FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117394
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT6860.5843569518033289No Hit
NNGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC5740.4889517351823773No Hit
NNGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA5660.48213707685230933No Hit
NNGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT5400.45998943727958835No Hit
NNGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT5320.45317477894952046No Hit
NNGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAACT4920.41910148729918056No Hit
NNGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAATT4830.41143499667785405No Hit
NNGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT4820.4105831643865956No Hit
NNGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC4440.37821353731877266No Hit
NNGGANNNNGNTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA4410.3756580404449972No Hit
NNGGANNNNGNTATGNNNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT4290.36543605294989523No Hit
NNGGANNNNGNTATGANNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT3820.32539993526074584No Hit
NNGGANNNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT3760.32028894151319487No Hit
NNGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT3590.3058077925618004No Hit
NNGGANNNNGNTATGNNNTGGGGATGAGGACAGCAGGGCAGTCACCATCT3560.3032522956880249No Hit
NNGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT3450.2938821404841815No Hit
NNGGANNNNGNTATGNNNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT3380.287919314445372No Hit
NNGGANNNNGNTATGNNNAGGCACCAACAGTACAAAGCAGATACAAAGCA3160.2691790040376851No Hit
NNGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC3100.2640680102901341No Hit
NNGGANNNNGNTATGNNNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA3060.2606606811251001No Hit
NNGGANNNNGNTATGNNNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC3030.25810518425132456No Hit
NAGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2970.2529941905037736No Hit
NNGGANNNNGNTATGANNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT2960.2521423582125151No Hit
NNGGANNNNGNTATGNNNGTCTCAGGGATCACGTGGCGCAGACCTCGCTC2950.2512905259212566No Hit
NNGGANNNNGNTATGNNNAGTGGGAGGAGCTCTCGGTCACGGGCATCCTG2940.2504386936299981No Hit
NAGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC2750.2342538800960867No Hit
NNGGANNNNGNTATGNNNTAGAGGACACCCAAGGCCACGGAGATCCAGCC2660.22658738947476023No Hit
NNGGANNNNGNTATGNNNTCCCTTAAACTTGGGATTGCTCCATTGCACTC2620.2231800603097262No Hit
NNGGANNNNGNTATGNNNGGTAGCAACGTCCCACCCAGGCTCCTCCTCGC2620.2231800603097262No Hit
NNGGANNNNGNTATGANNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT2600.2214763957272092No Hit
NNGGANNNNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2540.21636540197965826No Hit
NNGGANNNNGNTATGNNNTCCTGGAACCTGCCTCCGAAGGCCGCAGCTCC2530.21551356968839974No Hit
NNGGANNNNGNTATGNNNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC2440.20784707906707328No Hit
NNGGANNNNGNTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT2430.20699524677581477No Hit
NNGGANNNNGNTATGNNNGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA2330.1984769238632298No Hit
NNGGANNNNGNTATGANNAGTGGGAGGAGCTCTCGGTCACGGGCATCCTG2330.1984769238632298No Hit
NNGGANNNNGNTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA2260.1925140978244203No Hit
NAGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT2210.18825493636812785No Hit
NAGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA2200.18740310407686936No Hit
NNGGANNNNGNTATGNNNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG2160.18399577491183536No Hit
NAGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT2110.17973661345554287No Hit
NNGGANNNNGNTATGANNTCCCTTAAACTTGGGATTGCTCCATTGCACTC2080.17718111658176738No Hit
NNGGANNNNGNTATGNNNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC2070.1763292842905089No Hit
NNGGANNNNGNTATGNNNTAGAGGACACCCAAGGCCACCCGTCCTCGGAC2050.1746256197079919No Hit
NNGGANNNNGNTATGNNNCCCGTGACAGCTCCTGCAATTACAAAATGGCT2040.17377378741673338No Hit
NNGGANNNNGNTATGANNGTCTCAGGGATCACGTGGCGCAGACCTCGCTC1950.16610729679540692No Hit
NNGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC1930.16440363221288992No Hit
NAGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1930.16440363221288992No Hit
NNGGANNNNGNTATGNNNCATGGTTGGGGAAAAACTCCTCATCTTCATGT1920.16355179992163144No Hit
NNGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG1910.16269996763037292No Hit
NAGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC1890.16099630304785595No Hit
NNGGANNNNGNTATGANNGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA1890.16099630304785595No Hit
