FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490948331
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG12919320.2631502988040507TruSeq Adapter, Index 7 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA5352900.038.6895521
TCGGAAG5490100.037.889652
CGGAAGA5588700.037.2111553
AGAGCAC7811400.026.9065847
AGCACAC8106200.025.9378729
GAGCACA8457200.024.9025218
GAAGAGC8762400.024.0675955
AAGAGCA11202000.019.0219696
GGAAGAG12237100.017.5190544
CTCGTAT2862550.014.09024840-44
TATGCCG3036800.013.85554445-49
TCTCGTA2925600.013.78658840-44
TCGTATC3059950.013.73750935-39
ATGCCGT3167750.013.26309845-49
TATCTCG3037550.013.14388940-44
CGTATCT3247000.012.50502435-39
ATCTCGT3238450.012.48025240-44
CGTATGC3401400.012.2889445-49
TGCCGTC3416400.012.27360345-49
ATTCGTA3417050.012.25180535-39
Produced by FastQC (version 0.11.5)