FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003248407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003248407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417475764
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA511000.025.8877541
AGCGTCG949700.025.2770589
AGCGGCG420400.024.0410759
GAGCGTC1247650.019.3863228
ATCGGAA1582900.019.164051
TCGGAAG1675050.018.0796622
CGGAAGA1858600.016.3844833
AGAGCGT1530500.016.0499177
GAGCGGC642650.015.8512668
AAGAGCG1883600.015.2233446
CGCCGTA422600.012.40703845-49
AGAGCGG854250.012.26437957
CGGGAGA1201050.011.0956783
GTCGCCG570000.010.24166140-44
TCCGATC304850.08.7505141
TCGCCGT486250.08.73734440-44
GCCGTAT660050.08.52142345-49
GGTCGCC729700.08.18301740-44
TGGTCGC719950.07.88699940-44
GAGAGCG876000.07.6723416
Produced by FastQC (version 0.11.5)