FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003248415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003248415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446647861
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1753500.065.6445169
AGCGGCG800100.062.159439
ATCGGGA838800.058.8184361
ATCGGAA2607800.056.734611
GAGCGTC2088200.054.9039768
TCGGAAG2729900.053.5224342
CGGAAGA2932650.049.7393
AGAGCGT2368150.048.589637
AAGAGCG2934250.047.3328066
GAGCGGC1060900.045.682638
AGAGCGG1311600.037.8760347
CGGGAGA1638900.028.7105123
GAAGAGC6010050.023.5929725
GAGAGCG1204300.021.8187926
CGCCGTA1085000.020.702145-49
GTCGCCG1324100.018.5791140-44
GCCGTAT1335050.017.34918845-49
TCGCCGT1089150.017.34090240-44
AGGGGCG1565750.016.7963779
GGTCGCC1503800.016.58069840-44
Produced by FastQC (version 0.11.5)