FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003248414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003248414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446647861
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG11231540.2514629752139348TruSeq Adapter, Index 16 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2859500.074.120761
TCGGAAG2972750.071.198362
CGGAAGA3167750.066.742873
AGAGCAC5668450.037.500887
AGCACAC5913700.035.9273539
GAGCACA6084850.034.9406438
GAAGAGC6317900.033.7997475
AAGAGCA8864500.024.335086
TATGCCG1931500.022.38585545-49
GGAAGAG9940400.021.8283024
CCCGATC2029850.021.10448335-39
CGTATGC2046500.020.89973445-49
CGTCCCG1988200.020.85637330-34
CTCGTAT1868950.020.41341440-44
TCCCGAT2095250.020.37238735-39
GTCCCGA2096350.020.24342735-39
CCGATCT1876550.020.05708135-39
TCTCGTA1904850.019.9137540-44
ATGCCGT2182300.019.66431245-49
GCCGTCT2202850.019.14700950-54
Produced by FastQC (version 0.11.5)