FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366746750
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG38472751.0490277009953053TruSeq Adapter, Index 7 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA16624100.035.867843
TCGGAAG16715800.035.7053072
ATCGGAA16741000.035.6120151
AGAGCAC17455450.034.1571127
AGCACAC17620650.033.827469
GAGCACA17776550.033.5375068
GAAGAGC18409050.032.470855
AAGAGCA20099450.029.7930036
GGAAGAG21027850.028.5241014
TATGCCG10020650.012.11375845-49
ATGCCGT10004850.012.08259245-49
CGTCTTC10167950.011.94495650-54
CTCGTAT9968550.011.94215840-44
GCCGTCT10057650.011.90768750-54
TCTCGTA10107250.011.81127240-44
TGCCGTC10122000.011.78787745-49
CGTATGC10458450.011.49837245-49
CCGTCTT10638400.011.38919250-54
ATCTCGT10479800.011.359982540-44
TATCTCG10421950.011.32815940-44
Produced by FastQC (version 0.11.5)