FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310215355
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1770300.014.5894111
TCGGAAG1873050.013.8835552
CGGAAGA2001750.013.0525493
AGAGCAC3397450.08.0626327
AGCACAC3640150.07.64660559
GAGCACA3893800.07.15589868
GAAGAGC4031150.07.0001145
GCGCATC789400.06.759341235-39
CTCGTAT790450.06.741617740-44
TAATGCG837800.06.425413630-34
TCTCGTA820750.06.36906340-44
TATGCCG871750.06.36801245-49
AATGCGC861600.06.207539630-34
TGCGCAT896300.06.001698535-39
ATGCCGT964650.05.84043545-49
ATGCGCA935700.05.831109535-39
CGCATCT944900.05.62088535-39
AAGAGCA5535650.05.31752356
ATCTCGT1018350.05.30408440-44
CGTATGC1112600.05.084646745-49
Produced by FastQC (version 0.11.5)