918No Hit
CAGGAAANAGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA4440.6273401624867537No Hit
CAGGAAANNGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC4320.6103850229600848No Hit
NAGGAAANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG3880.5482161780289649No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA3850.5439773931472978No Hit
CAGGANANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG3760.531261038502296No Hit
CAGGAAANNGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT3750.5298481102084069No Hit
CAGGAAANAGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG3530.498763687742847No Hit
NAGGANANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG3470.49028611797951255No Hit
CAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC3460.48887318968562343No Hit
CAGGAAANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG3360.4747439067467326No Hit
CAGGAAANAGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG3350.4733309784528435No Hit
CAGGAAANAGCTATGANNCAGGGTGACTCCGATCCTGGAGGACAAGGCGG3070.4337689862239491No Hit
CAGGAAANAGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA3030.4281172730483928No Hit
CAGGAAANAGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC3020.42670434475450375No Hit
CAGGAAANAGCTATGANNGGGGAGACTGGGCCATACGATAGGCTTAATCC2890.4083362769339456No Hit
CAGGAAANAGCTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT2870.4055104203461674No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA2860.4040974920522783No Hit
CAGGAAANAGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA2830.3998587071706111No Hit
CAGGAAANNGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT2680.37866478276227483No Hit
CAGGAAANAGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA2360.3334510773578241No Hit
CAGGAAACAGCNATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG2280.3221476510067114No Hit
CAGGAAACAGCTATGANCCGACATATCCGAGCCTCACGTTCTAGTTTCTG2230.315083009537266No Hit
CAGGAAANAGCTATGANNTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG2220.31367008124337686No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA2220.31367008124337686No Hit
NAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG2170.3066054397739315No Hit
CAGGAAACNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG2160.3051925114800424No Hit
CAGGAAANAGCTATGANNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT2150.3037795831861533No Hit
CAGGAAANAGCTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA2100.29671494171670787No Hit
NAGGAAANAGCTATGANCCGACATATCCGAGCCTCACGTTCTAGTTTCTG2100.29671494171670787No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA2090.29530201342281875No Hit
CAGGAAANAGCTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA2000.282585658777817No Hit
CAGGAAANAGCTATGANNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG1980.2797598021900389No Hit
CAGGAAANNGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT1940.27410808901448247No Hit
CAGGAAANAGCTATGANNCCCAAACCTGCGGAGGCACGGGGATGGCAGTG1840.2599788060755917No Hit
CAGGAANNNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG1790.25291416460614624No Hit
CAGGAAANNGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA1730.24443659484281172No Hit
CAGGAAANNGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC1660.2345460967855881No Hit
CAGGAAANAGCTATGANNCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT1630.23030731190392087No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC1620.22889438361003178No Hit
CAGGAAANNGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG1620.22889438361003178No Hit
CAGGAAANNGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG1610.22748145531614272No Hit
CAGGAAANAGCTATGANNCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC1420.20063581773225012No Hit
CAGGAAANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC1400.1978099611444719No Hit
CAGGAAANAGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGTACC1400.1978099611444719No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC1350.19074531967502648No Hit
CAGGAAANAGCTATGANNTTGGACAGCACCTTGAATGCTTTCAGGGAAGC1340.18933239138113742No Hit
CAGGAAANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA1330.18791946308724833No Hit
CAGGAAANAGCTATGANNTTGGACAGCACCTTGAATGCTATCAGGGAAGC1320.1865065347933592No Hit
CAGGAAANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA1290.18226774991169198No Hit
CAGGAAANAGCTATGANNTGCTTCAGTCGCCTGCACAACAGGAAACAGCA1280.1808548216178029No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC1280.1808548216178029No Hit
CAGGAAANAGCTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT1280.1808548216178029No Hit
CAGGAAANNGCTATGANNGGGGAGACTGGGCCATACGATAGGCTTAATCC1260.17802896503002472No Hit
CAGGAAANAGCTATGANNGCAAAACACCTTAACACTCGGCTTCTATTTGC1250.17661603673613563No Hit
CAGGAAANAGCTATGANNCATGTGCCCCAGTAGAGAGCAATTAAATTCCT1240.17520310844224657No Hit
CAGGAAANNGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1240.17520310844224657No Hit
CAGGAAANNGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1230.17379018014835745No Hit
CAGGAAANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA1180.16672553867891204No Hit
CAGGAAANNGCTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT1180.16672553867891204No Hit
CAGGAAANAGCTATGANNCCAGGAGAGAGCAGCCTTCCTCACACCCGTGA1160.16389968209113387No Hit
CAGGAAANAGCTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA1130.15966089720946663No Hit
CAGGAAANNGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA1120.15824796891557752No Hit
CAGGAAANAGCTATGANNTGAAGTGGCTCCATCCTTCCATTTGTTTATAT1120.15824796891557752No Hit
CAGGAAANAGCTATGANNAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT1110.15683504062168843No Hit
CAGGAAANAGCTATGANNTTCATACAGAGATACAAAGGCAACTATGTGCA1090.15400918403391028No Hit
CAGGAAANNGCTATGANNCAGGGTGACTCCGATCCTGGAGGACAAGGCGG1000.1412928293889085No Hit
CAGGAAACNGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT1000.1412928293889085No Hit
CAGGAAACAGCTATGANCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT980.13846697280113035No Hit
CAGGAAANAGCTATGANNTGCCCAGTTGTTTCCAGAACCTTGTTTTTGAC950.1342281879194631No Hit
CAGGAAACAGCTATGANCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG940.132815259625574No Hit
CAGGAAACAGCTATGANCGGGACCAGTCCAATAACCCCTGAGGGGTATAT910.12857647474390674No Hit
CAGGAAANNGCTATGANNCCCAAACCTGCGGAGGCACGGGGATGGCAGTG900.12716354645001765No Hit
CAGGAAANAGCTATGANNGGCAGACAGCCTCTCCTCCTTTCATCACAAAA890.1257506181561286No Hit
CAGGAAANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA880.12433768986223949No Hit
CAGGAAACAGCTATGANCCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC860.12151183327446131No Hit
CAGGAAACNGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT850.12009890498057224No Hit
CAGGAAANAGCTATGANNTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC830.11727304839279405No Hit
CAGGAAANAGCTATGANNGTGCTCCTTGGGGACGTAGTCGCCTGGGGACT830.11727304839279405No Hit
CAGGAAANNGCTATGANNTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG830.11727304839279405No Hit
CAGGAAACNGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC830.11727304839279405No Hit
CAGGAAANAGCTATGANNACCTGGCAGCCCCAAATCTGCACCTCCCGCGG830.11727304839279405No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATTGC820.11586012009890498No Hit
CAGGAAANAGCTATGANNGCTTCTCGGCCATGTGGGTTAAACTCGGGGAT810.11444719180501589No Hit
CAGGAAACNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG800.11303426351112682No Hit
CAGGAAANNGCTATGANNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT800.11303426351112682No Hit
CAGGAAACAGCTATGANCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC790.11162133521723772No Hit
CAGGAAANNGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC780.11020840692334864No Hit
CAGGAAANNGCTATGANNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG780.11020840692334864No Hit
CAGGAAANNGCTATGANNTTGGACAGCACCTTGAATGCTTTCAGGGAAGC770.10879547862945955No Hit
NAGGAAANNGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC750.1059696220416814No Hit
CAGGAAANAGCTATGANNTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC750.1059696220416814No Hit
CAGGAAANNGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA740.10455669374779229No Hit
CAGGAAACNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC730.10314376545390322No Hit
CAGGAANNNGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT720.10173083716001413No Hit
CAGGAAANNGCTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA720.10173083716001413No Hit
CAGGAAANAGCTATGANNGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC720.10173083716001413No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)