FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences316810284
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1920700.023.7763161
TCGGAAG2018350.022.7292962
CGGAAGA2146900.021.4189683
AGAGCAC3594500.013.2364997
AGCACAC3878050.012.4388179
GAGCACA4085200.011.713998
GAAGAGC4250100.011.3515745
CTCGTAT975750.09.43863240-44
TCTCGTA1016350.09.06444240-44
TATGCCG1053950.09.01057845-49
AAGAGCA5782950.08.5995996
TATCTCG1073500.08.5062340-44
ATGCCGT1148950.08.3816645-49
GGAAGAG6452350.07.8085164
ATCTCGT1218800.07.639689440-44
CGTATGC1300550.07.363388545-49
TGCCGTC1341700.07.24130945-49
GCCGTCT1357700.07.050983450-54
TCGTATG1493250.06.48708340-44
CGTCTGA1563650.06.14334315-19
Produced by FastQC (version 0.11.5)