FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences484864033
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC29179610.6018101573642606Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG6147950.074.275449
GAGCGTC6726100.067.976698
ATCGGAA7064050.064.725161
AGAGCGT7208150.063.3026167
TCGGAAG7341200.062.493672
CGGAAGA7451050.061.4965483
AAGAGCG7910100.057.8513876
GAAGAGC10851650.042.5253375
GGAAGAG14950500.031.1043074
CGCCGTA4637050.019.76075755-59
TCGCCGT4754000.019.28500255-59
GCCGTAT4835600.018.98295855-59
CCGTATC4956750.018.43679855-59
CGTATCA4987550.018.29167260-64
GTCGCCG5193200.017.70176755-59
TGGTCGC5398100.017.10393350-54
GTGGTCG5483650.016.85956850-54
GGTCGCC5603100.016.47789650-54
TCTCGGT5800700.016.01021245-49
TCGGTGG5907300.015.60127645-49
Produced by FastQC (version 0.11.5)