FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409497469
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT16566060.40454609012491843Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGGCG1373350.093.802389
ATCGGGA1379250.092.9119641
GAGCGGC1608450.078.619298
AGCGTCG5887250.071.8406459
ATCGGAA7603500.067.787931
GAGCGTC6263250.067.330588
AGAGCGG1914550.067.162967
TCGGAAG7698900.066.383982
CGGAAGA7800400.065.3311163
AAGAGCG7601100.064.203546
AGAGCGT6640650.063.7824827
CGGGAGA2254850.054.7994843
GAAGAGC10462450.046.9379655
GAGAGCG1536150.042.7477266
AGGGGCG1782950.039.7588739
GGAAGAG16363750.031.6154754
TCGGGAG4542650.028.5771142
AGGGTCG662100.023.022379
CGCCGGA1109200.022.58587845-49
CCGGATC1352900.022.03743645-49
Produced by FastQC (version 0.11.5)