FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390803091
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT8840280.22620803682435564Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2974200.048.267739
GAGCGTC3321700.043.3872388
AGAGCGT3675500.039.354077
ATCGGAA3857000.038.9231831
TCGGAAG3947200.037.869022
CGGAAGA4124100.036.130483
AAGAGCG4125250.035.7533956
GAAGAGC6650550.022.6228985
CGCCGTA1672650.017.99320845-49
GTCGCCG1895750.016.35366840-44
GCCGTAT1883450.016.13101645-49
TCGCCGT1782950.015.91725140-44
GGAAGAG9982250.015.4717984
CCGTATC1977700.015.44511245-49
CGTATCA2044450.015.040832545-49
GGTCGCC2090050.014.92354340-44
TGGTCGC2108450.014.66953340-44
GTGGTCG2256050.013.80361940-44
TCGGTGG2672250.011.97090435-39
TCTCGGT2656450.011.95041435-39
Produced by FastQC (version 0.11.5)