FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398123606
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2194550.030.0957831
TCGGAAG2310100.028.7080752
CGGAAGA2466600.026.882283
AGAGCAC4630900.014.8081937
AGCACAC4861550.014.11607559
TATGCCG976000.013.78426345-49
GAGCACA5004350.013.7393418
GAAGAGC5225650.013.2186325
CGTATGC1097750.012.45222640-44
CTCGTAT1026450.011.77488840-44
TGCCGTC1197750.011.45138545-49
ATGCCGT1224350.011.23101845-49
GCCGTCT1235000.011.12829645-49
TCTCGTA1075750.011.11801840-44
TCGTATG1160950.010.47815940-44
AAGAGCA7441750.09.586996
CGTCTTC1475950.09.3048650-54
ATCTCGT1301550.09.08802135-39
TCACGAT1487250.09.04433530-34
CCGTCTT1525100.08.85462645-49
Produced by FastQC (version 0.11.5)