FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003246758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47093
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT2480.5266175440086637No Hit
NNGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA1660.35249400123160557No Hit
NNGGANNNNGNTATGNNNTGGAGTTTGAAACAGCACCTTGGCACTCCTTC1580.33550633852164863No Hit
NNGGANNNNGNTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT1550.3291359650054148No Hit
NNGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC1500.3185186758116918No Hit
NNGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT1440.3057779287792241No Hit
NNGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1300.27604951903679953No Hit
NNGGANNNNGNTATGNNNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT1250.26543222984307646No Hit
NAGGANNNNGNTATGNNNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT1210.256938398488098No Hit
NNGGANNNNGNTATGNNNTGGAAACCCATGCCATGCACTCAACACTACAA1210.256938398488098No Hit
NNGGANNNNGNTATGANNTGGAGTTTGAAACAGCACCTTGGCACTCCTTC1200.2548149406493534No Hit
NNGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAACT1180.2505680249718642No Hit
NNGGANNNNGNTATGNNNTCCCTTAAACTTGGGATTGCTCCATTGCACTC1140.24207419361688573No Hit
NNGGANNNNGNTATGNNNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1130.23995073577814113No Hit
NAGGAAANAGCTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT1020.2165926995519504No Hit
NNGGANNNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT980.20809886819697196No Hit
NNGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAATT950.2017284946807381No Hit
NNGGANNNNGNTATGNNNAGGCACCAACAGTACAAAGCAGATACAAAGCA950.2017284946807381No Hit
NNGGANNNNGNTATGNNNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC940.1996050368419935No Hit
NNGGANNNNGNTATGNNNTGCTGCCGAGAGAGGACAGTCCCCAACCATAG920.1953581211645043No Hit
NNGGANNNNGNTATGNNNGGGACCAGTCCAATAACCCCTGAGGGGTATAT920.1953581211645043No Hit
NAGGANNNNGNTATGNNNTGGAGTTTGAAACAGCACCTTGGCACTCCTTC890.18898774764827045No Hit
NNGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC850.180493916293292No Hit
NNGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC850.180493916293292No Hit
NNGGANNNNGNTATGNNNGGTAGCAACGTCCCACCCAGGCTCCTCCTCGC840.17837045845454738No Hit
NNGGAAANNGNTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT840.17837045845454738No Hit
NNGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT830.17624700061580278No Hit
NNGGANNNNGNTATGANNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT830.17624700061580278No Hit
NNGGANNNNGNTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA810.17200008493831354No Hit
NAGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA810.17200008493831354No Hit
NAGGAANNNGNTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT800.16987662709956894No Hit
NAGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC790.16775316926082431No Hit
NAGGANNNNGNTATGNNNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA750.1592593379058459No Hit
NNGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT740.15713588006710127No Hit
NAGGANNNNGNTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT730.15501242222835665No Hit
NAGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT730.15501242222835665No Hit
NNGGANNNNGNTATGNNNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT720.15288896438961205No Hit
CAGGAAANAGCTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT720.15288896438961205No Hit
NAGGAAANAGCTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA710.15076550655086743No Hit
NNGGANNNNGNTATGNNNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA700.1486420487121228No Hit
NNGGANNNNGNTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA700.1486420487121228No Hit
NAGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT690.1465185908733782No Hit
NAGGAAANAGCTATGANNTGGAGTTTGAAACAGCACCTTGGCACTCCTTC680.14439513303463358No Hit
NAGGAAANAGCTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC670.14227167519588899No Hit
NNGGANNNNGNTATGANNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT650.13802475951839976No Hit
NAGGANNNNGNTATGNNNTGGAAACCCATGCCATGCACTCAACACTACAA640.13590130167965514No Hit
NAGGAANNNGNTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA640.13590130167965514No Hit
NNGGANNNNGNTATGNNNGGTAGCAACGTCCCACCCAGCCTGCCCAGGCC630.13377784384091054No Hit
NNGGANNNNGNTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT620.13165438600216592No Hit
NNGGANNNNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC610.12953092816342132No Hit
NNGGANNNNGNTATGNNNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT610.12953092816342132No Hit
CAGGAAANAGCTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA600.1274074703246767No Hit
NNGGANANAGNTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT590.1252840124859321No Hit
NNGGAAANNGNTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA590.1252840124859321No Hit
NAGGANNNNGNTATGNNNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT590.1252840124859321No Hit
CAGGAAANAGCTATGANNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT590.1252840124859321No Hit
NNGGANNNNGNTATGNNNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG580.12316055464718749No Hit
CAGGAAANAGCTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA580.12316055464718749No Hit
NNGGANNNNGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA580.12316055464718749No Hit
NNGGANNNNGNTATGANNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC580.12316055464718749No Hit
NAGGAANNNGNTATGANNTGGAGTTTGAAACAGCACCTTGGCACTCCTTC570.12103709680844287No Hit
NNGGANNNNGNTATGNNNCAGGGCTCCAAGAATGTTCCACACATCTTTCT570.12103709680844287No Hit
NNGGANNNNGNTATGNNNCCTGGACGCAATCACCCAGGCTGCAGTGCGGT560.11891363896969825No Hit
NNGGANNNNGNTATGNNNTGGAGTTTGAAACAGCACCTTGGATACTACTA560.11891363896969825No Hit
NNGGANNNNGNTATGNNNTCCACAAGGCCCTGGGACAAGGAAAATGGAGA560.11891363896969825No Hit
NNGGANNNNGNTATGNNNAAGGACAAGGTGAGGCAGCACCCCACCCTGCT550.11679018113095366No Hit
NNGGAAANNGNTATGANNTGGAGTTTGAAACAGCACCTTGGCACTCCTTC550.11679018113095366No Hit
NNGGANNNNGNTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA540.11466672329220903No Hit
NNGGANNNNGNTATGNNNCTCAGCTCTGCAGCCTCCGCACAGACCCCACA540.11466672329220903No Hit
NNGGANNNNGNTATGNNNACACCCGGAGCAGTGCAAATCAACTCCCCCAG540.11466672329220903No Hit
NAGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAACT540.11466672329220903No Hit
NNGGANNNNGNTATGNNNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG530.11254326545346441No Hit
NNGGANNNNGNTATGNNNGGTAGCAACGTCCCACCCAGGGCACGGGAACA530.11254326545346441No Hit
NAGGAAANAGNTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT530.11254326545346441No Hit
CAGGAAANAGCTATGANNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT520.11041980761471981No Hit
NNGGANNNNGNTATGNNNCTGGTCCTCATCCTTGGGGCCTGAGAGCCTGT520.11041980761471981No Hit
NAGGAAANAGCTATGANNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT520.11041980761471981No Hit
NNGGANNNNGNTATGANNTCCCTTAAACTTGGGATTGCTCCATTGCACTC510.1082963497759752No Hit
NNGGANNNNGNTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT510.1082963497759752No Hit
NNGGANANAGNTATGANNTGGAGTTTGAAACAGCACCTTGGCACTCCTTC510.1082963497759752No Hit
CAGGAAANAGCTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC510.1082963497759752No Hit
NNGGANNNNGNTATGNNNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA500.10617289193723058No Hit
NNGGANNNNGNTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG500.10617289193723058No Hit
NNGGANNNNGNTATGNNNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT500.10617289193723058No Hit
NNGGANNNNGNTATGNNNCATGGTTGGGGAAAAACTCCTCATCTTCATGT500.10617289193723058No Hit
NNGGAAANNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT500.10617289193723058No Hit
NAGGANNNNGNTATGNNNTCCCTTAAACTTGGGATTGCTCCATTGCACTC500.10617289193723058No Hit
NNGGANNNNGNTATGNNNCATGGTTGGGGAAAAACTCCTCATTTTCATGT500.10617289193723058No Hit
NNGGANNNNGNTATGNNNACCTTTACCCAAATAGCTACCACCAGTGCCTT490.10404943409848598No Hit
NNGGANNNNGNTATGNNNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA490.10404943409848598No Hit
NNGGANNNNGNTATGANNCATGGTTGGGGAAAAACTCCTCATTTTCATGT490.10404943409848598No Hit
NAGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC490.10404943409848598No Hit
NAGGAAANAGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT490.10404943409848598No Hit
NNGGAAANNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC490.10404943409848598No Hit
NNGGANANAGNTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA490.10404943409848598No Hit
NNGGAAANNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT480.10192597625974135No Hit
NAGGANNNNGNTATGANNTGGAGTTTGAAACAGCACCTTGGCACTCCTTC480.10192597625974135No Hit
NNGGANNNNGNTATGNNNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA480.10192597625974135No Hit
NNGGANNNNGNTATGNNNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT480.10192597625974135No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)