FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003247271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003247271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398155772
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1428300.032.0664331
GATCGGA947850.030.7367781
TCGGAAG1520050.029.9943682
CGGAAGA1718100.026.6468263
TATGCCG841950.017.591945-49
CGATCAG937400.016.04784430-34
CGTATGC963300.015.58835140-44
CTCGTAT904700.015.26456740-44
TCTCGTA942300.014.54314340-44
TGCCGTC1040000.014.3938145-49
GTCACGA1049400.014.35485125-29
ATGCCGT1077600.013.99115745-49
TCGTATG1010750.013.85664540-44
GCCGTCT1094350.013.60342245-49
ACGATCA1186650.012.83471230-34
AGAGCAC3948250.012.1949197
AGTCACG1268300.012.0739625-29
AGCACAC4219350.011.4749319
ACACGTC1338200.011.33471210-14
CGTCTGA1344400.011.29751415-19
Produced by FastQC (version 0.11.5)