FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339202244
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG5747690.16944728702915066TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG4516500.020.5456392
ATCGGAA4493650.020.4900551
CGGAAGA4602250.020.1140383
AGAGCAC5694600.016.4786037
AGCACAC5948100.015.8335979
GAGCACA6112900.015.4327378
GAAGAGC6488650.014.5803425
AAGAGCA8018450.011.9594046
GGAAGAG8809050.010.9962314
CTCGTAT1796150.010.15423240-44
TATGCCG1867150.010.10555945-49
TCTCGTA1849750.09.91013640-44
ATGCCGT1973750.09.58767245-49
ATCTCGT2077450.08.85950840-44
TGCCGTC2140000.08.8198145-49
CGTATGC2152500.08.70799345-49
GCCGTCT2148100.08.69701750-54
CCGGAGA2300050.08.17157130-34
CGTCTTC2411300.08.03199450-54
TCGTATG2372750.07.92425440-44
Produced by FastQC (version 0.11.5)