FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369125165
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCTC32503760.880562017493443Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG16177700.030.5597749
GAGCGTC16732800.029.5661328
AGAGCGT17284600.028.6687167
AAGAGCG18034450.027.5077866
CGGAAGA18072550.027.4610273
TCGGAAG18155500.027.3401412
ATCGGAA18186550.027.2578811
GAAGAGC20123800.024.7616465
GGAAGAG23057750.021.7407044
CGCCGTA9274600.010.93740355-59
CGTATCA9340700.010.87673260-64
GCCGTAT9361200.010.81391855-59
TCGCCGT9446950.010.76195855-59
CCGTATC9390050.010.65531155-59
GTCGCCG9819350.010.35336655-59
TGGTCGC10182950.09.96997550-54
GGTCGCC10172150.09.86597250-54
GTGGTCG10394500.09.82578450-54
TCTCGGT11061700.09.23934745-49
GTATCAT11284800.09.2236560-64
Produced by FastQC (version 0.11.5)