FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences484864033
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATG27889600.5752045543044023TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA6158250.074.1411
TCGGAAG6383150.071.800512
CGGAAGA6539500.070.1582643
AGAGCAC8757400.052.4633187
AGCACAC9162650.050.2372069
GAGCACA9451300.048.6868178
GAAGAGC9658350.047.7624475
AAGAGCA11945900.038.7186556
GGAAGAG12960450.035.86244
CTCGTAT4289200.020.36287940-44
TCTCGTA4355000.020.04090140-44
TATGCCG4606700.020.03567745-49
ATGCCGT4738350.019.44902445-49
GTCACGA4846950.018.99290825-29
ATCTCGT4694200.018.63290440-44
ACGAGAT4948200.018.60525930-34
CGTATGC4984250.018.37111345-49
TGCCGTC5046000.018.2218645-49
GCCGTCT5072950.017.96642350-54
TCGTATG5021500.017.42736640-44
Produced by FastQC (version 0.11.5)