FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109968
Sequences flagged as poor quality0
Sequence length300
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA38563.506474610795868No Hit
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA35563.2336679761385128No Hit
TGTAAAACGACGGCCAGTTCCCAAAGGCTGGTGGAGGAGATGCTGAAGAT32042.91357485814055No Hit
TGTAAAACGACGGCCAGTCCAGAGATCAATAATAGCTCAGGGGAGATGGA29972.725338280226975No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG26812.4379819583878946No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT26382.398879674087007No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA24582.235195693292594No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG22402.036956205441583No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC21401.946020660555798No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC20341.849628982976866No Hit
TGTAAAACGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA19411.765058926233086No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT17741.613196566273825No Hit
TGTAAAACGACGGCCAGTTTGATGCAAGAAAAAGAAACTGGACTTAATGT16611.510439400552888No Hit
TGTAAAACGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT15791.4358722537465445No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCATGGGTC15111.3740360832242107No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG13261.2058053251855085No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG12891.172159173577768No Hit
TGTAAAACGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT12701.154881420049469No Hit
TGTAAAACGACGGCCAGTGGAGTGGCTGCCAGCAAGAGACAGTGTTCAGA12651.1503346428051797No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT11791.0721300742034046No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG11671.0612178088171105No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA11191.0175687472719337No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG11111.010293903681071No Hit
TGTAAAACGACGGCCAGTACACATTCTTGTAAAGCATGGGATTGCTGAAT10530.9575512876473156No Hit
TGTAAAACGACGGCCAGTAGGTGAAGAAGAAAAGACAAAGACCTACAGTG9830.8938964062272662No Hit
TGTAAAACGACGGCCAGTTGACACAGCTGCTTCTGAGTACAAGTATGTAG9520.8657063873126728No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC9410.8557034773752364No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC9310.8466099228866579No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC9180.8347883020515059No Hit
TGTAAAACGACGGCCAGTTCCTGGGCTTCTCTTGAGTACGGAGGAAAGTG9070.8247853921140696No Hit
TGTAAAACGACGGCCAGTTACAGGCCCCACACTGCTGACTCTGCTCCCCT8910.8102357049323439No Hit
TGTAAAACGACGGCCAGTCAGTTCCCAGGGACTCTGCTCTATGAGAGAGA8770.7975047286483341No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC8520.7747708424268879No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCAGTG8390.7629492215917357No Hit
TGTAAAACGACGGCCAGTAGGTCATCCTCCTGCCTCGGCCTCCCAAAGTG8270.7520369562054416No Hit
TGTAAAACGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC7840.7129346719045541No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCGGTG7600.6911101411319657No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTGGTGGTCC7210.6556452786265096No Hit
TGTAAAACGACGGCCAGTTCTTCACCTCCCAAAATGCTGGGATTACAGGC7200.6547359231776516No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC6700.6092681507347592No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA6410.5828968427178816No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG6060.5510694020078568No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC5270.4792303215480867No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG5250.4774116106503711No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC5200.47286483340608176No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTCCCTTCTCTTTG5150.4683180561617925No Hit
TGTAAAACGACGGCCAGTTGAAGAATGGTGGCTTGTTTCATGGTGGCCTT4560.41466608467917937No Hit
TGTAAAACGACGGCCAGTTCCATTACTCTACACAAGCAGGGTACACTTAG4470.40648188563945875No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG4440.4037538192928852No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACCAACGTGGTGCA4410.40102575294631165No Hit
