FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249703

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249703
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188864986
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG353650.049.7083639
GAGCGTC538300.032.9802258
ATCGGAA571800.032.949991
TCGGAAG607000.031.0184882
CGGAAGA681000.027.762313
AGAGCGT656000.027.360577
AAGAGCG792000.023.907076
CGCCGTA214900.016.09024455-59
TCGCCGT242850.013.92198155-59
GCCGTAT325800.010.82238955-59
GTCGCCG354850.010.230576555-59
CCGTATC359850.09.80237355-59
GAAGAGC2078550.09.7650725
CGTATCA415250.08.71836860-64
CGTCGTG421850.08.66061210-14
TGGTCGC428700.08.52594450-54
GTGGTCG441250.08.38859450-54
TCTCGGT516150.07.6712345-49
GCGTCGT488850.07.62190710-14
GGTCGCC488200.07.620473450-54
Produced by FastQC (version 0.11.5)