FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410670835
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1065200.026.2497259
GAGCGTC1377250.020.3917148
ATCGGAA1663450.018.0416681
TCGGAAG1784150.016.7294672
AGAGCGT1729700.016.4297287
CGGAAGA1991500.014.9145793
AAGAGCG2131300.013.8509096
CGCCGTA573750.010.21543245-49
TCGCCGT662100.08.51041940-44
GTCGCCG766050.08.18086740-44
GCCGTAT812950.07.63597545-49
CCGTATC918250.06.888232745-49
TGGTCGC937900.06.83650140-44
GGTCGCC942650.06.80512940-44
GAAGAGC4821850.06.6920155
CGTATCA996200.06.43658245-49
GTGGTCG1045600.06.15312340-44
GCGTCGT967200.06.096695410-14
CGTCGTG1003950.05.961619410-14
TCCGATC364150.05.39633851
Produced by FastQC (version 0.11.5)