FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287044393
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1580350.020.3220569
GAGCGTC1981100.016.39068
ATCGGAA2172200.014.8480451
AGAGCGT2239250.014.578767
TCGGAAG2261850.014.356372
CGGAAGA2354800.013.8321633
AAGAGCG2566550.012.8521366
CGCCGTA746700.08.86553555-59
TCGCCGT828700.08.0932655-59
GAAGAGC4359250.07.9542285
GCCGTAT889550.07.56408555-59
CCGTATC970350.06.950667455-59
CGTATCA996800.06.89696260-64
GTCGCCG1087000.06.391495755-59
GTGGTCG1238500.05.685804450-54
TGGTCGC1232050.05.675560550-54
GGAAGAG6686350.05.518674
TCTCGGT1379000.05.18861145-49
GGTCGCC1379400.05.165984650-54
TCGGTGG1402450.05.051196645-49
Produced by FastQC (version 0.11.5)