FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357574664
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTATGCCGTCTTCTGCTT237864536.652163979940144RNA PCR Primer, Index 41 (96% over 27bp)
ACGTCTGAACTCCAGTCACTCCGCGAAATCCCGTATGCCGTCTTCTGCTT9644630.26972352828666857RNA PCR Primer, Index 19 (96% over 25bp)
ACGTCTGAACTCCAGTCACTCCGCGAAATCGCGTATGCCGTCTTCTGCTT3926480.10980867481147936RNA PCR Primer, Index 19 (96% over 25bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA33233350.0121.9061362
ACGTCTG33259800.0121.544391
TCTGAAC35176750.0114.976114
GTCTGAA35686650.0113.597513
CTGAACT36067950.0112.240715
AACTCCA36502650.0110.548978
GAACTCC37713800.0107.2263647
TGAACTC37976950.0106.532896
ACTCCAG41075050.098.045729
GCCGTCT30287700.026.69507835-39
TGCCGTC30325350.026.64811935-39
TATGCCG30315000.026.62922530-34
CGTATGC30495000.026.62396430-34
TCGTATG28413600.026.58458330-34
CCGTCTT30414650.026.5722635-39
CGTCTTC30288100.026.46718435-39
TCTCGTA28453050.026.44813725-29
ATCTCGT28685300.026.41153125-29
CTCGTAT28389750.026.38794930-34
ATGCCGT30362050.026.37483235-39
Produced by FastQC (version 0.11.5)