FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287044393
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1880100.017.156551
TCGGAAG1957300.016.5617082
CGGAAGA2083950.015.5203883
AGAGCAC3405700.09.9025877
AGCACAC3535700.09.5794819
GAGCACA3866200.08.8767938
GAAGAGC3934950.08.7345515
TATGCCG965450.08.48796753
ATGCCGT1035250.08.0557624
CTCGTAT848950.07.715327740-44
CGGCTAT894900.07.481644630-34
TCTCGTA889600.07.40841940-44
TGCCGTC1241900.06.9086275
GCCGTCT1260300.06.8652616
AAGAGCA5331800.06.69766666
ACCGGCT1051800.06.40831830-34
ATCTCGT1073550.06.290286540-44
TCACCGG1096050.06.155839425-29
GGAAGAG6040750.06.0433314
GTCACCG1158050.05.90012825-29
Produced by FastQC (version 0.11.5)