FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003247365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003247365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences421070527
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1498450.029.4339891
TCGGAAG1594600.027.6962782
CGGAAGA1763800.025.0635453
TATGCCG678950.013.47844745-49
CGCCAAT769500.011.88027630-34
CGTATGC808300.011.48722140-44
AGAGCAC4108700.011.2719367
CTCGTAT751050.011.0539840-44
AGCACAC4370900.010.639419
GAGCACA4493000.010.3470528
TGCCGTC901750.010.32674845-49
TCTCGTA799750.010.30834240-44
GTCACGC929150.010.03562125-29
ATGCCGT936250.010.03294845-49
GCCGTCT939750.09.958549545-49
GAAGAGC4758900.09.8689855
TATCTCG859750.09.72604735-39
ACGCCAA975100.09.59981330-34
TCGTATG922900.09.10562940-44
AGTCACG1176400.08.09151625-29
Produced by FastQC (version 0.11.5)