NNGGANNNNGNTATGNNNATTAGCCAGGTGTGGTGGCGTGCACCTCCTTC1870.15929263846533895No Hit
NAGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAACT1870.15929263846533895No Hit
NNGGANNNNGNTATGNNNCATGGTTGGGGAAAAACTCCTCATTTTCATGT1860.15844080617408046No Hit
NNGGANNNNGNTATGNNNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT1860.15844080617408046No Hit
NNGGANNNNGNTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1860.15844080617408046No Hit
NNGGANNNNGNTATGNNNGCTGCTGACCCAACCCTACCTCTGCAGCTAGC1850.15758897388282195No Hit
NNGGANNNNGNTATGANNGGTAGCAACGTCCCACCCAGGCTCCTCCTCGC1840.15673714159156346No Hit
NNGGANNNNGNTATGNNNAGGGCAAGTGTTCGCGTTGTGACAGTTACGGT1810.15418164471778797No Hit
NNGGANNNNGNTATGANNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC1790.15247798013527097No Hit
NAGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAATT1790.15247798013527097No Hit
NNGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT1780.15162614784401246No Hit
NNGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC1770.15077431555275397No Hit
NNGGANNNNGNTATGANNTAGAGGACACCCAAGGCCACGGAGATCCAGCC1750.14907065097023697No Hit
NNGGANNNNGNTATGNNNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA1690.143959657222686No Hit
NNGGANNNNGNTATGANNTCCTGGAACCTGCCTCCGAAGGCCGCAGCTCC1690.143959657222686No Hit
NAGGANNNNGNTATGNNNAGTGGGAGGAGCTCTCGGTCACGGGCATCCTG1690.143959657222686No Hit
NAGGANNNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1680.1431078249314275No Hit
NNGGANNNNGNTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC1670.142255992640169No Hit
NNGGANNNNGNTATGNNNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1660.1414041603489105No Hit
NNGGANNNNGNTATGNNNGGGGAGACTGGGCCATACGATAGGCTTAATCC1630.13884866347513503No Hit
NAGGANNNNGNTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA1600.1362931666013595No Hit
NAGGANNNNGNTATGNNNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT1540.13118217285380854No Hit
NNGGANNNNGNTATGANNATTAGCCAGGTGTGGTGGCGTGCACCTCCTTC1540.13118217285380854No Hit
NNGGANNNNGNTATGANNTAGAGGACACCCAAGGCCACCCGTCCTCGGAC1530.13033034056255005No Hit
NAGGANNNNGNTATGNNNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT1530.13033034056255005No Hit
NNGGANNNNGNTATGANNCCCGTGACAGCTCCTGCAATTACAAAATGGCT1530.13033034056255005No Hit
NNGGANNNNGNTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1520.12947850827129154No Hit
NNGGANNNNGNTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC1500.12777484368877456No Hit
NAGGANNNNGNTATGANNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT1490.12692301139751605No Hit
NNGGANNNNGNTATGNNNTCCCACCACTGACTCCAGGGGAAGGAGGAGGA1440.12266384994122356No Hit
NNGGANNNNGNTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT1440.12266384994122356No Hit
NNGGANNNNGNTATGNNNCTGGTCCTCATCCTTGGGGCCGGAGAGCCTGT1430.12181201764996506No Hit
NNGGANNNNGNTATGNNNTCCACAAGGCCCTGGGACAAGGAAAATGGAGA1420.12096018535870659No Hit
NAGGANNNNGNTATGNNNGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA1420.12096018535870659No Hit
NNGGANNNNGNTATGNNNACACCCGGAGCAGTGCAAATCAACTCCCCCAG1410.12010835306744809No Hit
NNGGANNNNGNTATGNNNCTGGTCCTCATCCTTGGGGCCTGAGAGCCTGT1380.1175528561936726No Hit
NNGGANNNNGNTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT1350.11499735931989709No Hit
NNGGANNNNGNTATGNNNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT1340.11414552702863859No Hit
NNGGANNNNGNTATGNNNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1330.11329369473738012No Hit
NAGGANNNNGNTATGANNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT1320.11244186244612162No Hit
NNGGANNNNGNTATGNNNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA1280.10903453328108763No Hit
NNGGANNNNGNTATGNNNGGTAGCAACGTCCCACCCAGGGCACGGGAACA1270.10818270098982913No Hit
NNGGANNNNGNTATGANNGCTGCTGACCCAACCCTACCTCTGCAGCTAGC1270.10818270098982913No Hit
NNGGANNNNGNTATGANNCTGGTCCTCATCCTTGGGGCCGGAGAGCCTGT1260.10733086869857061No Hit
NNGGANNNNGNTATGANNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA1260.10733086869857061No Hit
NNGGANNNNGNTATGANNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG1260.10733086869857061No Hit
NNGGANNNNGNTATGNNNTGACAGCACAAGGAACACTGACCCGAGGCAGG1260.10733086869857061No Hit
NAGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT1250.10647903640731211No Hit
NNGGANNNNGNTATGANNAGGGCAAGTGTTCGCGTTGTGACAGTTACGGT1240.10562720411605364No Hit
NAGGANNNNGNTATGNNNAGGCACCAACAGTACAAAGCAGATACAAAGCA1220.10392353953353664No Hit
NNGGANNNNGNTATGNNNTCCTGGAACCTGCCTCCGAAGGCCCCATCCGG1190.10136804265976115No Hit
NAGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT1190.10136804265976115No Hit
NNGGANNNNGNTATGANNGGGGAGACTGGGCCATACGATAGGCTTAATCC1180.10051621036850265No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)