FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003248395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003248395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences421353177
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1435200.038.980979
GAGCGTC1745150.032.0370188
ATCGGAA2125000.030.6444531
TCGGAAG2256300.028.8123042
AGAGCGT2038950.027.6343337
CGGAAGA2433700.026.5731093
AAGAGCG2508800.025.3017396
AGCGGCG520000.021.8465189
GAGCGGC763000.015.0314128
CGCCGTA855000.013.82518245-49
ATCGGGA506500.012.9101271
GAAGAGC5344500.012.3967285
GTCGCCG1097350.011.96658640-44
AGAGCGG1002700.011.6985037
GCCGTAT1066050.011.410439545-49
TCGCCGT924000.011.04232140-44
TGGTCGC1200650.010.53011140-44
GGTCGCC1269800.010.45330440-44
CCGTATC1183000.010.37799945-49
CGTATCA1251950.09.8678145-49
Produced by FastQC (version 0.11.5)