FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003248389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003248389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409880632
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG922700.025.3977859
GAGCGTC1221850.019.369348
ATCGGAA1511400.016.5356271
AGAGCGT1500350.015.9139897
TCGGAAG1611400.015.5166842
CGGAAGA1793750.013.9919293
AAGAGCG1828450.013.6053416
CGCCGTA422250.011.4519645-49
TCGCCGT498150.09.22451840-44
GTCGCCG566300.09.10984540-44
GCCGTAT649250.07.927829345-49
TGGTCGC710500.07.340494640-44
GGTCGCC737250.07.253024640-44
CCGTATC774500.06.821635245-49
GTGGTCG834650.06.363222640-44
CGTATCA848400.06.280384545-49
GAAGAGC4636200.05.94409475
GCGTCGT848550.05.817096710-14
CGTCGTG876300.05.713922510-14
TCGGTGG1159800.05.005223335-39
Produced by FastQC (version 0.11.5)