FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003249721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9287597
Sequences flagged as poor quality0
Sequence length101-126
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA338890.36488447980677885No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT300840.32391586327442934No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT191360.20603822495743515No Hit
TGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCT147670.15899699351726826No Hit
GGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCAC144330.15540079958249697No Hit
GCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCG138120.1487144629552725No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC138090.1486821618121458No Hit
GATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTC131800.1419096888032502No Hit
GTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCT115760.1246393442781809No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG115200.12403638960648271No Hit
CTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTC111930.1205155650056737No Hit
GTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAAT105340.11342008056551117No Hit
TGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCT101310.10908096033882607No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT98970.1065614711749444No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA96370.1037620387706314No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)