FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490948331
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC13362980.27218709497965476Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG5013200.041.2077839
GAGCGTC5615300.036.8939638
AGAGCGT6113450.033.949347
ATCGGAA6200200.033.3758241
TCGGAAG6374600.032.6662642
CGGAAGA6470500.032.1455543
AAGAGCG6823800.030.5852266
GAAGAGC9873350.021.43745
GGAAGAG13994900.015.3881174
CGCCGTA3139500.013.31196655-59
TCGCCGT3228700.012.87189755-59
GCCGTAT3340350.012.53584455-59
CCGTATC3464100.012.07755555-59
CGTATCA3492350.012.00885960-64
GTCGCCG3682800.011.45403555-59
TGGTCGC3902550.010.89469350-54
GTGGTCG3983850.010.72658450-54
GGTCGCC4097000.010.42078750-54
TCTCGGT4330250.09.97983645-49
TCGGTGG4391250.09.80851845-49
Produced by FastQC (version 0.11.5)