FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398123606
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT4024130.10107740257933864Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1835400.035.5288629
GAGCGTC2173700.030.0583388
ATCGGAA2561900.026.2315351
AGAGCGT2522800.026.1190687
TCGGAAG2683800.025.0617452
CGGAAGA2877350.023.2626363
AAGAGCG2944650.022.6850096
CGCCGTA961900.013.72163945-49
GAAGAGC5531850.012.572485
TCGCCGT1066700.012.12191340-44
GTCGCCG1158350.011.74980440-44
GCCGTAT1187100.011.371366545-49
CCGTATC1290150.010.55972745-49
GGTCGCC1345300.010.22798640-44
CGTATCA1352700.010.14217445-49
TGGTCGC1352200.010.12647640-44
GTGGTCG1475700.09.32812140-44
GGAAGAG8866600.08.2181194
GCGTCGT1739450.07.681563410-14
TCTCGGT1870100.07.656301535-39
Produced by FastQC (version 0.11.5)