FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences439397948
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA860350.065.519331
AGCGGCG757400.063.9109429
AGCGTCG2024750.050.85799
GAGCGGC1007800.047.592718
GAGCGTC2366750.043.5670478
ATCGGAA2951750.043.499541
TCGGAAG3044050.041.9370272
AGAGCGG1238850.039.6411177
CGGAAGA3210750.039.5705263
AGAGCGT2669150.038.951657
AAGAGCG3215150.038.1198966
CGGGAGA1614400.033.6856163
GAGAGCG1152800.027.0286586
AGGGGCG1378750.021.1325389
GAAGAGC6178550.020.3105965
CGCCGTA1090200.018.43438745-49
TCGGGAG3300200.017.3368822
GTCGCCG1332400.016.9240140-44
GCCGTAT1331450.015.61581345-49
TCGCCGT1114150.015.47744940-44
Produced by FastQC (version 0.11.5)