FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445264148
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG4656050.10456826629571803TruSeq Adapter, Index 18 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1766150.051.637921
TCGGAAG1898600.047.9264222
CGGAAGA2102900.043.3520433
AGAGCAC4605850.020.3598067
ACGTCCG975600.019.22769530-34
AGCACAC4886800.019.18849
GAGCACA5028250.018.6698448
TATGCCG1047950.018.11769345-49
GAAGAGC5245550.017.9418985
CGTCCGC1059750.017.76931230-34
GTCCGCA1153550.016.32818630-34
CGTATGC1159050.016.31473445-49
CTCGTAT1037750.015.40738640-44
TCCGCAC1213900.014.80819430-34
TCTCGTA1072350.014.7791140-44
ATGCCGT1316100.014.37671445-49
GCCGTCT1346300.013.954988550-54
TGCCGTC1297200.013.87866745-49
CGCACAT1367000.013.68332835-39
CCGCACA1433850.013.22636135-39
Produced by FastQC (version 0.11.5)