TGTAAAACGACGGCCAGTTGGAACCATTTTTGATAACTTTCTGATCACAG4400.4001163974974538No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGATATCTCCTGCAC4050.36828895678742907No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGCGCGGGCAC4010.36465153499199765No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGAGCTGCACCG3970.3610141131965663No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGTCCCAGCTACT3860.35101120325912993No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCTGCAGACAGGAT3820.3473737814636985No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGGCCGGTGCCCAT3540.3219118288956787No Hit
TGTAAAACGACGGCCAGTACCTGAATCTTACTAAGCGCCTGGATCTATTG3370.3064527862650953No Hit
TGTAAAACGACGGCCAGTGGGGACAAGGGTGGCTTTGTAAATAGCAGCAA3310.3009966535719482No Hit
TGTAAAACGACGGCCAGTATCTCCTGGGGAGAAACGGCATCTGTGTCCAA3130.28462825549250687No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGAGTGCAGTAGT3100.28190018914593334No Hit
TGTAAAACGACGGCCAGTTGAAGAATGGTGGCTTGTTTCATGGTTTTTGT2990.271897279208497No Hit
TGTAAAACGACGGCCAGTTGGCCCACTGTGCTAGCCTAGGATTTTTTTTT2850.25916630292448717No Hit
TGTAAAACGACGGCCAGTGTCCCAGGTGTATGGAGCGCTGGAGTGCAGTG2720.2473446820893351No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCAGCAGGATGGAG2720.2473446820893351No Hit
TGTAAAACGACGGCCAGTAGTGGAAGCTATTAGCACCAATGACATAAATA2700.2455259711916194No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGCCCTGAGAGGCGGT2610.23734177215189875No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGGGGCCCGGATGT2550.23188563945875165No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC2520.22915757311217808No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTA2420.22006401862359962No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGTGACCCTTCCAGGA2380.2164265968281682No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCCACGTGTCGCCC2170.19733013240215333No Hit
TGTAAAACGACGGCCAGTGTCCCAGGTGTATGGAGCGCCACATCTCCTGA2160.19642077695329552No Hit
TGTAAAACGACGGCCAGTGGTCCCACTCTGGCTTTCCCACCTGATAATAA2030.18459915611814345No Hit
TGTAAAACGACGGCCAGTATCTCCTGGGGAGAAACGGCTTCTGTGTCCAA1900.17277753528299142No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGAGGCTCCTTCC1860.16914011348756003No Hit
TGTAAAACGACGGCCAGTGTCCCAGGTGTATGGAGCGCAGTGGTGCGATG1770.16095591444783935No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAGCC1770.16095591444783935No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGTCAT1670.1518623599592609No Hit
TGTAAAACGACGGCCAGTAGGTGGCAAAGCTCAATCAGGACAGTTCTGAA1660.15095300451040303No Hit
TGTAAAACGACGGCCAGTGCCAAGGAGAAACTTGTGAGCATTTATTTTCA1610.14640622726611377No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT1550.14095009457296667No Hit
TGTAAAACGACGGCCAGTTCCATCCACTGCTGAGAAATCTCCTCAAACCC1550.14095009457296667No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGGGGACCTGCTGGAG1540.14004073912410883No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACTGTCCTCTCTCG1540.14004073912410883No Hit
TGTAAAACGACGGCCAGTCCTTGAAGGCTGGGCTTCTGTATGAAGGTTGG1500.13640331732867744No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGCGCCACTTGTCC1400.12730976284009893No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGCGCCTG1370.12458169649352539No Hit
TGTAAAACGACGGCCAGTAGCACAGCTGCCCTTGGATCGACATGGAGAAA1350.1227629855958097No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCGTCTACACGAGA1340.12185363014695184No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGTAAAACGAC1320.12003491924923614No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTCCCGAGCA1290.11730685290266259No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTTCCTATCG1280.11639749745380473No Hit
TGTAAAACGACGGCCAGTAGATCATCCAGCGCACACGGCTGCTGGACAGT1260.11457878655608904No Hit
TGTAAAACGACGGCCAGTCCTGGCCCACAAACTGTGCTCAGTAAATGGTA1260.11457878655608904No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGAGCCCCCTGCCG1250.1136694311072312No Hit
TGTAAAACGACGGCCAGTGCAGAAGATGGACTGTGGAGGGGGCTACATTA1230.1118507202095155No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCAGGGGGCAGTCA1200.10912265386294195No Hit
TGTAAAACGACGGCCAGTACCGGGCCAGGTCAGTTACTCCTTAAGTATGC1190.1082132984140841No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGTACCCAGTGGTGTG1180.10730394296522626No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGTGCCTG1120.10184781027207915No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)