FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409049076
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1385000.030.3734171
TCGGAAG1490250.028.1743552
CGGAAGA1682850.024.96253
TATGCCG674150.013.2522545-49
CGTTTCG710800.012.42369630-34
CGTATGC785900.011.2405545-49
AGAGCAC3940550.011.1337277
CTCGTAT746150.010.75670640-44
AGCACAC4159450.010.6715149
GAGCACA4316250.010.3039918
TCTCGTA786350.010.26579940-44
TCGGAAT888550.010.09334235-39
GAAGAGC4544750.09.8225885
ATGCCGT938250.09.72754245-49
GTTTCGG932900.09.72545830-34
TGCCGTC887900.09.60466245-49
GCCGTCT929300.09.58096650-54
TTCGGAA994750.09.04637835-39
TCGTATG908050.08.49717340-44
ATCTCGT1001900.08.28583640-44
Produced by FastQC (version 0.11.5)